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Phytochemical characterization and biological activities of Stenomesson miniatum bulb extract, a medicinal plant of the Andes

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Title: Phytochemical characterization and biological activities of Stenomesson miniatum bulb extract, a medicinal plant of the Andes
Authors: Mariacaterina Lianza, Carmela Fimognari, Jean-Marc Nuzillard, Ferruccio Poli, Jean-Hugues Renault, Francesca Bonvicini, Giulia Greco, Eleonora Turrini
Contributors: Alma Mater Studiorum Università di Bologna = University of Bologna (UNIBO), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Source: Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 158:182-189
Publisher Information: Informa UK Limited, 2023.
Publication Year: 2023
Subject Terms: Artefact, Amaryllidaceae alkaloids, Urceolina peruviana, Stenomesson miniatum, Andean traditional medicine, 13 C-NMR-based dereplication, 15. Life on land, 3. Good health, Jurkat cells, [CHIM]Chemical Sciences, A431 human epidermoid carcinoma, amaryllidaceae alkaloids, urceolina peruviana, C-13-NMR-based dereplication, jurkat cells, artefact, andean traditional medicine
Description: The fresh bulbs of Stenomesson miniatum, a plant belonging to Amaryllidaceae family with a poorly investigated phytochemical profile, were traditionally employed by Andean healers to treat tumors and abscesses. The aims of this study were to characterize the alkaloid extract from the bulbs of S. miniatum and test its cytotoxic and antibacterial potential. The alkaloid extract was characterized by dereplication using various techniques (CPC, NMR, UPLC-HRMS) and referring to in-home or online databases for spectroscopic data matching. Cytotoxic activities were evaluated on A431 human epidermoid carcinoma cells through a metabolic assay, and on Jurkat human acute T-leukemia cells through a cell-impermeant fluorescent nuclear probe. Antibacterial assays were carried out against Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes by using a standardized microdilution broth method 96-well plate. Eleven known Amaryllidaceae alkaloids were identified together with another compound determined as being an extraction artefact. The alkaloid extract showed good cytotoxic activity against both the tumor cell lines, reaching an IC50 of 3.3 µg/mL against A431 cells and of 10.9 µg/mL against Jurkat cells. The analysis of the fractions allowed the activity to be attributed to the presence of pretazettine and haemathamine. Conversely, no antibacterial activities were recorded for any of the samples.
Document Type: Article
File Description: application/pdf; application/zip
Language: English
ISSN: 1724-5575
1126-3504
DOI: 10.1080/11263504.2023.2293037
DOI: 10.20944/preprints202305.0921.v2
DOI: 10.5281/zenodo.4574016
Access URL: https://hdl.handle.net/11585/954209
https://doi.org/10.1080/11263504.2023.2293037
https://www.tandfonline.com/doi/full/10.1080/11263504.2023.2293037
Rights: CC BY
Accession Number: edsair.doi.dedup.....5194e8e1a8830549a84fc82b51b42396
Database: OpenAIRE
Description
Abstract:The fresh bulbs of Stenomesson miniatum, a plant belonging to Amaryllidaceae family with a poorly investigated phytochemical profile, were traditionally employed by Andean healers to treat tumors and abscesses. The aims of this study were to characterize the alkaloid extract from the bulbs of S. miniatum and test its cytotoxic and antibacterial potential. The alkaloid extract was characterized by dereplication using various techniques (CPC, NMR, UPLC-HRMS) and referring to in-home or online databases for spectroscopic data matching. Cytotoxic activities were evaluated on A431 human epidermoid carcinoma cells through a metabolic assay, and on Jurkat human acute T-leukemia cells through a cell-impermeant fluorescent nuclear probe. Antibacterial assays were carried out against Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes by using a standardized microdilution broth method 96-well plate. Eleven known Amaryllidaceae alkaloids were identified together with another compound determined as being an extraction artefact. The alkaloid extract showed good cytotoxic activity against both the tumor cell lines, reaching an IC50 of 3.3 µg/mL against A431 cells and of 10.9 µg/mL against Jurkat cells. The analysis of the fractions allowed the activity to be attributed to the presence of pretazettine and haemathamine. Conversely, no antibacterial activities were recorded for any of the samples.
ISSN:17245575
11263504
DOI:10.1080/11263504.2023.2293037