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Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8α+ dendritic cells

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Titel: Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8α+ dendritic cells
Autoren: Poulin, Lionel, Salio, Mariolina, Griessinger, Emmanuel, Anjos-Afonso, Fernando, Ruscas-Craciun, Ligia Ioana, Chen, Ji-Li, Keller, Anna M, Joffre, Olivier, Zelenay, Santiago, Nye, Emma, Le Moine, Alain, Faure, Florence, Donckier De Donceel, Vincent, Sancho, David, Cerundolo, Vincenzo, Bonnet, Dominique, Reis e Sousa, Caetano
Quelle: J Exp Med
The Journal of experimental medicine, 207 (6
Poulin, L F, Salio, M, Griessinger, E, Anjos-Afonso, F, Craciun, L, Chen, J-L, Keller, A M, Joffre, O, Zelenay, S, Nye, E, Le Moine, A, Faure, F, Donckier, V, Sancho, D, Cerundolo, V, Bonnet, D & Reis e Sousa, C 2010, 'Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.', The Journal of experimental medicine, vol. 207, no. 6. https://doi.org/10.1084/jem.20092618
Verlagsinformationen: Rockefeller University Press, 2010.
Publikationsjahr: 2010
Schlagwörter: Poly I-C -- pharmacology, Thrombomodulin, Interleukin-12 -- biosynthesis, Spleen -- cytology -- drug effects -- immunology, CD8-Positive T-Lymphocytes, Dendritic Cells -- cytology -- drug effects -- immunology, Toll-Like Receptors -- agonists, Article, Immunity, Innate -- drug effects, Mice, 03 medical and health sciences, Cross-Priming, 0302 clinical medicine, Innate -- drug effects, Lectins, Receptors, Mitogen -- metabolism, Animals, Humans, Lectins, C-Type, Antigens, CD8-Positive T-Lymphocytes -- cytology -- drug effects -- immunology, Surface -- metabolism, Endocytosis -- drug effects -- immunology, Lectins, C-Type -- metabolism, Gene Expression Profiling, Hematopoietic Stem Cells -- cytology -- drug effects, Toll-Like Receptors, Immunity, Dendritic Cells, Sciences bio-médicales et agricoles, Hematopoietic Stem Cells, Interleukin-12, Endocytosis, Immunity, Innate, C-Type -- metabolism, 3. Good health, Antigens, Surface -- metabolism, Gene Expression Regulation -- drug effects, Phenotype, Poly I-C, Gene Expression Regulation, Receptors, Mitogen, Antigens, Surface, Receptors, Mitogen -- metabolism, Cross-Priming -- drug effects, Spleen
Beschreibung: In mouse, a subset of dendritic cells (DCs) known as CD8α+ DCs has emerged as an important player in the regulation of T cell responses and a promising target in vaccination strategies. However, translation into clinical protocols has been hampered by the failure to identify CD8α+ DCs in humans. Here, we characterize a population of human DCs that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8α+ DCs in phenotype and function. We describe the presence of such cells in the spleens of humans and humanized mice and report on a protocol to generate them in vitro. Like mouse CD8α+ DCs, human DNGR-1+ BDCA3hi DCs express Necl2, CD207, BATF3, IRF8, and TLR3, but not CD11b, IRF4, TLR7, or (unlike CD8α+ DCs) TLR9. DNGR-1+ BDCA3hi DCs respond to poly I:C and agonists of TLR8, but not of TLR7, and produce interleukin (IL)-12 when given innate and T cell–derived signals. Notably, DNGR-1+ BDCA3+ DCs from in vitro cultures efficiently internalize material from dead cells and can cross-present exogenous antigens to CD8+ T cells upon treatment with poly I:C. The characterization of human DNGR-1+ BDCA3hi DCs and the ability to grow them in vitro opens the door for exploiting this subset in immunotherapy.
Publikationsart: Article
Other literature type
Dateibeschreibung: 1 full-text file(s): application/pdf
Sprache: English
ISSN: 1540-9538
0022-1007
DOI: 10.1084/jem.20092618
Zugangs-URL: http://jem.rupress.org/content/207/6/1261.full.pdf
https://pubmed.ncbi.nlm.nih.gov/20479117
https://research.manchester.ac.uk/en/publications/e2686a1d-a4a1-4ca7-8cbf-7aae87f574ac
https://doi.org/10.1084/jem.20092618
https://www.research.manchester.ac.uk/portal/en/publications/characterization-of-human-dngr1-bdca3-leukocytes-as-putative-equivalents-of-mouse-cd8alpha-dendritic-cells(e2686a1d-a4a1-4ca7-8cbf-7aae87f574ac)/export.html
https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/128893/Details
http://europepmc.org/abstract/MED/20479117
https://europepmc.org/articles/PMC2882845
https://rupress.org/jem/article-pdf/207/6/1261/1203935/jem_20092618.pdf
https://ora.ox.ac.uk/objects/uuid:a53ef689-d20a-4797-bdd0-673de5a538f1
https://ora.ox.ac.uk/objects/uuid:a53ef689-d20a-4797-bdd0-673de5a538f1
https://doi.org/10.1084/jem.20092618
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/128893
Rights: CC BY NC SA
Dokumentencode: edsair.doi.dedup.....2c2ec683c70feb7029c013df02866176
Datenbank: OpenAIRE
Beschreibung
Abstract:In mouse, a subset of dendritic cells (DCs) known as CD8α+ DCs has emerged as an important player in the regulation of T cell responses and a promising target in vaccination strategies. However, translation into clinical protocols has been hampered by the failure to identify CD8α+ DCs in humans. Here, we characterize a population of human DCs that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8α+ DCs in phenotype and function. We describe the presence of such cells in the spleens of humans and humanized mice and report on a protocol to generate them in vitro. Like mouse CD8α+ DCs, human DNGR-1+ BDCA3hi DCs express Necl2, CD207, BATF3, IRF8, and TLR3, but not CD11b, IRF4, TLR7, or (unlike CD8α+ DCs) TLR9. DNGR-1+ BDCA3hi DCs respond to poly I:C and agonists of TLR8, but not of TLR7, and produce interleukin (IL)-12 when given innate and T cell–derived signals. Notably, DNGR-1+ BDCA3+ DCs from in vitro cultures efficiently internalize material from dead cells and can cross-present exogenous antigens to CD8+ T cells upon treatment with poly I:C. The characterization of human DNGR-1+ BDCA3hi DCs and the ability to grow them in vitro opens the door for exploiting this subset in immunotherapy.
ISSN:15409538
00221007
DOI:10.1084/jem.20092618