MicroRNA super-resolution imaging in blood for Alzheimer’s disease
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| Názov: | MicroRNA super-resolution imaging in blood for Alzheimer’s disease |
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| Autori: | Mirae Lee, Jiwon Woo, Sang Tae Kim, Minho Moon, Sang Yun Kim, Hanna Cho, Sujin Kim, Han-Kyeol Kim, Jeong-Yoon Park |
| Prispievatelia: | Mirae Lee, Jiwon Woo, Sang Tae Kim, Minho Moon, Sang Yun Kim, Hanna Cho, Sujin Kim, Han-Kyeol Kim, Jeong-Yoon Park, Kim, Han kyeol |
| Zdroj: | BMB Rep |
| Informácie o vydavateľovi: | Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2023. |
| Rok vydania: | 2023 |
| Predmety: | 0301 basic medicine, Alzheimer Disease* / diagnostic imaging, Hippocampus / diagnostic imaging, Article, Mice, 03 medical and health sciences, MicroRNAs* / genetics, Hippocampus / metabolism, Neurodegenerative Diseases* / metabolism, Leukocytes, Animals, Alzheimer Disease* / genetics, MicroRNAs* / metabolism, Mononuclear / metabolism |
| Popis: | We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer’s disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1976-670X |
| DOI: | 10.5483/bmbrep.2022-0151 |
| Rights: | CC BY NC CC BY NC ND URL: http://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (http://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Prístupové číslo: | edsair.doi.dedup.....3a5883bc6a187bf0d465d58a3ecccd81 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer’s disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases. |
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| ISSN: | 1976670X |
| DOI: | 10.5483/bmbrep.2022-0151 |