Phosphorylated B6 vitamer deficiency in SALT OVERLY SENSITIVE 4 mutants compromises shoot and root development
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| Názov: | Phosphorylated B6 vitamer deficiency in SALT OVERLY SENSITIVE 4 mutants compromises shoot and root development |
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| Autori: | Gorelova, Vera, Colinas Martinez, Maite Felicitas, Dell'Aglio, Elisa, Flis, Paulina, Salt, David E, Fitzpatrick, Thérésa Bridget |
| Zdroj: | Plant Physiology. 188:220-240 |
| Informácie o vydavateľovi: | Oxford University Press (OUP), 2021. |
| Rok vydania: | 2021 |
| Predmety: | 0301 basic medicine, Genotype, Plant Roots / growth & development, Salt Stress / genetics, Arabidopsis, Genes, Plant, Plant Roots, Salt Stress, Plant Shoots / genetics, Organogenesis, Plant, 03 medical and health sciences, Gene Expression Regulation, Plant, Plant Roots / genetics, 0303 health sciences, Genetic Variation, Salt Tolerance, Pyridoxal Phosphate / metabolism, Plant Shoots / growth & development, Organogenesis, Plant / genetics, Pyridoxal Phosphate / genetics, Arabidopsis / genetics, Pyridoxal Phosphate, Mutation, Arabidopsis / growth & development, Plant Shoots, Salt Tolerance / genetics |
| Popis: | Stunted growth in saline conditions is a signature phenotype of the Arabidopsis SALT OVERLY SENSITIVE mutants (sos1-5) affected in pathways regulating the salt stress response. One of the mutants isolated, sos4, encodes a kinase that phosphorylates pyridoxal (PL), a B6 vitamer, forming the important coenzyme pyridoxal 5′-phosphate (PLP). Here, we show that sos4-1 and more recently isolated alleles are deficient in phosphorylated B6 vitamers including PLP. This deficit is concomitant with a lowered PL level. Ionomic profiling of plants under standard laboratory conditions (without salt stress) reveals that sos4 mutants are perturbed in mineral nutrient homeostasis, with a hyperaccumulation of transition metal micronutrients particularly in the root, accounting for stress sensitivity. This is coincident with the accumulation of reactive oxygen species, as well as enhanced lignification and suberization of the endodermis, although the Casparian strip is intact and functional. Further, micrografting shows that SOS4 activity in the shoot is necessary for proper root development. Growth under very low light alleviates the impairments, including salt sensitivity, suggesting that SOS4 is important for developmental processes under moderate light intensities. Our study provides a basis for the integration of SOS4 derived B6 vitamers into plant health and fitness. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1093/plphys/kiab475 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/34730814 https://europepmc.org/article/MED/34730814 |
| Rights: | OUP Standard Publication Reuse |
| Prístupové číslo: | edsair.doi.dedup.....5861db4203f45e2598b5bd621e3639cd |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Stunted growth in saline conditions is a signature phenotype of the Arabidopsis SALT OVERLY SENSITIVE mutants (sos1-5) affected in pathways regulating the salt stress response. One of the mutants isolated, sos4, encodes a kinase that phosphorylates pyridoxal (PL), a B6 vitamer, forming the important coenzyme pyridoxal 5′-phosphate (PLP). Here, we show that sos4-1 and more recently isolated alleles are deficient in phosphorylated B6 vitamers including PLP. This deficit is concomitant with a lowered PL level. Ionomic profiling of plants under standard laboratory conditions (without salt stress) reveals that sos4 mutants are perturbed in mineral nutrient homeostasis, with a hyperaccumulation of transition metal micronutrients particularly in the root, accounting for stress sensitivity. This is coincident with the accumulation of reactive oxygen species, as well as enhanced lignification and suberization of the endodermis, although the Casparian strip is intact and functional. Further, micrografting shows that SOS4 activity in the shoot is necessary for proper root development. Growth under very low light alleviates the impairments, including salt sensitivity, suggesting that SOS4 is important for developmental processes under moderate light intensities. Our study provides a basis for the integration of SOS4 derived B6 vitamers into plant health and fitness. |
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| ISSN: | 15322548 00320889 |
| DOI: | 10.1093/plphys/kiab475 |