New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
Saved in:
| Title: | New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria |
|---|---|
| Authors: | Beatriz Altamira-Algarra, Estel Rueda, Artai Lage, David San León, Juan F. Martínez-Blanch, Juan Nogales, Joan García, Eva Gonzalez-Flo |
| Contributors: | Ministerio de Universidades (España), San León, David [0000-0001-8138-500X], Nogales, Juan [0000-0002-4961-0833], Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72], Universitat Politècnica de Catalunya. Doctorat en Enginyeria Ambiental, Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental, Universitat Politècnica de Catalunya. GEMMA - Grup d'Enginyeria i Microbiologia del Medi Ambient |
| Source: | UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Digital.CSIC. Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) New Biotechnology |
| Publisher Information: | Elsevier BV, 2023. |
| Publication Year: | 2023 |
| Subject Terms: | 16 S rRNA gene high-throughput sequencing, Biopolímers, Polyesters, Hydroxybutyrates, Extracellular polysaccharide, Acetates, Polyhydroxyalkanoate, Cyanobacteria, Carbon, Photosynthethic Microbiome, Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental, Biopolymers |
| Description: | Seven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutirate (PHB) and exopolysaccharides (EPS) production, two alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and optimizing the effect of three parameters (organic and inorganic carbon and days under light:dark cycles) by Box-Behnken design. Results showed that optimal conditions for both biopolymers synthesis were microbiome-dependent; however, the addition of organic carbon boosted PHB production in all the tested microbiomes, producing up to 14%dcwPHB with the addition of 1.2 g acetate·L-1and seven days under light:dark photoperiods. The highest EPS production was 59 mg·L-1with the addition of 1.2 g acetate·L-1and four days under light:dark photoperiods. The methodology used in this article is suitable for enriching microbiomes in cyanobacteria, and for testing the best conditions for bioproducts synthesis for further scale up. |
| Document Type: | Article |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1871-6784 |
| DOI: | 10.1016/j.nbt.2023.10.008 |
| DOI: | 10.1101/2023.05.30.542819 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/37852438 http://hdl.handle.net/10261/363672 https://api.elsevier.com/content/abstract/scopus_id/85175199556 https://hdl.handle.net/2117/404043 https://doi.org/10.1016/j.nbt.2023.10.008 |
| Rights: | CC BY Elsevier TDM |
| Accession Number: | edsair.doi.dedup.....3cb409dd871d5acd44bcf914f6fe4d36 |
| Database: | OpenAIRE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstract: | Seven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutirate (PHB) and exopolysaccharides (EPS) production, two alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and optimizing the effect of three parameters (organic and inorganic carbon and days under light:dark cycles) by Box-Behnken design. Results showed that optimal conditions for both biopolymers synthesis were microbiome-dependent; however, the addition of organic carbon boosted PHB production in all the tested microbiomes, producing up to 14%dcwPHB with the addition of 1.2 g acetate·L-1and seven days under light:dark photoperiods. The highest EPS production was 59 mg·L-1with the addition of 1.2 g acetate·L-1and four days under light:dark photoperiods. The methodology used in this article is suitable for enriching microbiomes in cyanobacteria, and for testing the best conditions for bioproducts synthesis for further scale up. |
|---|---|
| ISSN: | 18716784 |
| DOI: | 10.1016/j.nbt.2023.10.008 |