The cytoskeleton adaptor protein Sorbs1 controls the development of lymphatic and venous vessels in zebrafish
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| Názov: | The cytoskeleton adaptor protein Sorbs1 controls the development of lymphatic and venous vessels in zebrafish |
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| Autori: | Veloso, Alexandra, Bleuart, Anouk, Conrard, Louise, Orban, Tanguy, Bruyr, Jonathan, Cabochette, Pauline, Germano, Raoul F.V., Schevenels, Giel, Bernard, Alice, Zindy, Egor, Demeyer, Sofie, Vanhollebeke, Benoit, Dequiedt, Franck, Martin, Maud |
| Prispievatelia: | F.R.S.-FNRS - Fonds de la Recherche Scientifique |
| Zdroj: | BMC Biol BMC Biology, Vol 22, Iss 1, Pp 1-23 (2024) BMC Biology |
| Informácie o vydavateľovi: | Springer Science and Business Media LLC, 2024. |
| Rok vydania: | 2024 |
| Predmety: | Life Sciences & Biomedicine - Other Topics, 0301 basic medicine, Zebrafish Proteins/metabolism, Physiology, Plant Science, Structural Biology, Zebrafish Proteins -- genetics -- metabolism, Rho GTPases, Sorbs1, Biology (General), Lymphangiogenesis, Migration, Zebrafish, Cytoskeleton, 0303 health sciences, ALPHA-ACTININ, Lymphatic Vessels -- metabolism, Adaptor Proteins, Sciences bio-médicales et agricoles, Cytoskeleton -- metabolism, Life sciences, Signal Transducing -- metabolism, PROX1 EXPRESSION, Lymphangiogenesis -- physiology, Sciences du vivant, Adhesion, General Agricultural and Biological Sciences, Life Sciences & Biomedicine, Biotechnology, Research Article, BMP signaling, QH301-705.5, FOCAL ADHESION KINASE, Biochimie, biophysique & biologie moléculaire, EMBRYONIC LYMPHANGIOGENESIS, VINEXIN, General Biochemistry, Genetics and Molecular Biology, Zebrafish/genetics, 03 medical and health sciences, Zebrafish/metabolism, Zebrafish Proteins/genetics, Vegfc, Animals, GROWTH-FACTOR-C, Biology, INTERACTING PROTEIN, Ecology, Evolution, Behavior and Systematics, Lymphatic Vessels, Adaptor Proteins, Signal Transducing, Lymphatic Vessels/metabolism, Biochemistry, Genetics and Molecular Biology (all), Science & Technology, 31 Biological sciences, Zebrafish -- genetics -- metabolism, Cell Biology, STRESS FIBERS, 06 Biological Sciences, Zebrafish Proteins, Lymphangiogenesis/physiology, Adaptor Proteins, Signal Transducing/metabolism, Agricultural and Biological Sciences (all), Cytoskeleton/metabolism, Angiogenesis, CARDIAC LYMPHATICS, VASCULAR DEVELOPMENT, Biochemistry, biophysics & molecular biology, Developmental Biology |
| Popis: | Background Lymphangiogenesis, the formation of lymphatic vessels, is tightly linked to the development of the venous vasculature, both at the cellular and molecular levels. Here, we identify a novel role for Sorbs1, the founding member of the SoHo family of cytoskeleton adaptor proteins, in vascular and lymphatic development in the zebrafish. Results We show that Sorbs1 is required for secondary sprouting and emergence of several vascular structures specifically derived from the axial vein. Most notably, formation of the precursor parachordal lymphatic structures is affected in sorbs1 mutant embryos, severely impacting the establishment of the trunk lymphatic vessel network. Interestingly, we show that Sorbs1 interacts with the BMP pathway and could function outside of Vegfc signaling. Mechanistically, Sorbs1 controls FAK/Src signaling and subsequently impacts on the cytoskeleton processes regulated by Rac1 and RhoA GTPases. Inactivation of Sorbs1 altered cell-extracellular matrix (ECM) contacts rearrangement and cytoskeleton dynamics, leading to specific defects in endothelial cell migratory and adhesive properties. Conclusions Overall, using in vitro and in vivo assays, we identify Sorbs1 as an important regulator of venous and lymphatic angiogenesis independently of the Vegfc signaling axis. These results provide a better understanding of the complexity found within context-specific vascular and lymphatic development. |
| Druh dokumentu: | Article Other literature type |
| Popis súboru: | 1 full-text file(s): application/pdf |
| Jazyk: | English |
| ISSN: | 1741-7007 |
| DOI: | 10.1186/s12915-024-01850-z |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/38414014 https://doaj.org/article/c363338c59c147acad9ab5d4c4848971 https://lirias.kuleuven.be/handle/20.500.12942/738865 https://doi.org/10.1186/s12915-024-01850-z |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (http://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| Prístupové číslo: | edsair.doi.dedup.....2f1bc13d40925e77da4793b0e2c47882 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Background Lymphangiogenesis, the formation of lymphatic vessels, is tightly linked to the development of the venous vasculature, both at the cellular and molecular levels. Here, we identify a novel role for Sorbs1, the founding member of the SoHo family of cytoskeleton adaptor proteins, in vascular and lymphatic development in the zebrafish. Results We show that Sorbs1 is required for secondary sprouting and emergence of several vascular structures specifically derived from the axial vein. Most notably, formation of the precursor parachordal lymphatic structures is affected in sorbs1 mutant embryos, severely impacting the establishment of the trunk lymphatic vessel network. Interestingly, we show that Sorbs1 interacts with the BMP pathway and could function outside of Vegfc signaling. Mechanistically, Sorbs1 controls FAK/Src signaling and subsequently impacts on the cytoskeleton processes regulated by Rac1 and RhoA GTPases. Inactivation of Sorbs1 altered cell-extracellular matrix (ECM) contacts rearrangement and cytoskeleton dynamics, leading to specific defects in endothelial cell migratory and adhesive properties. Conclusions Overall, using in vitro and in vivo assays, we identify Sorbs1 as an important regulator of venous and lymphatic angiogenesis independently of the Vegfc signaling axis. These results provide a better understanding of the complexity found within context-specific vascular and lymphatic development. |
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| ISSN: | 17417007 |
| DOI: | 10.1186/s12915-024-01850-z |