HIRA, a Conserved Histone Chaperone, Plays an Essential Role in Low-dose Stress Response via Transcriptional Stimulation in Fission Yeast
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| Názov: | HIRA, a Conserved Histone Chaperone, Plays an Essential Role in Low-dose Stress Response via Transcriptional Stimulation in Fission Yeast |
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| Autori: | Chujo, Moeko, Tarumoto, Yusuke, Miyatake, Koichi, Nishida, Eisuke, Ishikawa, Fuyuki |
| Prispievatelia: | 石川, 冬木 |
| Zdroj: | Journal of Biological Chemistry. 287:23440-23450 |
| Informácie o vydavateľovi: | Elsevier BV, 2012. |
| Rok vydania: | 2012 |
| Predmety: | 0301 basic medicine, Hot Temperature, Physiological/physiology, Cell Cycle Proteins, Oxidants/pharmacology, Gene Expression Regulation, Fungal, Histone chaperone, Physiological/genetics, Cross tolerance, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Nuclear Proteins, Fission yeast, Oxidants, Nucleosomes/metabolism, Adaptation, Physiological, Nucleosomes, Fungal, Schizosaccharomyces pombe Proteins/genetics, Drug, Histone Chaperones/physiology, Transcription, Hydrogen Peroxide/pharmacology, Schizosaccharomyces/drug effects, DNA Polymerase II/metabolism, Nuclear Proteins/physiology, Transcription Factors/genetics, Oxidative Stress/physiology, HIRA, Transcription Factors/physiology, Dose-Response Relationship, 03 medical and health sciences, Schizosaccharomyces pombe Proteins/physiology, Genetic, Schizosaccharomyces/genetics, Schizosaccharomyces, Humans, Nucleosomes/genetics, Histone Chaperones, Adaptation, Cell Cycle Proteins/genetics, Histone Chaperones/genetics, Schizosaccharomyces/growth & development, Dose-Response Relationship, Drug, Stress response, Gene Expression Profiling, DNA Polymerase II, Hydrogen Peroxide, Nuclear Proteins/genetics, Cell Cycle Proteins/physiology, Oxidative Stress, Gene Expression Regulation, Mutation, Schizosaccharomyces pombe Proteins, Transcription Factors |
| Popis: | Cells that have been pre-exposed to mild stress (priming stress) acquire transient resistance to subsequent severe stress even under different combinations of stresses. This phenomenon is called cross-tolerance. Although it has been reported that cross-tolerance occurs in many organisms, the molecular basis is not clear yet. Here, we identified slm9(+) as a responsible gene for the cross-tolerance in the fission yeast Schizosaccharomyces pombe. Slm9 is a homolog of mammalian HIRA histone chaperone. HIRA forms a conserved complex and gene disruption of other HIRA complex components, Hip1, Hip3, and Hip4, also yielded a cross-tolerance-defective phenotype, indicating that the fission yeast HIRA is involved in the cross-tolerance as a complex. We also revealed that Slm9 was recruited to the stress-responsive gene loci upon stress treatment in an Atf1-dependent manner. The expression of stress-responsive genes under stress conditions was compromised in HIRA disruptants. Consistent with this, Pol II recruitment and nucleosome eviction at these gene loci were impaired in slm9Δ cells. Furthermore, we found that the priming stress enhanced the expression of stress-responsive genes in wild-type cells that were exposed to the severe stress. These observations suggest that HIRA functions in stress response through transcriptional regulation. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m112.349944 |
| Prístupová URL adresa: | http://www.jbc.org/content/287/28/23440.full.pdf https://pubmed.ncbi.nlm.nih.gov/22589550 http://europepmc.org/articles/PMC3390621 https://www.jbc.org/article/S0021-9258(20)43354-X/fulltext https://www.sciencedirect.com/science/article/pii/S002192582043354X https://www.jbc.org/content/287/28/23440.full.pdf https://pubmed.ncbi.nlm.nih.gov/22589550/ http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/176347/1/jbc.M112.349944.pdf |
| Rights: | CC BY |
| Prístupové číslo: | edsair.doi.dedup.....51a89081dafece5cb4eb3a76f49b5d32 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Cells that have been pre-exposed to mild stress (priming stress) acquire transient resistance to subsequent severe stress even under different combinations of stresses. This phenomenon is called cross-tolerance. Although it has been reported that cross-tolerance occurs in many organisms, the molecular basis is not clear yet. Here, we identified slm9(+) as a responsible gene for the cross-tolerance in the fission yeast Schizosaccharomyces pombe. Slm9 is a homolog of mammalian HIRA histone chaperone. HIRA forms a conserved complex and gene disruption of other HIRA complex components, Hip1, Hip3, and Hip4, also yielded a cross-tolerance-defective phenotype, indicating that the fission yeast HIRA is involved in the cross-tolerance as a complex. We also revealed that Slm9 was recruited to the stress-responsive gene loci upon stress treatment in an Atf1-dependent manner. The expression of stress-responsive genes under stress conditions was compromised in HIRA disruptants. Consistent with this, Pol II recruitment and nucleosome eviction at these gene loci were impaired in slm9Δ cells. Furthermore, we found that the priming stress enhanced the expression of stress-responsive genes in wild-type cells that were exposed to the severe stress. These observations suggest that HIRA functions in stress response through transcriptional regulation. |
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| ISSN: | 00219258 |
| DOI: | 10.1074/jbc.m112.349944 |