In EDS ansehen

Bimodal expression of PHO84 is modulated by early termination of antisense transcription

Gespeichert in:
Bibliographische Detailangaben
Titel: Bimodal expression of PHO84 is modulated by early termination of antisense transcription
Autoren: Castelnuovo Manuele, Rahman Samir, Guffanti Elisa, Infantino Valentina, Stutz Françoise, Zenklusen Daniel
Quelle: Nat Struct Mol Biol
Nature Structural & Molecular Biology, Vol. 20, No 7 (2013) pp. 851-858
Nature structural & molecular biology
Verlagsinformationen: Springer Science and Business Media LLC, 2013.
Publikationsjahr: 2013
Schlagwörter: 0301 basic medicine, RNA Helicases/physiology, Nuclear Proteins/physiology, Transcription, Genetic, RNA, Antisense/genetics/metabolism, Saccharomyces cerevisiae, Polyadenylation, Article, Histone Deacetylases, Saccharomyces cerevisiae/genetics, 03 medical and health sciences, Proton-Phosphate Symporters, ddc:570, Histone-Lysine N-Methyltransferase/physiology, Gene Expression Regulation, Fungal, RNA, Antisense, Exosome Multienzyme Ribonuclease Complex/physiology, RNA, Messenger, Saccharomyces cerevisiae Proteins/biosynthesis/genetics/physiology, Promoter Regions, Genetic, In Situ Hybridization, Fluorescence, RNA, Fungal/genetics/metabolism, RNA, Messenger/biosynthesis/metabolism, 0303 health sciences, Proton-Phosphate Symporters/biosynthesis/genetics, Exosome Multienzyme Ribonuclease Complex, Models, Genetic, DNA Helicases/physiology, Polynucleotide Adenylyltransferase/physiology, DNA Helicases, Metalloendopeptidases, Nuclear Proteins, Polynucleotide Adenylyltransferase, RNA-Binding Proteins, RNA, Fungal, Histone-Lysine N-Methyltransferase, Promoter Regions, Genetic/genetics, Metalloendopeptidases/physiology, Multiprotein Complexes, Histone Deacetylases/physiology, RNA-Binding Proteins/physiology, RNA Helicases
Beschreibung: Many Saccharomyces cerevisiae genes encode antisense transcripts, some of which are unstable and degraded by the exosome component Rrp6. Loss of Rrp6 results in the accumulation of long PHO84 antisense (AS) RNAs and repression of sense transcription through PHO84 promoter deacetylation. We used single-molecule resolution fluorescent in situ hybridization (smFISH) to investigate antisense-mediated transcription regulation. We show that PHO84 AS RNA acts as a bimodal switch, in which continuous, low-frequency antisense transcription represses sense expression within individual cells. Surprisingly, antisense RNAs do not accumulate at the PHO84 gene but are exported to the cytoplasm. Furthermore, rather than stabilizing PHO84 AS RNA, the loss of Rrp6 favors its elongation by reducing early transcription termination by Nrd1-Nab3-Sen1. These observations suggest that PHO84 silencing results from antisense transcription through the promoter rather than the static accumulation of antisense RNAs at the repressed gene.
Publikationsart: Article
Other literature type
Dateibeschreibung: application/pdf
Sprache: English
ISSN: 1545-9985
1545-9993
DOI: 10.1038/nsmb.2598
Zugangs-URL: https://archive-ouverte.unige.ch/unige:36376/ATTACHMENT01
https://pubmed.ncbi.nlm.nih.gov/23770821
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972572/
https://archive-ouverte.unige.ch/unige:36376
https://core.ac.uk/display/60890853
http://europepmc.org/articles/PMC4972572
https://archive-ouverte.unige.ch/unige:36376/ATTACHMENT01
https://www.nature.com/articles/nsmb.2598.pdf
https://archive-ouverte.unige.ch/unige:36376
http://archive-ouverte.unige.ch/unige:36376
Rights: Springer TDM
Dokumentencode: edsair.doi.dedup.....4f6f07bfe571ed8f4dfbda6f58f186fa
Datenbank: OpenAIRE
Beschreibung
Abstract:Many Saccharomyces cerevisiae genes encode antisense transcripts, some of which are unstable and degraded by the exosome component Rrp6. Loss of Rrp6 results in the accumulation of long PHO84 antisense (AS) RNAs and repression of sense transcription through PHO84 promoter deacetylation. We used single-molecule resolution fluorescent in situ hybridization (smFISH) to investigate antisense-mediated transcription regulation. We show that PHO84 AS RNA acts as a bimodal switch, in which continuous, low-frequency antisense transcription represses sense expression within individual cells. Surprisingly, antisense RNAs do not accumulate at the PHO84 gene but are exported to the cytoplasm. Furthermore, rather than stabilizing PHO84 AS RNA, the loss of Rrp6 favors its elongation by reducing early transcription termination by Nrd1-Nab3-Sen1. These observations suggest that PHO84 silencing results from antisense transcription through the promoter rather than the static accumulation of antisense RNAs at the repressed gene.
ISSN:15459985
15459993
DOI:10.1038/nsmb.2598