The effect of aging on the bone healing properties of blood plasma
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| Název: | The effect of aging on the bone healing properties of blood plasma |
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| Autoři: | Faez Saleh Al-Hamed, Rania Rodan, Jose Luis Ramirez-Garcialuna, Osama Elkashty, Nasser Al-Shahrani, Simon D Tran, Marie Lordkipanidzé, Mari Kaartinen, Zahi Badran, Faleh Tamimi |
| Zdroj: | Injury. 52:1697-1708 |
| Informace o vydavateli: | Elsevier BV, 2021. |
| Rok vydání: | 2021 |
| Témata: | collagen, 0301 basic medicine, Aging, Bone Regeneration, cell infiltration, bone volume, GM-CSF signaling, wound healing, immune response, Plasma, iron, bone regeneration, rat, animal, phosphorus, micro-computed tomography, mass spectrometry, 0303 health sciences, elemental analysis, bioinformatics, nucleotide, fracture healing, mass fragmentography, metabolomics, aged, female, protein expression level, histopathology, metabolome, morphometry, signal transduction, granulocyte macrophage colony stimulating factor, transcription factor RUNX2, cross linking, spectroscopy, bone marrow, inductively coupled plasma optical emission spectrometry, proteome, animal experiment, concentration (parameter), macrophage, interleukin 7, lymphocyte, electrospray mass spectrometry, Article, animal tissue, 03 medical and health sciences, proteomics, interleukin 20, Animals, controlled study, liquid chromatography-mass spectrometry, plasma, pentose phosphate cycle, bone defect, data analysis software, Wound Healing, nonhuman, Tibia, animal model, aging, Rats, thrombocyte, inflammation, citric acid cycle, collagen fibril, mast cell, interleukin 15, tibia, upregulation |
| Popis: | Age-related changes in blood composition have been found to affect overall health. Thus, this study aimed to understand the effect of these changes on bone healing by assessing how plasma derived from young and old rats affect bone healing using a rat model.. Blood plasma was collected from 6-month and 24-month old rats. Differences in elemental composition and metabolome were assessed using optical emission spectrometry and liquid mass spectrometry, respectively. Bilateral tibial bone defects were created in eight rats. Young plasma was randomly applied to one defect, while aged plasma was applied to the contralateral one. Rats were euthanized after two weeks, and their tibiae were analyzed using micro-CT and histology. The proteome of bone marrow was analyzed in an additional group of three rats.Bone-defects treated with aged-plasma were significantly bigger in size and presented lower bone volume/tissue volume compared to defects treated with young-plasma. Histomorphometric analysis showed fewer mast cells, macrophages, and lymphocytes in defects treated with old versus young plasma. The proteome analysis showed that young plasma upregulated pathways required for bone healing (e.g. RUNX2, platelet signaling, and crosslinking of collagen fibrils) whereas old plasma upregulated pathways, involved in disease and inflammation (e.g. IL-7, IL-15, IL-20, and GM-CSF signaling). Plasma derived from old rats presented higher concentrations of iron, phosphorous, and nucleotide metabolites as well as lower concentrations of platelets, citric acid cycle, and pentose phosphate pathway metabolites compared to plasma derived from young rats.bone defects treated with plasma-derived from young rats showed better healing compared to defects treated with plasma-derived from old rats. The application of young and old plasmas has different effects on the proteome of bone defects. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0020-1383 |
| DOI: | 10.1016/j.injury.2021.05.001 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/34049703 https://www.sciencedirect.com/science/article/pii/S0020138321004265 https://europepmc.org/article/MED/34049703 https://hdl.handle.net/10576/31817 |
| Rights: | Elsevier TDM |
| Přístupové číslo: | edsair.doi.dedup.....48bbe96e1ac44f246677cba8cb96d952 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Age-related changes in blood composition have been found to affect overall health. Thus, this study aimed to understand the effect of these changes on bone healing by assessing how plasma derived from young and old rats affect bone healing using a rat model.. Blood plasma was collected from 6-month and 24-month old rats. Differences in elemental composition and metabolome were assessed using optical emission spectrometry and liquid mass spectrometry, respectively. Bilateral tibial bone defects were created in eight rats. Young plasma was randomly applied to one defect, while aged plasma was applied to the contralateral one. Rats were euthanized after two weeks, and their tibiae were analyzed using micro-CT and histology. The proteome of bone marrow was analyzed in an additional group of three rats.Bone-defects treated with aged-plasma were significantly bigger in size and presented lower bone volume/tissue volume compared to defects treated with young-plasma. Histomorphometric analysis showed fewer mast cells, macrophages, and lymphocytes in defects treated with old versus young plasma. The proteome analysis showed that young plasma upregulated pathways required for bone healing (e.g. RUNX2, platelet signaling, and crosslinking of collagen fibrils) whereas old plasma upregulated pathways, involved in disease and inflammation (e.g. IL-7, IL-15, IL-20, and GM-CSF signaling). Plasma derived from old rats presented higher concentrations of iron, phosphorous, and nucleotide metabolites as well as lower concentrations of platelets, citric acid cycle, and pentose phosphate pathway metabolites compared to plasma derived from young rats.bone defects treated with plasma-derived from young rats showed better healing compared to defects treated with plasma-derived from old rats. The application of young and old plasmas has different effects on the proteome of bone defects. |
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| ISSN: | 00201383 |
| DOI: | 10.1016/j.injury.2021.05.001 |