Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility
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| Název: | Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility |
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| Autoři: | Parreira, Gabriela Machado, Resende, Maria Daniela Aparecida, Garcia, Israel José Pereira, Sartori, Daniela Bueno, Umeoka, Eduardo Henrique de Lima, Godoy, Lívea Dornela, Garcia-Cairasco, Norberto, Barbosa, Leandro Augusto, Santos, Hérica de Lima, Tilelli, Cristiane Queixa |
| Zdroj: | Parreira, G M, Resende, M D A, Garcia, I J P, Sartori, D B, Umeoka, E H D L, Godoy, L D, Garcia-Cairasco, N, Barbosa, L A, Santos, H D L & Tilelli, C Q 2018, ' Oxidative stress and Na,K-ATPase activity differential regulation in brainstem and forebrain of Wistar Audiogenic rats may lead to increased seizure susceptibility ', Brain Research, vol. 1679, pp. 171-178 . https://doi.org/10.1016/j.brainres.2017.12.001 |
| Informace o vydavateli: | Elsevier BV, 2018. |
| Rok vydání: | 2018 |
| Témata: | 0301 basic medicine, Kindling, Neurologic/physiology, Sodium-Potassium-Exchanging ATPase/metabolism, Oxidative Stress/physiology, Seizures/etiology, 03 medical and health sciences, Neurons/enzymology, Prosencephalon, 0302 clinical medicine, Seizures, Kindling, Neurologic, Animals, Protein Isoforms, Rats, Wistar, Adenosine Triphosphatases, Neurons, 2. Zero hunger, Glutathione Peroxidase, Prosencephalon/enzymology, Glutathione Peroxidase/metabolism, Adenosine Triphosphatases/metabolism, Rats, Disease Models, Animal, Oxidative Stress, Acoustic Stimulation/adverse effects, Acoustic Stimulation, Brain Stem/enzymology, Lipid Peroxidation, Sodium-Potassium-Exchanging ATPase, Protein Isoforms/metabolism, Brain Stem |
| Popis: | The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling). In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples. We observed increased Na,K-ATPase activity in forebrain samples and increased oxidative stress markers (lipid peroxidation, glutathione peroxidase and superoxide dismutase) in brainstem samples of WAR. The Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and expression levels of α1 and α3 isoforms of Na,K-ATPase were unaltered. In view of previous data showing that the membrane potentials from naïve WAR's neurons are less negative than that from neurons from Wistar rats, we suggest that Na,K-ATPase increased activity might be involved in a compensatory mechanism necessary to maintain WAR's brains normal activity. Additionally, ongoing oxidative stress in the brainstem could bring Na,K-ATPase activity back to normal levels, which may explain why WAR's present increased susceptibility to seizures triggered by high-intensity sound stimulation. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2017.12.001 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/29225049 https://www.sciencedirect.com/science/article/pii/S0006899317305358 https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802274788340946 https://www.ncbi.nlm.nih.gov/pubmed/29225049 https://www.sciencedirect.com/science/article/pii/S0006899317305358#! |
| Rights: | Elsevier TDM |
| Přístupové číslo: | edsair.doi.dedup.....3621694a1bcb37626e6a73687a7357b5 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling). In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats. We also evaluated the expression levels of α1 and α3 isoforms of Na,K-ATPase in forebrain samples. We observed increased Na,K-ATPase activity in forebrain samples and increased oxidative stress markers (lipid peroxidation, glutathione peroxidase and superoxide dismutase) in brainstem samples of WAR. The Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and expression levels of α1 and α3 isoforms of Na,K-ATPase were unaltered. In view of previous data showing that the membrane potentials from naïve WAR's neurons are less negative than that from neurons from Wistar rats, we suggest that Na,K-ATPase increased activity might be involved in a compensatory mechanism necessary to maintain WAR's brains normal activity. Additionally, ongoing oxidative stress in the brainstem could bring Na,K-ATPase activity back to normal levels, which may explain why WAR's present increased susceptibility to seizures triggered by high-intensity sound stimulation. |
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| ISSN: | 00068993 |
| DOI: | 10.1016/j.brainres.2017.12.001 |