Histology of tendon and enthesis – suitable techniques for specific research questions
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| Titel: | Histology of tendon and enthesis – suitable techniques for specific research questions |
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| Autoren: | N Angrisani, E Willbold, A Kampmann, A Derksen, J Reifenrath |
| Quelle: | European Cells & Materials, Vol 43, Pp 228-251 (2022) |
| Verlagsinformationen: | Forum Multimedia Publishing LLC, 2022. |
| Publikationsjahr: | 2022 |
| Schlagwörter: | Orthopedic surgery, 0301 basic medicine, Rupture, staining techniques, 0303 health sciences, Sheep, hard tissue sectioning, Histological Techniques, histological evaluation, Diseases of the musculoskeletal system, Prostheses and Implants, Rats, 3. Good health, Tendons, 03 medical and health sciences, Rotator Cuff, RC925-935, Animals, RD701-811 |
| Beschreibung: | he musculoskeletal system consists of different components comprising a wide range of tissue types, with tendons being one part. Tendon degeneration or rupture have a high prevalence in all age groups, often with poor outcomes of surgical treatment such as chronic pain and high re-tear rates. Therefore, much effort has been directed to further develop diagnostic and therapeutic methods as well as reconstruction techniques, including using adequate placeholders or implants. Diagnostic approaches and advanced stages of preclinical studies will inevitably include histological examination of the pathologically affected tissue. The present study presents adequate tendon-related, histological techniques, including the embedding of soft- and hard-tissue samples in different media. Consideration is also given to samples containing residual implant materials or having been subjected to standard staining protocols and immunohistochemical procedures. The study further examines cells and tendon structure to detect degenerative, fibrotic or inflammatory conditions and possible foreign-body responses to implanted materials. Infraspinatus tendons from preclinical studies carried on rat and sheep samples, as well as human biceps tendon samples, have been used as example materials. |
| Publikationsart: | Article |
| DOI: | 10.22203/ecm.v043a16 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/35608562 https://doaj.org/article/c3449ea68a774091b0c0eec2955b9304 |
| Dokumentencode: | edsair.doi.dedup.....43b8135df3002a9fe06c04d822f9f74c |
| Datenbank: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | he musculoskeletal system consists of different components comprising a wide range of tissue types, with tendons being one part. Tendon degeneration or rupture have a high prevalence in all age groups, often with poor outcomes of surgical treatment such as chronic pain and high re-tear rates. Therefore, much effort has been directed to further develop diagnostic and therapeutic methods as well as reconstruction techniques, including using adequate placeholders or implants. Diagnostic approaches and advanced stages of preclinical studies will inevitably include histological examination of the pathologically affected tissue. The present study presents adequate tendon-related, histological techniques, including the embedding of soft- and hard-tissue samples in different media. Consideration is also given to samples containing residual implant materials or having been subjected to standard staining protocols and immunohistochemical procedures. The study further examines cells and tendon structure to detect degenerative, fibrotic or inflammatory conditions and possible foreign-body responses to implanted materials. Infraspinatus tendons from preclinical studies carried on rat and sheep samples, as well as human biceps tendon samples, have been used as example materials. |
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| DOI: | 10.22203/ecm.v043a16 |