A comprehensive survey of the laminins and collagens type IV expressed in mouse Leydig cells and their regulation by LH/hCG
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| Názov: | A comprehensive survey of the laminins and collagens type IV expressed in mouse Leydig cells and their regulation by LH/hCG |
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| Autori: | Mazaud Guittot, Séverine, Vérot, Adélie, Odet, Fanny, Chauvin, Marie-Agnès, Le Magueresse-Battistoni, Brigitte |
| Prispievatelia: | Brébion, Alice, Communications Cellulaires et Différenciation (CCD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Régulations métaboliques, nutrition et diabètes - UM55 (RMND UM55), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Zdroj: | Reproduction. 135:479-488 |
| Informácie o vydavateľovi: | Bioscientifica, 2008. |
| Rok vydania: | 2008 |
| Predmety: | Male, 0301 basic medicine, Inbred Strains, Gene Expression, Chorionic Gonadotropin, Mice, MESH: Extracellular Matrix/metabolism, Protein Isoforms, MESH: Animals, MESH: Luteinizing Hormone/pharmacology, Cells, Cultured, MESH: Cells, Cultured, Reverse Transcriptase Polymerase Chain Reaction, Leydig Cells, MESH: Collagen Type IV/genetics, Extracellular Matrix, MESH: Chorionic Gonadotropin/pharmacology, Reverse Transcriptase Polymerase Chain Reaction/methods, MESH: Laminin/genetics, Extracellular Matrix/drug effects, Collagen Type IV, MESH: Gene Expression, Cells, MESH: Leydig Cells/drug effects, MESH: Reverse Transcriptase Polymerase Chain Reaction/methods, Mice, Inbred Strains, MESH: DNA Primers/genetics, Cell Line, 03 medical and health sciences, Protein Isoforms/genetics, Leydig Cells/metabolism, MESH: Leydig Cells/metabolism, Animals, Luteinizing Hormone/pharmacology, MESH: Mice, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, DNA Primers, DNA Primers/genetics, MESH: Protein Isoforms/genetics, Leydig Cells/drug effects, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Extracellular Matrix/metabolism, Luteinizing Hormone, MESH: Male, Laminin/genetics, MESH: Cell Line, Collagen Type IV/genetics, MESH: Extracellular Matrix/drug effects, Chorionic Gonadotropin/pharmacology, Laminin |
| Popis: | Extracellular matrix (ECM) proteins have been shown to alter Leydig cell steroidogenesis in vitro, substantiating the hypothesis that Leydig cell steroidogenic activity and matrix environment are interdependent events. However, the nature of the ECM components synthesized by Leydig cells and their regulation by LH/human chorionic gonadotropin (hCG) remain unknown. Here, we examine the occurrence of the 11 laminin subunits and the 6 alpha chains of collagen IV (COL4A1-6) by RT-PCR in Leydig cells cultured with or without LH/hCG. Leydig cells were a tumor Leydig cell line (mLTC-1) or 8-week-old mice Leydig cells. Based on PCR data, it is suggested that normal Leydig cells may synthesize a maximum of 11 laminin heterotrimers and the 6 alpha chains of collagen IV. They also may synthesize various proteases and inhibitors of the metzincin family. The mLTC-1 cells have a limited repertoire as compared with normal Leydig cells. Interestingly, none of the ten proteases and inhibitors monitored is under LH-hCG regulation whereas every protease and inhibitor of the serine protease family yet identified in Leydig cells is under gonadotropin regulation. In addition, a few laminin and collagen subunit genes are regulated by LH/hCG. These are laminins alpha3 and gamma3 (Lama3 and Lamc3), Col4a3, and Col4a6, which are negatively regulated by LH/hCG in both Leydig cell types, and Col4a4, which was downregulated in primary cultures but not in mLTC-1 cells. Collectively, the present study suggests that Leydig cells modulate in a selective fashion their matrix environment in response to their trophic hormone. This may alter the steroidogenic outcome of Leydig cells. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-07-0561 |
| Prístupová URL adresa: | https://rep.bioscientifica.com/downloadpdf/journals/rep/135/4/479.pdf https://pubmed.ncbi.nlm.nih.gov/18367508 https://www.ncbi.nlm.nih.gov/pubmed/18367508 https://rep.bioscientifica.com/downloadpdf/journals/rep/135/4/479.pdf https://pubmed.ncbi.nlm.nih.gov/18367508/ https://rep.bioscientifica.com/view/journals/rep/135/4/479.xml https://hal.science/hal-00671001v1 https://doi.org/10.1530/rep-07-0561 |
| Prístupové číslo: | edsair.doi.dedup.....4ca11ab7890b2ee0c2d65f704b3af960 |
| Databáza: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Extracellular matrix (ECM) proteins have been shown to alter Leydig cell steroidogenesis in vitro, substantiating the hypothesis that Leydig cell steroidogenic activity and matrix environment are interdependent events. However, the nature of the ECM components synthesized by Leydig cells and their regulation by LH/human chorionic gonadotropin (hCG) remain unknown. Here, we examine the occurrence of the 11 laminin subunits and the 6 alpha chains of collagen IV (COL4A1-6) by RT-PCR in Leydig cells cultured with or without LH/hCG. Leydig cells were a tumor Leydig cell line (mLTC-1) or 8-week-old mice Leydig cells. Based on PCR data, it is suggested that normal Leydig cells may synthesize a maximum of 11 laminin heterotrimers and the 6 alpha chains of collagen IV. They also may synthesize various proteases and inhibitors of the metzincin family. The mLTC-1 cells have a limited repertoire as compared with normal Leydig cells. Interestingly, none of the ten proteases and inhibitors monitored is under LH-hCG regulation whereas every protease and inhibitor of the serine protease family yet identified in Leydig cells is under gonadotropin regulation. In addition, a few laminin and collagen subunit genes are regulated by LH/hCG. These are laminins alpha3 and gamma3 (Lama3 and Lamc3), Col4a3, and Col4a6, which are negatively regulated by LH/hCG in both Leydig cell types, and Col4a4, which was downregulated in primary cultures but not in mLTC-1 cells. Collectively, the present study suggests that Leydig cells modulate in a selective fashion their matrix environment in response to their trophic hormone. This may alter the steroidogenic outcome of Leydig cells. |
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| ISSN: | 17417899 14701626 |
| DOI: | 10.1530/rep-07-0561 |