Colorectal cancer early methylation alterations affect the crosstalk between cell and surrounding environment, tracing a biomarker signature specific for this tumor
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| Titel: | Colorectal cancer early methylation alterations affect the crosstalk between cell and surrounding environment, tracing a biomarker signature specific for this tumor |
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| Autoren: | Fadda, Antonio, GENTILINI, ORESTE DAVIDE, MOI, LOREDANA, Barault, Ludovic, Leoni, Vera Piera, Sulas, Pia, Zorcolo, Luigi, Restivo, Angelo, Cabras, Francesco, Fortunato, Federica, Zavattari, Cesare, Varesco, Liliana, Gismondi, Viviana, De Miglio, Maria Rosaria, Scanu, Antonio Mario, Colombi, Federica, Lombardi, Pasquale, Sarotto, Ivana, Loi, Eleonora, Leone, Francesco, Giordano, Silvia, Di Nicolantonio, Federica, Columbano, Amedeo, Zavattari, Patrizia |
| Weitere Verfasser: | Fadda, Antonio, Gentilini, Davide, Moi, Loredana, Barault, Ludovic, Leoni, Vera Piera, Sulas, Pia, Zorcolo, Luigi, Restivo, Angelo, Cabras, Francesco, Fortunato, Federica, Zavattari, Cesare, Varesco, Liliana, Gismondi, Viviana, De Miglio, Maria Rosaria, Scanu, Antonio Mario, Colombi, Federica, Lombardi, Pasquale, Sarotto, Ivana, Loi, Eleonora, Leone, Francesco, Giordano, Silvia, Di Nicolantonio, Federica, Columbano, Amedeo, Zavattari, Patrizia |
| Quelle: | International Journal of Cancer. 143:907-920 |
| Verlagsinformationen: | Wiley, 2018. |
| Publikationsjahr: | 2018 |
| Schlagwörter: | Adenoma, Male, 0301 basic medicine, Down-Regulation, DNA Methylation, Middle Aged, 3. Good health, CRC, Gene Expression Regulation, Neoplastic, Feces, 03 medical and health sciences, Downregulation, Biomarkers, Tumor, Tumor biomarker, Methylome, Specificity and sensitivity, Humans, Computer Simulation, CpG Islands, Female, Neoplasm Metastasis, Colorectal Neoplasms, Signal Transduction |
| Beschreibung: | Colorectal cancer (CRC) develops through the accumulation of both genetic and epigenetic alterations. However, while the former are already used as prognostic and predictive biomarkers, the latter are less well characterized. Here, performing global methylation analysis on both CRCs and adenomas by Illumina Infinium HumanMethylation450 Bead Chips, we identified a panel of 74 altered CpG islands, demonstrating that the earliest methylation alterations affect genes coding for proteins involved in the crosstalk between cell and surrounding environment. The panel discriminates CRCs and adenomas from peritumoral and normal mucosa with very high specificity (100%) and sensitivity (99.9%). Interestingly, over 70% of the hypermethylated islands resulted in downregulation of gene expression. To establish the possible usefulness of these non‐invasive markers for detection of colon cancer, we selected three biomarkers and identified the presence of altered methylation in stool DNA and plasma cell‐free circulating DNA from CRC patients. |
| Publikationsart: | Article |
| Dateibeschreibung: | application/pdf; STAMPA |
| Sprache: | English |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.31380 |
| Zugangs-URL: | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijc.31380 https://pubmed.ncbi.nlm.nih.gov/29542109 https://moh-it.pure.elsevier.com/en/publications/colorectal-cancer-early-methylation-alterations-affect-the-crosst-2 https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=201802253959351258 https://europepmc.org/abstract/MED/29542109 https://pubmed.ncbi.nlm.nih.gov/29542109/ http://www.ncbi.nlm.nih.gov/pubmed/29542109 https://www.ncbi.nlm.nih.gov/pubmed/29542109 https://hdl.handle.net/20.500.11768/154838 https://doi.org/10.1002/ijc.31380 https://hdl.handle.net/11388/203273 https://iris.unito.it/retrieve/handle/2318/1662608/397218/Fadda_IJC_2018_postprint.pdf https://doi.org/10.1002/ijc.31380 https://hdl.handle.net/11584/242471 https://doi.org/10.1002/ijc.31380 https://hdl.handle.net/2318/1662608 https://doi.org/10.1002/ijc.31380 |
| Rights: | Wiley Online Library User Agreement |
| Dokumentencode: | edsair.doi.dedup.....4c28c0305bb1dda49d9a34e2a6bd6f96 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Colorectal cancer (CRC) develops through the accumulation of both genetic and epigenetic alterations. However, while the former are already used as prognostic and predictive biomarkers, the latter are less well characterized. Here, performing global methylation analysis on both CRCs and adenomas by Illumina Infinium HumanMethylation450 Bead Chips, we identified a panel of 74 altered CpG islands, demonstrating that the earliest methylation alterations affect genes coding for proteins involved in the crosstalk between cell and surrounding environment. The panel discriminates CRCs and adenomas from peritumoral and normal mucosa with very high specificity (100%) and sensitivity (99.9%). Interestingly, over 70% of the hypermethylated islands resulted in downregulation of gene expression. To establish the possible usefulness of these non‐invasive markers for detection of colon cancer, we selected three biomarkers and identified the presence of altered methylation in stool DNA and plasma cell‐free circulating DNA from CRC patients. |
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| ISSN: | 10970215 00207136 |
| DOI: | 10.1002/ijc.31380 |