Zobraziť v EDS

In vitro evaluation of novel (nanoparticle) oral delivery systems allow selection of gut immunomodulatory formulations

Uložené v:
Podrobná bibliografia
Názov: In vitro evaluation of novel (nanoparticle) oral delivery systems allow selection of gut immunomodulatory formulations
Autori: Ahmed Attaya, Kimberly Veenstra, Michael D. Welsh, Mukhtar Ahmed, Nessim Torabi-Pour, Suzanne Saffie-Siebert, Sohye Yoon, Christopher J. Secombes
Zdroj: Fish & Shellfish Immunology. 113:125-138
Informácie o vydavateľovi: Elsevier BV, 2021.
Rok vydania: 2021
Predmety: Oral, 0301 basic medicine, Immunologic/administration & dosage, Administration, Oral, Macrophages/immunology, Aeromonas salmonicida, Adaptive Immunity, Monocytes, Cell Line, Aeromonas salmonicida/immunology, Fish Diseases, 03 medical and health sciences, Drug Delivery Systems, Adjuvants, Immunologic, Fish Diseases/immunology, Oncorhynchus mykiss/immunology, Bacterial/immunology, Innate, Animals, Adjuvants, Antigens, Gram-Negative Bacterial Infections/immunology, Antigens, Bacterial, 0303 health sciences, Macrophages, Vaccination, Immunity, Monocytes/immunology, Vaccination/instrumentation, Drug Delivery Systems/instrumentation, Nanoparticles/administration & dosage, Immunity, Innate, 3. Good health, Oncorhynchus mykiss, Administration, Bacterial Vaccines, Bacterial Vaccines/administration & dosage, Nanoparticles, Gram-Negative Bacterial Infections
Popis: Oral delivery is the most convenient way to vaccinate cultured fish, however it is still problematic, primarily due to a lack of a commercially valid vaccine vehicle to protect the antigen against gastric degradation and ensure its uptake from the intestine. With the goal of advancing the potential to vaccinate orally, this study evaluates a novel silicon nanoparticle-based vehicle (VacSaf carrier). Aeromonas salmonicida antigens were formulated with the VacSaf carrier using different preparation methods to generate dry powder and liquid formulations. Twelve formulations were first subjected to an in vitro evaluation where the A. salmonicida bacterin conjugated to VacSaf carriers were found superior at inducing pro-inflammatory cytokine expression in primary leucocyte cultures and the macrophage/monocyte cell line RTS-11 compared with A. salmonicida bacterin alone. This was especially apparent after exposure to acid conditions to mimic stomach processing. One formulation (FD1) was taken forward to oral delivery using two doses and two administration schedules (5 days vs 10 days, the latter 5 days on, 5 days off, 5 days on), and the transcript changes of immune genes in the intestine (pyloric caeca, midgut and hindgut) and spleen were evaluated by qPCR and serum IgM was measured by ELISA. The VacSaf carrier alone was shown to be safe for use in vivo, in that no side-effects were seen, but it did induce expression of some cytokines, and may have value as an oral adjuvant candidate. The FD1 bacterin formulation was effective at inducing a range of cytokines associated with innate and adaptive immunity, mainly in the pyloric caeca, compared to A. salmonicida bacterin alone (which had almost no effect), and confirms the immune competence of this gut region following appropriate oral vaccination. These results reveal that in vitro screening of formulations for oral delivery has value and can be used to assess the most promising formulations to test further.
Druh dokumentu: Article
Jazyk: English
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2021.03.007
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/33746060
https://pubmed.ncbi.nlm.nih.gov/33746060/
https://europepmc.org/article/MED/33746060
https://www.sciencedirect.com/science/article/pii/S1050464821000668
http://www.ncbi.nlm.nih.gov/pubmed/33746060
Rights: Elsevier TDM
Prístupové číslo: edsair.doi.dedup.....29b4686102f08560b1317fb1f65b0bee
Databáza: OpenAIRE
Popis
Abstrakt:Oral delivery is the most convenient way to vaccinate cultured fish, however it is still problematic, primarily due to a lack of a commercially valid vaccine vehicle to protect the antigen against gastric degradation and ensure its uptake from the intestine. With the goal of advancing the potential to vaccinate orally, this study evaluates a novel silicon nanoparticle-based vehicle (VacSaf carrier). Aeromonas salmonicida antigens were formulated with the VacSaf carrier using different preparation methods to generate dry powder and liquid formulations. Twelve formulations were first subjected to an in vitro evaluation where the A. salmonicida bacterin conjugated to VacSaf carriers were found superior at inducing pro-inflammatory cytokine expression in primary leucocyte cultures and the macrophage/monocyte cell line RTS-11 compared with A. salmonicida bacterin alone. This was especially apparent after exposure to acid conditions to mimic stomach processing. One formulation (FD1) was taken forward to oral delivery using two doses and two administration schedules (5 days vs 10 days, the latter 5 days on, 5 days off, 5 days on), and the transcript changes of immune genes in the intestine (pyloric caeca, midgut and hindgut) and spleen were evaluated by qPCR and serum IgM was measured by ELISA. The VacSaf carrier alone was shown to be safe for use in vivo, in that no side-effects were seen, but it did induce expression of some cytokines, and may have value as an oral adjuvant candidate. The FD1 bacterin formulation was effective at inducing a range of cytokines associated with innate and adaptive immunity, mainly in the pyloric caeca, compared to A. salmonicida bacterin alone (which had almost no effect), and confirms the immune competence of this gut region following appropriate oral vaccination. These results reveal that in vitro screening of formulations for oral delivery has value and can be used to assess the most promising formulations to test further.
ISSN:10504648
DOI:10.1016/j.fsi.2021.03.007