Heterogeneity of Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes in Enterococci
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| Název: | Heterogeneity of Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes in Enterococci |
|---|---|
| Autoři: | Eung Chil Choi, Hee Jeong Yun, Yun Jeong Choi, Yu Hong Min, Jin Hwan Kwak, Jae Hee Jeong, Mi Ja Shim, Kyungwon Lee |
| Přispěvatelé: | Yu-Hong Min, Jae-Hee Jeong, Eung-Chil Choi, Jin-Hwan Kwak, Mi-Ja Shim, Kyungwon Lee, Hee-Jeong Yun, Yun-Jeong Choi, Lee, Kyung Won |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 47:3415-3420 |
| Informace o vydavateli: | American Society for Microbiology, 2003. |
| Rok vydání: | 2003 |
| Témata: | 0301 basic medicine, Enterococcus faecium/drug effects, Lac Operon/genetics, Enterococcus faecium, Molecular Sequence Data, Drug Resistance, Microbial Sensitivity Tests, 03 medical and health sciences, Streptogramin B/pharmacology, beta-Galactosidase/biosynthesis, Drug Resistance, Bacterial, Enterococcus faecalis, Macrolides/pharmacology, Anti-Bacterial Agents/pharmacology, Lincosamides, Plasmids/genetics, 0303 health sciences, Enterococcus faecalis/growth & development, Base Sequence, Bacterial, Enterococcus faecium/growth & development, beta-Galactosidase, Enterococcus faecalis/drug effects, Anti-Bacterial Agents, 3. Good health, Phenotype, Lac Operon, Streptogramin B, Macrolides, Plasmids |
| Popis: | We determined the macrolide resistance phenotypes of 241 clinical isolates of erythromycin-resistant enterococci (MICs, ≥1 μg/ml), including 147 Enterococcus faecalis strains and 94 Enterococcus faecium strains, collected from a hospital in Seoul, Korea, between 1999 and 2000. By the erythromycin (40 μg)-josamycin (100 μg) double-disk test, 93 strains were assigned to the constitutive macrolide, lincosamide, and streptogramin B (MLS B ) resistance (cMLS B ) phenotype, and the remaining 148 strains were assigned to the inducible MLS B resistance (iMLS B ) phenotype. Of the strains with the iMLS B phenotype, 36 exhibited a reversibly inducible MLS B (riMLS B ) phenotype, i.e., blunting of the erythromycin zone of inhibition, which indicates that the 16-membered-ring macrolide josamycin is a more effective inducer than the 14-membered-ring macrolide erythromycin. Sequence analysis of the regulatory regions of the erm (B) genes from all of the strains exhibiting the riMLS B phenotype revealed not only erm (Bv) [where v represents variant; previously erm (AMR)] ( n = 13), as reported previously, but also three kinds of erm (B) variants, which were designated erm (Bv1) ( n = 17), erm (Bv2) ( n = 3), and erm (Bv3) ( n = 3), respectively. In lacZ reporter gene assays of these variants, the 16-membered-ring macrolide tylosin had stronger inducibility than erythromycin at ≥0.1 μg/ml. These findings highlight the versatility of erm (B) in induction specificity. |
| Druh dokumentu: | Article |
| Popis souboru: | 3415~3420 |
| Jazyk: | English |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.47.11.3415-3420.2003 |
| Přístupová URL adresa: | https://aac.asm.org/content/47/11/3415.full.pdf https://pubmed.ncbi.nlm.nih.gov/14576096 https://yonsei.pure.elsevier.com/en/publications/heterogeneity-of-macrolide-lincosamide-streptogramin-b-resistance https://dialnet.unirioja.es/servlet/articulo?codigo=769730 http://europepmc.org/articles/PMC253760 https://journals.asm.org/doi/pdf/10.1128/AAC.47.11.3415-3420.2003 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC253760/ https://aac.asm.org/content/47/11/3415.short |
| Rights: | ASM Journals Non-Commercial TDM CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....3c76dda93faf4c7c2e2ac3b4cab06fbb |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | We determined the macrolide resistance phenotypes of 241 clinical isolates of erythromycin-resistant enterococci (MICs, ≥1 μg/ml), including 147 Enterococcus faecalis strains and 94 Enterococcus faecium strains, collected from a hospital in Seoul, Korea, between 1999 and 2000. By the erythromycin (40 μg)-josamycin (100 μg) double-disk test, 93 strains were assigned to the constitutive macrolide, lincosamide, and streptogramin B (MLS B ) resistance (cMLS B ) phenotype, and the remaining 148 strains were assigned to the inducible MLS B resistance (iMLS B ) phenotype. Of the strains with the iMLS B phenotype, 36 exhibited a reversibly inducible MLS B (riMLS B ) phenotype, i.e., blunting of the erythromycin zone of inhibition, which indicates that the 16-membered-ring macrolide josamycin is a more effective inducer than the 14-membered-ring macrolide erythromycin. Sequence analysis of the regulatory regions of the erm (B) genes from all of the strains exhibiting the riMLS B phenotype revealed not only erm (Bv) [where v represents variant; previously erm (AMR)] ( n = 13), as reported previously, but also three kinds of erm (B) variants, which were designated erm (Bv1) ( n = 17), erm (Bv2) ( n = 3), and erm (Bv3) ( n = 3), respectively. In lacZ reporter gene assays of these variants, the 16-membered-ring macrolide tylosin had stronger inducibility than erythromycin at ≥0.1 μg/ml. These findings highlight the versatility of erm (B) in induction specificity. |
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| ISSN: | 10986596 00664804 |
| DOI: | 10.1128/aac.47.11.3415-3420.2003 |