Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica
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| Název: | Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica |
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| Autoři: | Jung-Kul Lee, Tae-Su Kim, Kyoung-Mi Lee, In-Won Kim, Saurabh Sudha Dhiman, Dayanand Kalyani, Manish Kumar Tiwari |
| Přispěvatelé: | College of Medicine, Dept. of Life Science, Kyoung-Mi Lee, Dayanand Kalyani, Manish Kumar Tiwari, Tae-Su Kim, Saurabh Sudha Dhiman, Jung-Kul Lee, In-Won Kim, Lee, Kyoung Mi |
| Zdroj: | Bioresource Technology. 123:636-645 |
| Informace o vydavateli: | Elsevier BV, 2012. |
| Rok vydání: | 2012 |
| Témata: | Laccase/metabolism, Yarrowia/enzymology, 0106 biological sciences, 0301 basic medicine, Evolution, Molecular Sequence Data, Oryza/chemistry, Cellulase/metabolism, Peptides/chemistry, Metals/pharmacology, 01 natural sciences, Substrate Specificity, Phenols/isolation & purification, Evolution, Molecular, 03 medical and health sciences, Yarrowia/isolation & purification, Cellulase, Phenols, Waste Products/analysis, Amino Acid Sequence, Enzyme Inhibitors, Hydrolysis/drug effects, Phylogeny, Enzyme Inhibitors/pharmacology, Ions, Waste Products, Hydrolysis, Laccase, Molecular, Reproducibility of Results, Oryza, Carbohydrate Metabolism/drug effects, Enzyme Activation, Kinetics, Environmental/drug effects, Biodegradation, Environmental, Metals, Biodegradation, Carbohydrate Metabolism, Enzyme Activation/drug effects, Peptides, Laccase/isolation & purification, Substrate Specificity/drug effects |
| Popis: | An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k(cat)/K(m)=19.3s(-1)μM(-1)) and 2,6-dimethoxyphenol (k(cat)/K(m)=13s(-1)μM(-1)) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2012.07.066 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/22960123 https://www.sciencedirect.com/science/article/pii/S0960852412011133 https://ir.ymlib.yonsei.ac.kr/handle/22282913/158378 https://www.ncbi.nlm.nih.gov/pubmed/22960123 https://orbit.dtu.dk/en/publications/enhanced-enzymatic-hydrolysis-of-rice-straw-by-removal-of-phenolic-compounds-using-a-novel-laccase-from-yeast-yarrowia-lipolytica(43fc26b3-8183-49cd-8426-de6df47133dd).html https://pubmed.ncbi.nlm.nih.gov/22960123/ https://orbit.dtu.dk/en/publications/enhanced-enzymatic-hydrolysis-of-rice-straw-by-removal-of-phenoli |
| Rights: | Elsevier TDM CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....56fc02f33f74a4070fb4e3f3ec535c88 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k(cat)/K(m)=19.3s(-1)μM(-1)) and 2,6-dimethoxyphenol (k(cat)/K(m)=13s(-1)μM(-1)) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries. |
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| ISSN: | 09608524 |
| DOI: | 10.1016/j.biortech.2012.07.066 |