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Melatonin reduces infarction volume in a photothrombotic stroke model in the wild‐type but not cyclooxygenase‐1‐gene knockout mice

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Název: Melatonin reduces infarction volume in a photothrombotic stroke model in the wild‐type but not cyclooxygenase‐1‐gene knockout mice
Autoři: Liang, YZ, Cheung, RTF, Huang, L, Liu, S, Li, G
Zdroj: Journal of Pineal Research. 41:150-156
Informace o vydavateli: Wiley, 2006.
Rok vydání: 2006
Témata: Thrombosis - metabolism, 0301 basic medicine, Knockout, Brain Edema, Brain - blood supply - drug effects - pathology, Mice, 03 medical and health sciences, Reperfusion Injury - physiopathology, Laser-Doppler Flowmetry, Cerebrovascular Circulation - drug effects, Animals, Melatonin - administration & dosage - therapeutic use, Neuroprotective Agents - therapeutic use, Melatonin, Mice, Knockout, Rose Bengal, 0303 health sciences, Cerebral Infarction - drug therapy - pathology - physiopathology, Animal, Brain, Thrombosis, Cerebral Infarction, Cyclooxygenase 1 - genetics, Brain Edema - pathology, Disease Models, Animal, Neuroprotective Agents, Motor Skills, Cerebrovascular Circulation, Reperfusion Injury, Disease Models, Cyclooxygenase 1
Popis: Cyclooxygenase (COX)‐2 plays a harmful role in cerebral ischemic/reperfusion injury, but the role of COX‐1 is uncertain. In the present study, cerebral infarct was induced by photothrombosis. Intraperitoneal injections of melatonin at 15 g/kg or its vehicle were made at 0.5 hr before stroke and 24 and 48 hr after stroke. Cerebral blood flow (CBF) in the penumbra was monitored during stroke using a laser Doppler flowmeter. Sensorimotor behavior was evaluated using the turning in an alley and falling from a pole tests at 1 hr before stroke and 24 and 48 hr after stroke. Infarct volume was determined from the T2‐weighted magnetic resonance images at 72 hr after stroke. During the first 15 min of stroke, CBF decreased in the penumbra in both homozygous COX‐1‐gene knockout and wild‐type mice. Melatonin treatment improved the penumbral CBF in the wild‐type mice. Mild poststroke impairment in sensorimotor behavior was detected by the turning in an alley test in which the COX‐1‐gene knockout mice performed better. Melatonin treatment did not affect the poststroke sensorimotor behavior. The relative infarct volume at 72 hr after stroke was 8.1% and 8.4% in the COX‐1‐gene knockout and wild‐type mice, respectively. Melatonin treatment reduced the relative infarct volume to 6.3% in the latter but not in the former (8.2%). Thus, COX‐1‐gene knockout does not affect the brain's susceptibility to photothrombotic stroke. Melatonin treatment reduces infarct size in the wild‐type mice following photothrombotic stroke partly via maintenance of penumbral CBF in which the COX‐1‐gene may play a role.
Druh dokumentu: Article
Jazyk: English
ISSN: 1600-079X
0742-3098
DOI: 10.1111/j.1600-079x.2006.00349.x
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/16879321
https://www.ncbi.nlm.nih.gov/pubmed/16879321
http://hub.hku.hk/handle/10722/78349
https://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2006.00349.x
https://pubmed.ncbi.nlm.nih.gov/16879321/
http://hdl.handle.net/10722/78349
Rights: Wiley Online Library User Agreement
Přístupové číslo: edsair.doi.dedup.....2c7d5a42333e0f9417845243599431ad
Databáze: OpenAIRE
Popis
Abstrakt:Cyclooxygenase (COX)‐2 plays a harmful role in cerebral ischemic/reperfusion injury, but the role of COX‐1 is uncertain. In the present study, cerebral infarct was induced by photothrombosis. Intraperitoneal injections of melatonin at 15 g/kg or its vehicle were made at 0.5 hr before stroke and 24 and 48 hr after stroke. Cerebral blood flow (CBF) in the penumbra was monitored during stroke using a laser Doppler flowmeter. Sensorimotor behavior was evaluated using the turning in an alley and falling from a pole tests at 1 hr before stroke and 24 and 48 hr after stroke. Infarct volume was determined from the T2‐weighted magnetic resonance images at 72 hr after stroke. During the first 15 min of stroke, CBF decreased in the penumbra in both homozygous COX‐1‐gene knockout and wild‐type mice. Melatonin treatment improved the penumbral CBF in the wild‐type mice. Mild poststroke impairment in sensorimotor behavior was detected by the turning in an alley test in which the COX‐1‐gene knockout mice performed better. Melatonin treatment did not affect the poststroke sensorimotor behavior. The relative infarct volume at 72 hr after stroke was 8.1% and 8.4% in the COX‐1‐gene knockout and wild‐type mice, respectively. Melatonin treatment reduced the relative infarct volume to 6.3% in the latter but not in the former (8.2%). Thus, COX‐1‐gene knockout does not affect the brain's susceptibility to photothrombotic stroke. Melatonin treatment reduces infarct size in the wild‐type mice following photothrombotic stroke partly via maintenance of penumbral CBF in which the COX‐1‐gene may play a role.
ISSN:1600079X
07423098
DOI:10.1111/j.1600-079x.2006.00349.x