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Geographic diversity in Helicobacter pylori oipA genotype between Korean and United States isolates

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Název: Geographic diversity in Helicobacter pylori oipA genotype between Korean and United States isolates
Autoři: Jeong Heon Cha, Ji-Hye Kim, Aeryun Kim, Jing Lai, D. Scott Merrell, Hanfu Su
Přispěvatelé: Aeryun Kim, Jing Lai, D Scott Merrell, Ji-Hye Kim, Hanfu Su, Jeong-Heon Cha, Cha, Jung Heon
Zdroj: Journal of Microbiology. 59:1125-1132
Informace o vydavateli: The Microbiological Society of Korea, 2021.
Rok vydání: 2021
Témata: Adult, Male, 0301 basic medicine, Genotype, Phase Variation, Gene Dosage, Helicobacter Infections / epidemiology, outer membrane protein, Helicobacter Infections, Cytosine, 03 medical and health sciences, Helicobacter pylori / genetics, Consensus Sequence, Republic of Korea, 80 and over, geographic diversity, Humans, Dinucleotide Repeats, Republic of Korea / epidemiology, Aged, Aged, 80 and over, Molecular Epidemiology, 0303 health sciences, United States / epidemiology, Helicobacter pylori / isolation & purification, Helicobacter pylori, Virulence Factors / genetics, Bacterial Outer Membrane Proteins / genetics, Middle Aged, Helicobacter Infections / microbiology, United States, oipA, Female, Bacterial Outer Membrane Proteins, Thymidine
Popis: Helicobacter pylori outer membrane inflammatory protein A (OipA) was originally named for its role in inducing inflammation in the host, as evidenced by high mucosal IL-8 levels. Expression of OipA is regulated by phase variation of a CT dinucleotide-repeat located in the 5' region of the gene. However, little is known about OipA geographic diversity across isolates. To address this gap, we conducted a large-scale molecular epidemiologic analysis using H. pylori clinical isolates obtained from two geographically distinct populations: Korea and the United States (US). Most Korean isolates (98.7%) possessed two copies of oipA located at two specific loci (A and B) while all US isolates contained only one copy of oipA at locus A. Furthermore, most Korean oipA (94.8%) possessed three or less CT repeats while most US oipA (96.6%) contained five or more CT repeats. Among the two copies, all Korean H. pylori possessed at least one oipA 'on' phase variant while the single copy of oipA in US isolates showed 56.2% 'on' and 43.8% 'off.' Thus, host differences seem to have driven geographic diversification of H. pylori across these populations such that OipA expression in US isolates is still regulated by phase variation with 5 or more CT repeats, while Korean isolates always express OipA; duplication of the oipA combined with a reduction of CT repeats to three or less ensures continued expression. En masse, these findings suggest that diversity in the oipA gene copy number, CT repeats, and phase variation among H. pylori from different populations may confer a benefit in adaptation to particular host populations.
Druh dokumentu: Article
Jazyk: English
ISSN: 1976-3794
DOI: 10.1007/s12275-021-1450-8
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/34718962
https://link.springer.com/article/10.1007/s12275-021-1450-8
https://pubmed.ncbi.nlm.nih.gov/34718962/
Rights: Springer TDM
CC BY NC ND
Přístupové číslo: edsair.doi.dedup.....2dfc6f55e1db34e6b9dc5724500fa846
Databáze: OpenAIRE
Popis
Abstrakt:Helicobacter pylori outer membrane inflammatory protein A (OipA) was originally named for its role in inducing inflammation in the host, as evidenced by high mucosal IL-8 levels. Expression of OipA is regulated by phase variation of a CT dinucleotide-repeat located in the 5' region of the gene. However, little is known about OipA geographic diversity across isolates. To address this gap, we conducted a large-scale molecular epidemiologic analysis using H. pylori clinical isolates obtained from two geographically distinct populations: Korea and the United States (US). Most Korean isolates (98.7%) possessed two copies of oipA located at two specific loci (A and B) while all US isolates contained only one copy of oipA at locus A. Furthermore, most Korean oipA (94.8%) possessed three or less CT repeats while most US oipA (96.6%) contained five or more CT repeats. Among the two copies, all Korean H. pylori possessed at least one oipA 'on' phase variant while the single copy of oipA in US isolates showed 56.2% 'on' and 43.8% 'off.' Thus, host differences seem to have driven geographic diversification of H. pylori across these populations such that OipA expression in US isolates is still regulated by phase variation with 5 or more CT repeats, while Korean isolates always express OipA; duplication of the oipA combined with a reduction of CT repeats to three or less ensures continued expression. En masse, these findings suggest that diversity in the oipA gene copy number, CT repeats, and phase variation among H. pylori from different populations may confer a benefit in adaptation to particular host populations.
ISSN:19763794
DOI:10.1007/s12275-021-1450-8