Lipid mediators involved in the oxidative stress and antioxidant defence of human lung cancer cells
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| Název: | Lipid mediators involved in the oxidative stress and antioxidant defence of human lung cancer cells |
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| Autoři: | Gęgotek, Agnieszka, Nikliński, Jacek, Žarković, Neven, Žarković, Kamelija, Waeg, Georg, Łuczaj, Wojciech, Charkiewicz, Radosław, Skrzydlewska, Elżbieta |
| Zdroj: | Redox Biol Redox Biology, Vol 9, Iss C, Pp 210-219 (2016) |
| Informace o vydavateli: | Elsevier BV, 2016. |
| Rok vydání: | 2016 |
| Témata: | Lung adenocarcinoma, Male, 0301 basic medicine, Catalase - metabolism, Medicine (General), Lung Neoplasms, Signal transduction, Regulacja ekspresji genu, Antioxidants, Antioxidant response elements, Endocannabinoids / metabolism, reactive aldehydes, Malondialdehyde, lung squamous cell carcinoma, Metabolizm lipidów, endocannabinoid system, Biology (General), Stres oksydacyjny, Gene expression regulation, 0303 health sciences, Regiony odpowiedzi przeciwoksydacyjnej, Lung neoplasms - metabolism, Dialdehyd malonowy - metabolizm, Lung neoplasms - genetics, Middle Aged, Catalase, Peroksydacja lipidów, Lung Neoplasms / metabolism, Markery biologiczne, 3. Good health, Glutathione Peroxidase / metabolism, Reakcje redoks, Linie komórkowe nowotworowe, Endokannabinoidy - metabolizm, NF-E2-Related Factor 2 / metabolism, Female, Malondialdehyde / metabolism, Katalaza - metabolizm, Oxidation-Reduction, Signal Transduction, Reactive aldehydes, Research Paper, tumor, Endocannabinoid system, QH301-705.5, NF-E2-Related Factor 2, Lipid peroxidation, Uszkodzenie DNA, Endocannabinoids - metabolism, Nrf2, NRF2, Nowotwory płuc - metabolizm, 03 medical and health sciences, R5-920, lung adenocarcinoma, Transdukcja sygnału, Cell Line, Tumor, Lung squamous cell carcinoma, Humans, Malondialdehyde - metabolism, Catalase / metabolism, Aged, Lung Neoplasms / genetics, Glutathione Peroxidase, Glutathione peroxidase - metabolism, Nowotwory płuc - genetyka, Lipid Metabolism, Antioxidants / metabolism, NF-E2-Related Factor 2 - metabolizm, Antioxidant Response Elements, Oxidation-reduction, Antioxidants - metabolism, Oxidative Stress, Lipid metabolism, NF-E2-related factor 2 - metabolism, Gene Expression Regulation, Oxidative stress, Antyoksydanty - metabolizm, Peroksydaza glutationowa - metabolizm, DNA damage, Lipid Peroxidation, Cell line, Biomarkers, DNA Damage, Endocannabinoids |
| Popis: | The oxidative modifications of bioactive macromolecules have important roles in carcinogenesis. Of particular interest are lipid peroxidation products, which are involved in the activation of Nrf2 and endocannabinoids that affect cancer progression.In lung cancer tissues (squamous cell lung carcinoma - SCC and adenocarcinoma - AC), the glutathione peroxidase and catalase activity and glutathione level, together with the expression of Nrf2 and its activators/inhibitors were estimated. The oxidative modifications of DNA (8-hydroxy-2'-deoxyguanosine and N7-methylguanine), endocannabinoids (anandamide and 2- arachidonylglyceriol), their receptors (CB1/2, TRV1, GPR55), phospholipid fatty acids (arachidonic, linoleic and docosahexaenoic), and reactive aldehydes (4-hydroxynonenal, 4-oxononenal and malondialdehyde) were determined.Tumour tissues showed lower antioxidant capacity than healthy tissues, which was accompanied by lower levels of fatty acids and higher levels of reactive aldehydes. Disturbances in antioxidant capacity and enhanced DNA oxidative modifications were observed in 88% of AC patients and 81% of SCC patients. The 4-hydroxynonenal-Histidine adducts were detected in the necrotic and