A combinatorial culture strategy to develop pseudomyxoma peritonei organoid models
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| Title: | A combinatorial culture strategy to develop pseudomyxoma peritonei organoid models |
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| Authors: | Luca Varinelli, Marzia Di Bella, Marcello Guaglio, Davide Battistessa, Federica Pisati, Tommaso Cavalleri, Massimo Milione, Jordi Martínez‐Quintanilla, Patrick T. Caswell, Dario Baratti, Shigeki Kusamura, Marcello Deraco, Manuela Gariboldi |
| Contributors: | Institut Català de la Salut, [Varinelli L, Di Bella M, Battistessa D] Molecular Epigenomics Unit, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale Tumori di Milano, Milan, Italy. [Guaglio M, Cavalleri T] Peritoneal Surface Malignancies Unit, Department of Surgery, Fondazione IRCCS Istituto Nazionale Tumori di Milano, Milan, Italy. [Pisati F] Cogentech Ltd. Benefit Corporation with a Sole Shareholder, Milan, Italy. [Martínez‐Quintanilla J] Translational Program, Stem Cells and Cancer Laboratory, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Source: | J Surg Oncol Scientia Scientia. Dipòsit d'Informació Digital del Departament de Salut instname |
| Publisher Information: | Wiley, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | Imatgeria tridimensional en medicina, ANATOMY::Tissues::Organoids, ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias abdominales::neoplasias peritoneales, ANATOMÍA::tejidos::organoides, ENFERMEDADES::neoplasias::neoplasias por tipo histológico::neoplasias glandulares y epiteliales::neoplasias quísticas, mucinosas y serosas::seudomixoma peritoneal, DISEASES::Neoplasms::Neoplasms by Site::Abdominal Neoplasms::Peritoneal Neoplasms, Mucines, DISEASES::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Glandular and Epithelial::Neoplasms, Cystic, Mucinous, and Serous::Pseudomyxoma Peritonei, Peritoneu - Càncer, Cultiu cel·lular, ANATOMY::Cells::Cells, Cultured::Tumor Cells, Cultured, ANATOMÍA::células::células cultivadas::células tumorales cultivadas, Research Article |
| Description: | Background and ObjectivesFew preclinical models of pseudomyxoma peritonei (PMP) have been developed, probably due to the tumor's low incidence and its peculiar characteristics of slow growth. Therefore, there is a need to develop more refined PMP models that better reflect its characteristics. The aim of the study is to develop a culture strategy to generate organoid models derived from PMP patient samples.MethodsWe followed a strategy based on combinatorial culture conditions that include the different factors essential for PMP growth and that mimic the microenvironment present in the patients.ResultsWe cultured PMP samples in the presence of the various factors produced by the niche environment of PMP. We obtained 12 PMP organoid models, each of which grows under specific culture conditions. PMP‐derived organoids show long‐term expansion capacity and reproduce the genetic landscape and histological phenotype of the tumor of origin.ConclusionThe organoids we developed faithfully reproduce the key features of PMP disease and will allow us to understand the biology of PMP. With them, we will be able to identify key regulatory networks that support PMP progression, providing a platform for multilevel preclinical testing, identify novel diagnostic biomarkers, and generate novel targets for patient treatments. |
| Document Type: | Article Other literature type |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1096-9098 0022-4790 |
| DOI: | 10.1002/jso.27850 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/39360464 https://hdl.handle.net/11351/12614 |
| Rights: | CC BY NC |
| Accession Number: | edsair.doi.dedup.....37b96416a021ff86befb076e73277c32 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Background and ObjectivesFew preclinical models of pseudomyxoma peritonei (PMP) have been developed, probably due to the tumor's low incidence and its peculiar characteristics of slow growth. Therefore, there is a need to develop more refined PMP models that better reflect its characteristics. The aim of the study is to develop a culture strategy to generate organoid models derived from PMP patient samples.MethodsWe followed a strategy based on combinatorial culture conditions that include the different factors essential for PMP growth and that mimic the microenvironment present in the patients.ResultsWe cultured PMP samples in the presence of the various factors produced by the niche environment of PMP. We obtained 12 PMP organoid models, each of which grows under specific culture conditions. PMP‐derived organoids show long‐term expansion capacity and reproduce the genetic landscape and histological phenotype of the tumor of origin.ConclusionThe organoids we developed faithfully reproduce the key features of PMP disease and will allow us to understand the biology of PMP. With them, we will be able to identify key regulatory networks that support PMP progression, providing a platform for multilevel preclinical testing, identify novel diagnostic biomarkers, and generate novel targets for patient treatments. |
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| ISSN: | 10969098 00224790 |
| DOI: | 10.1002/jso.27850 |