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Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection

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Název: Type 1 and type 2 cytokine gene expression by viral gp135 surface protein-activated T lymphocytes in caprine arthritis-encephalitis lentivirus infection
Autoři: Cheevers, W P, Beyer, J C, Knowles, D P
Zdroj: Journal of Virology. 71:6259-6263
Informace o vydavateli: American Society for Microbiology, 1997.
Rok vydání: 1997
Témata: CD4-Positive T-Lymphocytes, 0301 basic medicine, T-Lymphocytes - immunology, Arthritis-Encephalitis Virus, Caprine, Lentivirus Infections - immunology, T-Lymphocytes, Lymphocyte Activation, Caprine, Interleukin-4 - genetics, Interleukin-2 - genetics, Interferon-gamma, 03 medical and health sciences, Interferon-gamma - genetics, Viral Envelope Proteins, Animals, RNA, Messenger, Viral Envelope Proteins - physiology, 0303 health sciences, Goats, 3. Good health, Messenger - analysis, Arthritis-Encephalitis Virus, Lentivirus Infections, RNA, Interleukin-2, Interleukin-4
Popis: Peripheral blood mononuclear cells (PBMC) from Saanen goats experimentally infected with the lentivirus caprine arthritis-encephalitis virus (CAEV) were evaluated by semiquantitative reverse transcriptase PCR for gamma interferon (IFN-gamma), interleukin-4 (IL-4), and IL-2 gene expression following in vitro stimulation with purified CAEV gp135 surface protein (SU). Studies examined three goats with chronic arthritis and four clinically asymptomatic goats at 5 years postinfection. SU-responsive IFN-gamma mRNA-positive cells and IL-4 mRNA-positive cells in PBMC from infected goats reflected differences in lymphokine balance associated with disease status. IFN-gamma mRNA-positive cells were dominant in PBMC from asymptomatic goats, whereas SU-responsive IL-4 mRNA-positive cells were dominant in PBMC from goats with arthritis. IL-2 gene expression was not responsive to SU stimulation of PBMC from either asymptomatic or arthritic goats. Lymphokine mRNA profiles in SU-stimulated PBMC were dependent on the presence of CD4+ T lymphocytes. The results indicate that asymptomatic goats have a dominant population of CAEV SU-reactive T-helper 1 (Th1)-like lymphocytes in PBMC whereas goats with clinical arthritis have a dominant population of SU-reactive Th2-like lymphocytes.
Druh dokumentu: Article
Jazyk: English
ISSN: 1098-5514
0022-538X
DOI: 10.1128/jvi.71.8.6259-6263.1997
Přístupová URL adresa: https://jvi.asm.org/content/jvi/71/8/6259.full.pdf
https://pubmed.ncbi.nlm.nih.gov/9223529
https://jvi.asm.org/content/71/8/6259.short
http://europepmc.org/articles/PMC191895
https://jvi.asm.org/content/71/8/6259.full.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC191895/
Rights: ASM Journals Non-Commercial TDM
Přístupové číslo: edsair.doi.dedup.....3c79461ab406ce92ba1690afceacf27a
Databáze: OpenAIRE
Popis
Abstrakt:Peripheral blood mononuclear cells (PBMC) from Saanen goats experimentally infected with the lentivirus caprine arthritis-encephalitis virus (CAEV) were evaluated by semiquantitative reverse transcriptase PCR for gamma interferon (IFN-gamma), interleukin-4 (IL-4), and IL-2 gene expression following in vitro stimulation with purified CAEV gp135 surface protein (SU). Studies examined three goats with chronic arthritis and four clinically asymptomatic goats at 5 years postinfection. SU-responsive IFN-gamma mRNA-positive cells and IL-4 mRNA-positive cells in PBMC from infected goats reflected differences in lymphokine balance associated with disease status. IFN-gamma mRNA-positive cells were dominant in PBMC from asymptomatic goats, whereas SU-responsive IL-4 mRNA-positive cells were dominant in PBMC from goats with arthritis. IL-2 gene expression was not responsive to SU stimulation of PBMC from either asymptomatic or arthritic goats. Lymphokine mRNA profiles in SU-stimulated PBMC were dependent on the presence of CD4+ T lymphocytes. The results indicate that asymptomatic goats have a dominant population of CAEV SU-reactive T-helper 1 (Th1)-like lymphocytes in PBMC whereas goats with clinical arthritis have a dominant population of SU-reactive Th2-like lymphocytes.
ISSN:10985514
0022538X
DOI:10.1128/jvi.71.8.6259-6263.1997