Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats
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| Titel: | Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats |
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| Autoren: | Jessberger S, Clark RE, Broadbent NJ, Clemenson GD Jr, CONSIGLIO, Antonella, Lie DC, Squire LR, Gage F.H. |
| Weitere Verfasser: | Universitat de Barcelona |
| Quelle: | LEARN MEMORY r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu Fundació Sant Joan de Déu Recercat. Dipósit de la Recerca de Catalunya instname r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu Articles publicats en revistes (Patologia i Terapèutica Experimental) Dipòsit Digital de la UB Universidad de Barcelona |
| Verlagsinformationen: | Cold Spring Harbor Laboratory, 2009. |
| Publikationsjahr: | 2009 |
| Schlagwörter: | Male, 0301 basic medicine, Space perception, Hipocamp (Cervell), Percepció de l'espai, Genetic Vectors, Rats as laboratory animals, Neurones, Social Environment, Animals, Genetically Modified, Rats, Sprague-Dawley, Stereotaxic Techniques, Food Preferences, 03 medical and health sciences, Limbic system, 0302 clinical medicine, Memory, Animals, Maze Learning, Rates (Animals de laboratori), Neurons, Percepció de les formes, Lentivirus, Recognition, Psychology, Form perception, Rats, 3. Good health, Form Perception, Wnt Proteins, Sistema límbic, Space Perception, Dentate Gyrus, Hippocampus (Brain), Psychomotor Performance, Memòria |
| Beschreibung: | New granule cells are born throughout life in the dentate gyrus of the hippocampal formation. Given the fundamental role of the hippocampus in processes underlying certain forms of learning and memory, it has been speculated that newborn granule cells contribute to cognition. However, previous strategies aiming to causally link newborn neurons with hippocampal function used ablation strategies that were not exclusive to the hippocampus or that were associated with substantial side effects, such as inflammation. We here used a lentiviral approach to specifically block neurogenesis in the dentate gyrus of adult male rats by inhibiting WNT signaling, which is critically involved in the generation of newborn neurons, using a dominant-negative WNT (dnWNT). We found a level-dependent effect of adult neurogenesis on the long-term retention of spatial memory in the water maze task, as rats with substantially reduced levels of newborn neurons showed less preference for the target zone in probe trials >2 wk after acquisition compared with control rats. Furthermore, animals with strongly reduced levels of neurogenesis were impaired in a hippocampus-dependent object recognition task. Social transmission of food preference, a behavioral test that also depends on hippocampal function, was not affected by knockdown of neurogenesis. Here we identified a role for newborn neurons in distinct aspects of hippocampal function that will set the ground to further elucidate, using experimental and computational strategies, the mechanism by which newborn neurons contribute to behavior. |
| Publikationsart: | Article |
| Dateibeschreibung: | application/pdf |
| Sprache: | English |
| ISSN: | 1549-5485 1072-0502 |
| DOI: | 10.1101/lm.1172609 |
| Zugangs-URL: | http://learnmem.cshlp.org/content/16/2/147.full.pdf https://pubmed.ncbi.nlm.nih.gov/19181621 http://fundanet.fsjd.org/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12114 http://hdl.handle.net/2445/107102 https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12114 https://hdl.handle.net/2445/107102 http://diposit.ub.edu/dspace/handle/2445/107102 https://www.ncbi.nlm.nih.gov/pubmed/19181621 https://www.hifo.uzh.ch/research/jessberger/publications/Jessberger2009LM.pdf https://core.ac.uk/display/53600034 http://whoville.ucsd.edu/PDFs/446_Jessberger_etal_LearnMem_2009.pdf http://europepmc.org/articles/PMC2661246 https://hdl.handle.net/11379/33616 |
| Rights: | CC BY NC |
| Dokumentencode: | edsair.doi.dedup.....4d7f24dc69d7ca10aca910d5b43bd61c |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | New granule cells are born throughout life in the dentate gyrus of the hippocampal formation. Given the fundamental role of the hippocampus in processes underlying certain forms of learning and memory, it has been speculated that newborn granule cells contribute to cognition. However, previous strategies aiming to causally link newborn neurons with hippocampal function used ablation strategies that were not exclusive to the hippocampus or that were associated with substantial side effects, such as inflammation. We here used a lentiviral approach to specifically block neurogenesis in the dentate gyrus of adult male rats by inhibiting WNT signaling, which is critically involved in the generation of newborn neurons, using a dominant-negative WNT (dnWNT). We found a level-dependent effect of adult neurogenesis on the long-term retention of spatial memory in the water maze task, as rats with substantially reduced levels of newborn neurons showed less preference for the target zone in probe trials >2 wk after acquisition compared with control rats. Furthermore, animals with strongly reduced levels of neurogenesis were impaired in a hippocampus-dependent object recognition task. Social transmission of food preference, a behavioral test that also depends on hippocampal function, was not affected by knockdown of neurogenesis. Here we identified a role for newborn neurons in distinct aspects of hippocampal function that will set the ground to further elucidate, using experimental and computational strategies, the mechanism by which newborn neurons contribute to behavior. |
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| ISSN: | 15495485 10720502 |
| DOI: | 10.1101/lm.1172609 |