Pattern of mineralization after regenerative periodontal therapy with enamel matrix proteins
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| Názov: | Pattern of mineralization after regenerative periodontal therapy with enamel matrix proteins |
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| Autori: | Sculean, A, Donos, N, Bosshardt, DD, Lang, NP |
| Zdroj: | European Journal of Oral Sciences, 114, 1, pp. 225-31 |
| Informácie o vydavateľovi: | Wiley, 2006. |
| Rok vydania: | 2006 |
| Predmety: | Periodontitis - Therapy, Phosphoproteins - Analysis, Periodontal Ligament, Tooth Root - Drug Effects - Ultrastructure, Sialoglycoproteins, 03 medical and health sciences, Calcification, Physiologic, 0302 clinical medicine, Dental Enamel Proteins, Microscopy, Electron, Transmission, Osteogenesis, UMCN 4.3: Tissue engineering and reconstructive surgery, Humans, Integrin-Binding Sialoprotein, Osteogenesis - Drug Effects, Cementogenesis, Tooth Root, Periodontitis, Dental Cementum - Drug Effects - Ultrastructure, Dental Cementum, Phosphoproteins, Immunohistochemistry, Dental Enamel Proteins - Therapeutic Use, Cementogenesis - Drug Effects, Sialoglycoproteins - Analysis, Guided Tissue Regeneration, Periodontal - Methods, Guided Tissue Regeneration, Periodontal, Periodontal Ligament - Drug Effects, Osteopontin, Calcification, Physiologic - Drug Effects |
| Popis: | A derivative (EMD) of enamel matrix proteins (EMPs) is used for periodontal regeneration because EMPs are believed to induce the formation of acellular extrinsic fiber cementum (AEFC). Other reports, however, indicate that EMPs have osteogenic potential. The aim of this study was to characterize the nature of the tissue that forms on the root surface following application of EMD. Ten human teeth affected by periodontitis and scheduled for extraction were treated with EMD. Four to six weeks later, they were extracted and processed for analysis by light microscopy and transmission electron microscopy. Immunocytochemistry with antibodies against bone sialoprotein (BSP) and osteopontin (OPN) was performed to determine the mineralization pattern. The newly formed tissues on the root were thick and contained embedded cells. Small mineralization foci were regularly seen, and large organic matrix patches were occasionally seen, but a distinct mineralization front was lacking. While labeling for BSP was always associated with small mineralization foci and large matrix patches, OPN labeling was seen inconsistently. It is concluded that tissues resembling either cellular intrinsic fiber cementum or a type of bone were observed. The mineralization pattern mostly resembled that found in bone, except for a few areas that exhibited a hitherto undescribed mineralization pattern. |
| Druh dokumentu: | Article Conference object |
| Jazyk: | English |
| ISSN: | 1600-0722 0909-8836 |
| DOI: | 10.1111/j.1600-0722.2006.00300.x |
| DOI: | 10.7892/boris.21593 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/16674690 https://hub.hku.hk/handle/10722/157955 https://repository.ubn.ru.nl/handle/2066/51058 https://boris.unibe.ch/21593/ https://core.ac.uk/display/37978496 https://www.narcis.nl/publication/RecordID/oai%3Arepository.ubn.ru.nl%3A2066%2F51058 https://www.onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0722.2006.00300.x http://hdl.handle.net/2066/51058 http://hdl.handle.net/10722/157955 |
| Rights: | Wiley Online Library User Agreement |
| Prístupové číslo: | edsair.doi.dedup.....4dea937a89b22c224d1680106a13af19 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | A derivative (EMD) of enamel matrix proteins (EMPs) is used for periodontal regeneration because EMPs are believed to induce the formation of acellular extrinsic fiber cementum (AEFC). Other reports, however, indicate that EMPs have osteogenic potential. The aim of this study was to characterize the nature of the tissue that forms on the root surface following application of EMD. Ten human teeth affected by periodontitis and scheduled for extraction were treated with EMD. Four to six weeks later, they were extracted and processed for analysis by light microscopy and transmission electron microscopy. Immunocytochemistry with antibodies against bone sialoprotein (BSP) and osteopontin (OPN) was performed to determine the mineralization pattern. The newly formed tissues on the root were thick and contained embedded cells. Small mineralization foci were regularly seen, and large organic matrix patches were occasionally seen, but a distinct mineralization front was lacking. While labeling for BSP was always associated with small mineralization foci and large matrix patches, OPN labeling was seen inconsistently. It is concluded that tissues resembling either cellular intrinsic fiber cementum or a type of bone were observed. The mineralization pattern mostly resembled that found in bone, except for a few areas that exhibited a hitherto undescribed mineralization pattern. |
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| ISSN: | 16000722 09098836 |
| DOI: | 10.1111/j.1600-0722.2006.00300.x |