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Zinc distribution within breast cancer tissue of different intrinsic subtypes

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Bibliographic Details
Title: Zinc distribution within breast cancer tissue of different intrinsic subtypes
Authors: Peter Rusch, Alfred V. Hirner, Oliver Schmitz, Rainer Kimmig, Oliver Hoffmann, Maxim Diel
Source: Arch Gynecol Obstet
Publisher Information: Springer Science and Business Media LLC, 2020.
Publication Year: 2020
Subject Terms: Adult, 2. Zero hunger, 0301 basic medicine, 0303 health sciences, Receptor, ErbB-2, Chemie, Medizin, Breast Neoplasms, Pilot Projects, Gynecologic Oncology, Middle Aged, Aged [MeSH], Receptor, ErbB-2/metabolism [MeSH], Breast cancer, Receptors, Progesterone/analysis [MeSH], Germany/epidemiology [MeSH], Feasibility Studies [MeSH], Receptor, ErbB-2/analysis [MeSH], Biomarker, Zinc, Zinc/metabolism [MeSH], Zinc/analysis [MeSH], Biomarkers, Tumor/analysis [MeSH], Steroid receptor, Breast Neoplasms/pathology [MeSH], Laser Therapy [MeSH], Female [MeSH], Human epidermal growth factor receptor 2, Receptors, Estrogen/metabolism [MeSH], Adult [MeSH], Receptors, Progesterone/metabolism [MeSH], Humans [MeSH], Breast Neoplasms/chemistry [MeSH], Middle Aged [MeSH], Zinc/chemistry [MeSH], Laser ablation inductively coupled mass spectrometry (LA-ICPMS), Biomarkers, Tumor/metabolism [MeSH], Pilot Projects [MeSH], Mass Spectrometry/methods [MeSH], Receptors, Estrogen/analysis [MeSH], Mass Spectrometry, 3. Good health, 03 medical and health sciences, Receptors, Estrogen, Germany, Biomarkers, Tumor, Feasibility Studies, Humans, Female, Laser Therapy, Receptors, Progesterone, Aged
Description: Purpose To show feasibility of laser ablation inductively coupled mass spectrometry (LA-ICPMS) for analysis of zinc content and concentration in breast cancer tissue and to correlate this with validated prognostic and predictive markers, i.e. histological grading and expression of steroid receptors (estrogen receptor, ER; progesterone receptor, PR) and human epidermal growth-factor receptor 2 (Her2). Methods 28 samples of human invasive ductal breast cancer tissue were subclassified into groups of four different intrinsic subtypes according to the expression of ER, PR and Her2 by immunohistological staining and then analyzed for zinc content and distribution by LA-ICPMS applying a calibration technique based on spiked polyacrylamide gels. A correlation of zinc concentration with histological grading and molecular subtypes was analyzed. Results Consistent with results of a pilot-study LA-ICPMS was feasible to show zinc accumulation in cancerous tissue, even more adjacent healthy stroma was with proportional increase of zinc. Zinc levels were most elevated in triple-positive (TPBC) and in triple-negative (TNB) breast cancers. Conclusion LA-ICPMS was feasible to confirm a connection between zinc and grade of malignancy; furthermore, focusing on a correlation of zinc and intrinsic breast cancer subtypes, LA-ICPMS depicted an upwards trend of zinc for “high-risk-cancers” with highest levels in Her2-positive and in triple-negative (TNBC) disease. The currently uncommon alliance of clinicians and analytical chemists in basic research is most promising to exploit the full potential of diagnostic accuracy in the efforts to solve the enigma of breast cancer initiation and course of disease.
Document Type: Article
Other literature type
Language: English
ISSN: 1432-0711
0932-0067
DOI: 10.1007/s00404-020-05789-8
Access URL: https://link.springer.com/content/pdf/10.1007/s00404-020-05789-8.pdf
https://pubmed.ncbi.nlm.nih.gov/32930875
https://pubmed.ncbi.nlm.nih.gov/32930875/
https://europepmc.org/article/MED/32930875
https://link.springer.com/content/pdf/10.1007/s00404-020-05789-8.pdf
https://link.springer.com/article/10.1007/s00404-020-05789-8
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854450
https://www.ncbi.nlm.nih.gov/pubmed/32930875
https://repository.publisso.de/resource/frl:6466708
https://www.ncbi.nlm.nih.gov/pubmed/32930875
https://doi.org/10.1007/s00404-020-05789-8
https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85090995657
Rights: CC BY
Accession Number: edsair.doi.dedup.....3abe9f4d33f61cfd42f6c2183070dc6a
Database: OpenAIRE
Description
Abstract:Purpose To show feasibility of laser ablation inductively coupled mass spectrometry (LA-ICPMS) for analysis of zinc content and concentration in breast cancer tissue and to correlate this with validated prognostic and predictive markers, i.e. histological grading and expression of steroid receptors (estrogen receptor, ER; progesterone receptor, PR) and human epidermal growth-factor receptor 2 (Her2). Methods 28 samples of human invasive ductal breast cancer tissue were subclassified into groups of four different intrinsic subtypes according to the expression of ER, PR and Her2 by immunohistological staining and then analyzed for zinc content and distribution by LA-ICPMS applying a calibration technique based on spiked polyacrylamide gels. A correlation of zinc concentration with histological grading and molecular subtypes was analyzed. Results Consistent with results of a pilot-study LA-ICPMS was feasible to show zinc accumulation in cancerous tissue, even more adjacent healthy stroma was with proportional increase of zinc. Zinc levels were most elevated in triple-positive (TPBC) and in triple-negative (TNB) breast cancers. Conclusion LA-ICPMS was feasible to confirm a connection between zinc and grade of malignancy; furthermore, focusing on a correlation of zinc and intrinsic breast cancer subtypes, LA-ICPMS depicted an upwards trend of zinc for “high-risk-cancers” with highest levels in Her2-positive and in triple-negative (TNBC) disease. The currently uncommon alliance of clinicians and analytical chemists in basic research is most promising to exploit the full potential of diagnostic accuracy in the efforts to solve the enigma of breast cancer initiation and course of disease.
ISSN:14320711
09320067
DOI:10.1007/s00404-020-05789-8