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Engineering Intercellular Communication using M13 Phagemid and CRISPR-based Gene Regulation for Multicellular Computing inEscherichia coli

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Bibliographic Details
Title: Engineering Intercellular Communication using M13 Phagemid and CRISPR-based Gene Regulation for Multicellular Computing inEscherichia coli
Authors: Hadiastri Kusumawardhani, Florian Zoppi, Roberto Avendaño, Yolanda Schaerli
Source: Nat Commun
Nature Communications, Vol 16, Iss 1, Pp 1-13 (2025)
Nature communications, vol. 16, no. 1, pp. 3569
Publisher Information: Cold Spring Harbor Laboratory, 2024.
Publication Year: 2024
Subject Terms: Computers, Molecular, Science, Escherichia coli, Synthetic Biology, Clustered Regularly Interspaced Short Palindromic Repeats, Cell Communication, Gene Expression Regulation, Bacterial, CRISPR-Cas Systems, RNA, Guide, CRISPR-Cas Systems, Genetic Engineering, Article, Escherichia coli/genetics, Escherichia coli/physiology, CRISPR-Cas Systems/genetics, RNA, Guide, CRISPR-Cas Systems/genetics, Cell Communication/genetics, Synthetic Biology/methods, Clustered Regularly Interspaced Short Palindromic Repeats/genetics, Genetic Engineering/methods
Description: Engineering multicellular consortia, where information processing is distributed across specialized cell types, offers a promising strategy for implementing sophisticated biocomputing systems. However, a major challenge remains in establishing orthogonal intercellular communication, or “wires”, within synthetic bacterial consortia. In this study, we address this bottleneck by integrating phagemid-mediated intercellular communication with CRISPR-based gene regulation for multicellular computing in syntheticE. coliconsortia. We achieve intercellular communication by regulating the transfer of single guide RNAs (sgRNAs) encoded on M13 phagemids from sender to receiver cells. Once inside the receiver cells, the transferred sgRNAs mediate gene regulation via CRISPR interference. Leveraging this approach, we successfully constructed one-, two-, and four-input logic gates. Our work expands the toolkit for intercellular communication and paves the way for complex information processing in synthetic microbial consortia, with diverse potential applications, including biocomputing, biosensing, and biomanufacturing.
Document Type: Article
Other literature type
File Description: application/pdf
ISSN: 2041-1723
DOI: 10.1101/2024.08.28.610043
DOI: 10.1038/s41467-025-58760-z
Access URL: https://pubmed.ncbi.nlm.nih.gov/40234414
https://doaj.org/article/dca625fb94fa428eaaad32cabfe37bb1
https://serval.unil.ch/resource/serval:BIB_203A4DFD1A98.P001/REF.pdf
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_203A4DFD1A986
https://serval.unil.ch/notice/serval:BIB_203A4DFD1A98
Rights: CC BY NC
CC BY
Accession Number: edsair.doi.dedup.....50707d0a2607b0281d18ed770582f5be
Database: OpenAIRE
Description
Abstract:Engineering multicellular consortia, where information processing is distributed across specialized cell types, offers a promising strategy for implementing sophisticated biocomputing systems. However, a major challenge remains in establishing orthogonal intercellular communication, or “wires”, within synthetic bacterial consortia. In this study, we address this bottleneck by integrating phagemid-mediated intercellular communication with CRISPR-based gene regulation for multicellular computing in syntheticE. coliconsortia. We achieve intercellular communication by regulating the transfer of single guide RNAs (sgRNAs) encoded on M13 phagemids from sender to receiver cells. Once inside the receiver cells, the transferred sgRNAs mediate gene regulation via CRISPR interference. Leveraging this approach, we successfully constructed one-, two-, and four-input logic gates. Our work expands the toolkit for intercellular communication and paves the way for complex information processing in synthetic microbial consortia, with diverse potential applications, including biocomputing, biosensing, and biomanufacturing.
ISSN:20411723
DOI:10.1101/2024.08.28.610043