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Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments

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Bibliographic Details
Title: Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments
Authors: Balazs, D. J., Triandafillu, K., Wood, Paul, Chevolot, Y., Van Delden, Christian, Harms, H., Hollenstein, C., Mathieu, H. J.
Source: Biomaterials, Vol. 25, No 11 (2004) pp. 2139-2151
Publisher Information: Elsevier BV, 2004.
Publication Year: 2004
Subject Terms: Hot Temperature, Silver, Cross Infection/etiology/prevention & control, Cell Division/drug effects, Pseudomonas/ cytology/drug effects/physiology, Radio Waves, Surface Properties, 02 engineering and technology, 01 natural sciences, Bacterial Adhesion, Polyvinyl Chloride/ chemistry, Coated Materials, Biocompatible, Pseudomonas, Silver/chemistry/ pharmacology, Intubation, Intratracheal, Biofilms/drug effects/growth & development, Intubation, Intratracheal/adverse effects/ instrumentation, Humans, Sodium Hydroxide, Pseudomonas Infections, Polyvinyl Chloride, Coated Materials, Biocompatible/ chemical synthesis/pharmacology, Equipment Contamination/ prevention & control, ddc:616, Cross Infection, Sodium Hydroxide/chemistry, Pseudomonas Infections/etiology/prevention & control, 0104 chemical sciences, Equipment Failure Analysis, Biofilms, Bacterial Adhesion/ drug effects, Equipment Contamination, 0210 nano-technology, Cell Division
Description: Medical-grade poly(vinyl chloride) (PVC) was chemically modified to study how the incorporation of monovalent silver influences Pseudomonas aeruginosa adhesion and colonization. The modification investigated consisted of a radio frequency-oxygen (RF-O(2)) glow discharge pre-functionalization, followed by a two-step wet-treatment in sodium hydroxide and silver nitrate solutions. X-ray photoelectron spectroscopy (XPS) analysis and contact angle measurements were used to investigate the chemical nature and surface wettability of the films following each step of the modification. XPS analysis proved that the RF-O(2) plasma pre-functionalization of native PVC reproducibly increased the amount of functional groups representative of PVC additives, including ether/alcohol, esters and carboxyl groups. More specifically, we demonstrated that the O-C=O groups representative of the phthalic ester and zinc carboxylate additives identified for native PVC increased by two-fold following the RF-O(2) plasma pre-functionalization step. Although RF-O(2) pre-functionalization did not have an effect on the silver content of the NaOH/AgNO(3) treated substrates, such a modification was necessary for biomaterial products that did not have reproducible surfaces amongst production lots. XPS analysis also demonstrated that saponification with sodium hydroxide (NaOH) of esters, like those of the phthalic ester additives of PVC is a simple, irreversible method of hydrolysis, which produced sodium carboxylate and sodium phthalate salts. Exposure of native PVC to NaOH resulted in an increased surface hydrophilicity (from ca 90 degrees to ca 60 degrees ) due to dechlorination. XPS analysis following further incubation in silver nitrate demonstrated that silver ions can be trapped when the sodium of sodium carboxylate is replaced by silver after performing a second treatment with a monovalent silver-containing solution. The creation of silver salt on native PVC resulted in an ultra-hydrophobic (>120 degrees ) surface. The chemical modifications using NaOH and AgNO(3) wet treatments completely inhibited bacterial adhesion of four strains of P. aeruginosa to both native and oxygen-pre-functionalized PVC, and efficiently prevented colonization over longer periods (72 h). Our results suggest that surface modifications that incorporate silver ions would be extremely effective at reducing bacterial colonization to medical devices.
Document Type: Article
File Description: application/pdf
Language: English
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2003.08.053
Access URL: https://pubmed.ncbi.nlm.nih.gov/14741629
https://www.ncbi.nlm.nih.gov/pubmed/14741629
https://europepmc.org/article/MED/14741629
https://archive-ouverte.unige.ch/unige:7090
https://infoscience.epfl.ch/record/120157
https://www.sciencedirect.com/science/article/pii/S0142961203007117
http://www.sciencedirect.com/science/article/pii/S0142961203007117
https://archive-ouverte.unige.ch/unige:7090
Rights: Elsevier TDM
Accession Number: edsair.doi.dedup.....42e6510875e3cc6b4830086a4be46f67
Database: OpenAIRE
Description
Abstract:Medical-grade poly(vinyl chloride) (PVC) was chemically modified to study how the incorporation of monovalent silver influences Pseudomonas aeruginosa adhesion and colonization. The modification investigated consisted of a radio frequency-oxygen (RF-O(2)) glow discharge pre-functionalization, followed by a two-step wet-treatment in sodium hydroxide and silver nitrate solutions. X-ray photoelectron spectroscopy (XPS) analysis and contact angle measurements were used to investigate the chemical nature and surface wettability of the films following each step of the modification. XPS analysis proved that the RF-O(2) plasma pre-functionalization of native PVC reproducibly increased the amount of functional groups representative of PVC additives, including ether/alcohol, esters and carboxyl groups. More specifically, we demonstrated that the O-C=O groups representative of the phthalic ester and zinc carboxylate additives identified for native PVC increased by two-fold following the RF-O(2) plasma pre-functionalization step. Although RF-O(2) pre-functionalization did not have an effect on the silver content of the NaOH/AgNO(3) treated substrates, such a modification was necessary for biomaterial products that did not have reproducible surfaces amongst production lots. XPS analysis also demonstrated that saponification with sodium hydroxide (NaOH) of esters, like those of the phthalic ester additives of PVC is a simple, irreversible method of hydrolysis, which produced sodium carboxylate and sodium phthalate salts. Exposure of native PVC to NaOH resulted in an increased surface hydrophilicity (from ca 90 degrees to ca 60 degrees ) due to dechlorination. XPS analysis following further incubation in silver nitrate demonstrated that silver ions can be trapped when the sodium of sodium carboxylate is replaced by silver after performing a second treatment with a monovalent silver-containing solution. The creation of silver salt on native PVC resulted in an ultra-hydrophobic (>120 degrees ) surface. The chemical modifications using NaOH and AgNO(3) wet treatments completely inhibited bacterial adhesion of four strains of P. aeruginosa to both native and oxygen-pre-functionalized PVC, and efficiently prevented colonization over longer periods (72 h). Our results suggest that surface modifications that incorporate silver ions would be extremely effective at reducing bacterial colonization to medical devices.
ISSN:01429612
DOI:10.1016/j.biomaterials.2003.08.053