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The Effect of Cultured Autologous Periodontal Ligament Cells on the Healing of Delayed Autotransplanted Dog's Teeth

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Bibliographic Details
Title: The Effect of Cultured Autologous Periodontal Ligament Cells on the Healing of Delayed Autotransplanted Dog's Teeth
Authors: Yixiang Wang, Xiangliang Xu, Chengfei Zhang, Gary S.P. Cheung, Shijie Zhao
Source: Journal of Endodontics. 36:264-267
Publisher Information: Elsevier BV, 2010.
Publication Year: 2010
Subject Terms: Periodontium, 0301 basic medicine, replantation, Time Factors, Tissue Engineering - methods, Cell Transplantation, Periodontal Ligament, Cell Culture Techniques, Tooth Replantation - methods, Transplantation, Autologous, Hydrogel, Polyethylene Glycol Dimethacrylate, 03 medical and health sciences, Dogs, 0302 clinical medicine, Periodontal Ligament - cytology - transplantation, periodontal regeneration, Animals, Periodontal - methods, Bicuspid, Delayed autotransplantation, Guided Tissue Regeneration, Periodontal - methods, Tooth, Nonvital, Tissue Engineering, Tissue Scaffolds, Guided Tissue Regeneration, periodontal ligament, Graft Survival, Guided Tissue Regeneration, Periodontal, Tooth Replantation
Description: The regeneration of the periodontal structure for avulsed teeth extended dry times has been a goal of dentists. The aim of this study was to investigate a new strategy of delayed replantation for avulsed teeth that were not suitable for immediate replantation.Extracted dog's premolar teeth were maintained in a dry environment for a month after isolation and proliferation of the periodontal ligament (PDL) cells. Then, tooth roots coated with 1 x 10(6) cultured autologous PDL cells were autotransplanted in artificial sockets created in the mandible. The dogs were sacrificed 60 days after transplantation. Histologic analyses showed that a root-PDL-bone complex was found in all cases of the PDL cell-loaded samples.The new PDL-like connective tissue was located between the alveolar bone and the transplanted roots, with fibers inserting into the newborn cementum on one end and alveolar bone on the other. For the control samples, no PDL-like tissue was found, and ankylosis was commonly observed.The results indicated that cultured autologous PDL cells assist in the re-establishment of periodontal architecture of autotransplanted teeth that is devoid of viable periodontal cells.
Document Type: Article
Language: English
ISSN: 0099-2399
DOI: 10.1016/j.joen.2009.09.014
Access URL: http://hub.hku.hk/bitstream/10722/124104/2/Content.pdf
https://pubmed.ncbi.nlm.nih.gov/20113786
https://www.ncbi.nlm.nih.gov/pubmed/20113786
https://www.sciencedirect.com/science/article/pii/S0099239909007742
https://pubmed.ncbi.nlm.nih.gov/20113786/
https://core.ac.uk/display/37949780
http://hub.hku.hk/bitstream/10722/124104/2/Content.pdf
https://europepmc.org/abstract/MED/20113786
http://hdl.handle.net/10722/124104
Rights: Elsevier TDM
CC BY NC ND
Accession Number: edsair.doi.dedup.....570b22846e554d38b84e28e665fbb7d4
Database: OpenAIRE
Description
Abstract:The regeneration of the periodontal structure for avulsed teeth extended dry times has been a goal of dentists. The aim of this study was to investigate a new strategy of delayed replantation for avulsed teeth that were not suitable for immediate replantation.Extracted dog's premolar teeth were maintained in a dry environment for a month after isolation and proliferation of the periodontal ligament (PDL) cells. Then, tooth roots coated with 1 x 10(6) cultured autologous PDL cells were autotransplanted in artificial sockets created in the mandible. The dogs were sacrificed 60 days after transplantation. Histologic analyses showed that a root-PDL-bone complex was found in all cases of the PDL cell-loaded samples.The new PDL-like connective tissue was located between the alveolar bone and the transplanted roots, with fibers inserting into the newborn cementum on one end and alveolar bone on the other. For the control samples, no PDL-like tissue was found, and ankylosis was commonly observed.The results indicated that cultured autologous PDL cells assist in the re-establishment of periodontal architecture of autotransplanted teeth that is devoid of viable periodontal cells.
ISSN:00992399
DOI:10.1016/j.joen.2009.09.014