High-resolution association mapping with libraries of immortalized lines from ancestral landraces
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| Titel: | High-resolution association mapping with libraries of immortalized lines from ancestral landraces |
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| Autoren: | Tobias Würschum, Thea M. Weiß, Juliane Renner, H. Friedrich Utz, Alfons Gierl, Rafal Jonczyk, Lilla Römisch-Margl, Wolfgang Schipprack, Chris-Carolin Schön, Tobias A. Schrag, Willmar L. Leiser, Albrecht E. Melchinger |
| Quelle: | Theor Appl Genet |
| Verlagsinformationen: | Springer Science and Business Media LLC, 2021. |
| Publikationsjahr: | 2021 |
| Schlagwörter: | 0301 basic medicine, 2. Zero hunger, 0303 health sciences, Original Article, Plant Breeding/Biotechnology, Plant Genetics and Genomics, Agriculture, Plant Biochemistry, Biochemistry, general, Biotechnology, Quantitative Trait Loci, 15. Life on land, Genes, Plant, Zea mays, Linkage Disequilibrium, ddc, Plant Breeding, 03 medical and health sciences, Phenotype, Species Specificity, Genetic Association Studies, Gene Library |
| Beschreibung: | Key messageAssociation mapping with immortalized lines of landraces offers several advantages including a high mapping resolution, as demonstrated here in maize by identifying the causal variants underlying QTL for oil content and the metabolite allantoin.AbstractLandraces are traditional varieties of crops that present a valuable yet largely untapped reservoir of genetic variation to meet future challenges of agriculture. Here, we performed association mapping in a panel comprising 358 immortalized maize lines from six European Flint landraces. Linkage disequilibrium decayed much faster in the landraces than in the elite lines included for comparison, permitting a high mapping resolution. We demonstrate this by fine-mapping a quantitative trait locus (QTL) for oil content down to the phenylalanine insertion F469 inDGAT1-2as the causal variant. For the metabolite allantoin, related to abiotic stress response, we identified promoter polymorphisms and differential expression of an allantoinase as putative cause of variation. Our results demonstrate the power of this approach to dissect QTL potentially down to the causal variants, toward the utilization of natural or engineered alleles in breeding. Moreover, we provide guidelines for studies using ancestral landraces for crop genetic research and breeding. |
| Publikationsart: | Article Other literature type |
| Dateibeschreibung: | application/pdf |
| Sprache: | English |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s00122-021-03963-3 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/34668978 https://link.springer.com/article/10.1007/s00122-021-03963-3 https://link.springer.com/content/pdf/10.1007/s00122-021-03963-3.pdf https://mediatum.ub.tum.de/doc/1705253/document.pdf |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . |
| Dokumentencode: | edsair.doi.dedup.....53fbbfe032fc8f9fb1e6b4197632ea70 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Key messageAssociation mapping with immortalized lines of landraces offers several advantages including a high mapping resolution, as demonstrated here in maize by identifying the causal variants underlying QTL for oil content and the metabolite allantoin.AbstractLandraces are traditional varieties of crops that present a valuable yet largely untapped reservoir of genetic variation to meet future challenges of agriculture. Here, we performed association mapping in a panel comprising 358 immortalized maize lines from six European Flint landraces. Linkage disequilibrium decayed much faster in the landraces than in the elite lines included for comparison, permitting a high mapping resolution. We demonstrate this by fine-mapping a quantitative trait locus (QTL) for oil content down to the phenylalanine insertion F469 inDGAT1-2as the causal variant. For the metabolite allantoin, related to abiotic stress response, we identified promoter polymorphisms and differential expression of an allantoinase as putative cause of variation. Our results demonstrate the power of this approach to dissect QTL potentially down to the causal variants, toward the utilization of natural or engineered alleles in breeding. Moreover, we provide guidelines for studies using ancestral landraces for crop genetic research and breeding. |
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| ISSN: | 14322242 00405752 |
| DOI: | 10.1007/s00122-021-03963-3 |