stromal cells in all tumours. These findings were associated with the enhanced Nrf2 activity, especially in AC. The strong difference between the cancer subtypes was evident in the levels of endocannabinoids, with an increase in 89% of SCC and a decrease in 85% of AC patients being observed. Additionally, the increase in the expression of CB1/2 receptors was observed only in 82% of AC, while the expression of VR1 and GPR55 was enhanced in 79% of SCC and 82% of AC patients.This study shows significant differences in the redox status, Nrf2 pathway and endocannabinoid system between SCC and AC tissues. Understanding the relation between the various lipid mediators and antioxidants in different lung cancer subtypes may be beginning for further research on the effective anticancer therapy. |
| Druh dokumentu: | Article Other literature type |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 2213-2317 |
| DOI: | 10.1016/j.redox.2016.08.010 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/27567474 https://doaj.org/article/52b4392d95c64cd1ac95dba05688ae01 https://core.ac.uk/display/91049607 https://www.bib.irb.hr/941527 https://www.ncbi.nlm.nih.gov/pubmed/27567474 https://pubmed.ncbi.nlm.nih.gov/27567474/ https://www.sciencedirect.com/science/article/pii/S2213231716301252 http://www.ncbi.nlm.nih.gov/pubmed/27567474 https://urn.nsk.hr/urn:nbn:hr:105:938999 https://doi.org/10.1016/j.redox.2016.08.010 |
| Rights: | CC BY NC ND URL: http://rightsstatements.org/vocab/InC/1.0/ |
| Přístupové číslo: | edsair.doi.dedup.....31d1fc815cd69c33742fbd23d28cca7a |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The oxidative modifications of bioactive macromolecules have important roles in carcinogenesis. Of particular interest are lipid peroxidation products, which are involved in the activation of Nrf2 and endocannabinoids that affect cancer progression.In lung cancer tissues (squamous cell lung carcinoma - SCC and adenocarcinoma - AC), the glutathione peroxidase and catalase activity and glutathione level, together with the expression of Nrf2 and its activators/inhibitors were estimated. The oxidative modifications of DNA (8-hydroxy-2'-deoxyguanosine and N7-methylguanine), endocannabinoids (anandamide and 2- arachidonylglyceriol), their receptors (CB1/2, TRV1, GPR55), phospholipid fatty acids (arachidonic, linoleic and docosahexaenoic), and reactive aldehydes (4-hydroxynonenal, 4-oxononenal and malondialdehyde) were determined.Tumour tissues showed lower antioxidant capacity than healthy tissues, which was accompanied by lower levels of fatty acids and higher levels of reactive aldehydes. Disturbances in antioxidant capacity and enhanced DNA oxidative modifications were observed in 88% of AC patients and 81% of SCC patients. The 4-hydroxynonenal-Histidine adducts were detected in the necrotic and stromal cells in all tumours. These findings were associated with the enhanced Nrf2 activity, especially in AC. The strong difference between the cancer subtypes was evident in the levels of endocannabinoids, with an increase in 89% of SCC and a decrease in 85% of AC patients being observed. Additionally, the increase in the expression of CB1/2 receptors was observed only in 82% of AC, while the expression of VR1 and GPR55 was enhanced in 79% of SCC and 82% of AC patients.This study shows significant differences in the redox status, Nrf2 pathway and endocannabinoid system between SCC and AC tissues. Understanding the relation between the various lipid mediators and antioxidants in different lung cancer subtypes may be beginning for further research on the effective anticancer therapy. |
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| ISSN: | 22132317 |
| DOI: | 10.1016/j.redox.2016.08.010 |