Molecular beacon-based bioimaging of multiple microRNAs during myogenesis
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| Názov: | Molecular beacon-based bioimaging of multiple microRNAs during myogenesis |
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| Autori: | Ye Lim Cho, Soonhag Kim, Ju Ri Chae, Kyung-Ju Choi, Won Jun Kang, Jong Doo Lee |
| Prispievatelia: | Won Jun Kang, Ye Lim Cho, Ju Ri Chae, Jong Doo Lee, Kyung-Ju Choi, Soonhag Kim, Kang, Won Jun, Lee, Jong Doo |
| Zdroj: | Biomaterials. 32:1915-1922 |
| Informácie o vydavateľovi: | Elsevier BV, 2011. |
| Rok vydania: | 2011 |
| Predmety: | 0301 basic medicine, Cell Differentiation/genetics, Blotting, Western, Muscle Development, Imaging, Cell Line, 03 medical and health sciences, Cell Differentiation/physiology, Animals, Humans, Molecular beacon, Microscopy, 0303 health sciences, Microscopy, Confocal, Blotting, Reverse Transcriptase Polymerase Chain Reaction, Myogenesis, MicroRNA, Cell Differentiation, MicroRNAs/genetics, 3. Good health, MicroRNAs, Confocal, Muscle Development/genetics, Muscle Development/physiology, Western, Multiple, HeLa Cells |
| Popis: | MicroRNAs (miRNAs, miR) are associated with multiple cellular processes and diseases. Here, we designed fluorescent DNA probes composed of stem loop-structured DNA complementary to miRNAs and fluorophore-quencher pairs [molecular beacon (MB)] to simultaneously monitor the biogenesis of miR-206 and miR-26a, which are highly expressed during myogenic differentiation. C2C12 cells were transfected with an MB targeting miR-26a and containing a 6-FAM-BHQ1 pair (miRNA-26a MB) or an MB targeting miR-206 with a Texas Red-BHQ2 pair (miRNA-206 MB). In vitro and in vivo fluorescence analysis revealed that, only in differentiated single C2C12 cell, significantly increased fluorescence signals of miRNA-26a MB, miRNA-206 MB or simultaneous incubation of both beacons were detected due to the hybridization of miR-206 or miR-26a with their respective beacons, resulting in activation of fluorescence. Our MB-based miRNA imaging system may serve as a new imaging probe for monitoring multiple miRNAs during various cellular or disease processes associated with miRNAs. |
| Druh dokumentu: | Article |
| Popis súboru: | 1915~1922 |
| Jazyk: | English |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2010.11.007 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/21122913 https://www.ncbi.nlm.nih.gov/pubmed/21122913 https://www.sciencedirect.com/science/article/pii/S0142961210014298 https://ir.ymlib.yonsei.ac.kr/handle/22282913/92965 http://www.sciencedirect.com/science/article/pii/S0142961210014298 https://pubmed.ncbi.nlm.nih.gov/21122913/ |
| Rights: | Elsevier TDM CC BY NC ND |
| Prístupové číslo: | edsair.doi.dedup.....4312e21c02de4615c98153d618b00cb6 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | MicroRNAs (miRNAs, miR) are associated with multiple cellular processes and diseases. Here, we designed fluorescent DNA probes composed of stem loop-structured DNA complementary to miRNAs and fluorophore-quencher pairs [molecular beacon (MB)] to simultaneously monitor the biogenesis of miR-206 and miR-26a, which are highly expressed during myogenic differentiation. C2C12 cells were transfected with an MB targeting miR-26a and containing a 6-FAM-BHQ1 pair (miRNA-26a MB) or an MB targeting miR-206 with a Texas Red-BHQ2 pair (miRNA-206 MB). In vitro and in vivo fluorescence analysis revealed that, only in differentiated single C2C12 cell, significantly increased fluorescence signals of miRNA-26a MB, miRNA-206 MB or simultaneous incubation of both beacons were detected due to the hybridization of miR-206 or miR-26a with their respective beacons, resulting in activation of fluorescence. Our MB-based miRNA imaging system may serve as a new imaging probe for monitoring multiple miRNAs during various cellular or disease processes associated with miRNAs. |
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| ISSN: | 01429612 |
| DOI: | 10.1016/j.biomaterials.2010.11.007 |