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Osteointegration of functionalised high-performance oxide ceramics: imaging from micro-computed tomography

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Title: Osteointegration of functionalised high-performance oxide ceramics: imaging from micro-computed tomography
Authors: Filippo Migliorini, Jörg Eschweiler, Marcel Betsch, Nicola Maffulli, Markus Tingart, Frank Hildebrand, Sophie Lecouturier, Björn Rath, Hanno Schenker
Source: J Orthop Surg Res
Journal of Orthopaedic Surgery and Research, Vol 19, Iss 1, Pp 1-8 (2024)
Journal of orthopaedic surgery and research 19, 411 (2024). doi:10.1186/s13018-024-04918-2
Publisher Information: Springer Science and Business Media LLC, 2024.
Publication Year: 2024
Subject Terms: Micro-CT, Orthopedic surgery, 0301 basic medicine, Ceramics, 0303 health sciences, Bone Morphogenetic Protein 2, Oxides, Diseases of the musculoskeletal system, X-Ray Microtomography, Ossification, Osteointegration, 03 medical and health sciences, RC925-935, Osseointegration, Animals, Female [MeSH], Bone Morphogenetic Protein 2 [MeSH], Ceramics [MeSH], Animals [MeSH], Oxides [MeSH], Implants, X-Ray Microtomography/methods [MeSH], Osseointegration/physiology [MeSH], Rabbits [MeSH], Oligopeptides [MeSH], Research Article, Female, Rabbits, Oligopeptides, RD701-811
Description: Background This study evaluated the osseointegration potential of functionalised high-performance oxide ceramics (HPOC) in isolation or coated with BMP-2 or RGD peptides in 36 New Zeeland female rabbits using micro-computed tomography (micro CT). The primary outcomes of interest were to assess the amount of ossification evaluating the improvement in the bone volume/ total volume (BV/TV) ratio and trabecular thickness at 6 and 12 weeks. The second outcome of interest was to investigate possible differences in osteointegration between the functionalised silanised HPOC in isolation or coated with Bone Morphogenetic Protein 2 (BMP-2) or RGD peptides. Methods 36 adult female New Zealand white rabbits with a minimum weight of three kg were used. One-third of HPOCs were functionalised with silicon suboxide (SiOx), a third with BMP-2 (sHPOC-BMP2), and another third with RGD (sHPOC-RGD). All samples were scanned with a high-resolution micro CT (U-CTHR, MILabs B.V., Houten, The Netherlands) with a reconstructed voxel resolution of 10 µm. MicroCT scans were reconstructed in three planes and processed using Imalytics Preclinical version 2.1 (Gremse-IT GmbH, Aachen, Germany) software. The total volume (TV), bone volume (BV) and ratio BV/TV were calculated within the coating area. Results BV/TV increased significantly from 6 to 12 weeks in all HPOCs: silanised (P = 0.01), BMP-2 (P Conclusion Sinalised HPOC in isolation or functionalised with BMP-2 or RGD promotes in vivo osteointegration. The sinalised HOPC functionalised with BMP-2 demonstrated the greatest osseointegration.
Document Type: Article
Other literature type
Language: English
ISSN: 1749-799X
DOI: 10.1186/s13018-024-04918-2
DOI: 10.18154/rwth-conv-254238
Access URL: https://pubmed.ncbi.nlm.nih.gov/39026349
https://doaj.org/article/b8e4c7afa7974f8a91d2dddc82a8f7a1
https://repository.publisso.de/resource/frl:6502756
https://publications.rwth-aachen.de/record/1007882
Rights: CC BY
URL: http://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (http://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Accession Number: edsair.doi.dedup.....49c1ca99bdddda9c8e55c88f11df87e0
Database: OpenAIRE
Description
Abstract:Background This study evaluated the osseointegration potential of functionalised high-performance oxide ceramics (HPOC) in isolation or coated with BMP-2 or RGD peptides in 36 New Zeeland female rabbits using micro-computed tomography (micro CT). The primary outcomes of interest were to assess the amount of ossification evaluating the improvement in the bone volume/ total volume (BV/TV) ratio and trabecular thickness at 6 and 12 weeks. The second outcome of interest was to investigate possible differences in osteointegration between the functionalised silanised HPOC in isolation or coated with Bone Morphogenetic Protein 2 (BMP-2) or RGD peptides. Methods 36 adult female New Zealand white rabbits with a minimum weight of three kg were used. One-third of HPOCs were functionalised with silicon suboxide (SiOx), a third with BMP-2 (sHPOC-BMP2), and another third with RGD (sHPOC-RGD). All samples were scanned with a high-resolution micro CT (U-CTHR, MILabs B.V., Houten, The Netherlands) with a reconstructed voxel resolution of 10 µm. MicroCT scans were reconstructed in three planes and processed using Imalytics Preclinical version 2.1 (Gremse-IT GmbH, Aachen, Germany) software. The total volume (TV), bone volume (BV) and ratio BV/TV were calculated within the coating area. Results BV/TV increased significantly from 6 to 12 weeks in all HPOCs: silanised (P = 0.01), BMP-2 (P Conclusion Sinalised HPOC in isolation or functionalised with BMP-2 or RGD promotes in vivo osteointegration. The sinalised HOPC functionalised with BMP-2 demonstrated the greatest osseointegration.
ISSN:1749799X
DOI:10.1186/s13018-024-04918-2