Zobraziť v EDS

DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories

Uložené v:
Podrobná bibliografia
Názov: DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories
Autori: Nieto, Esteban E., Jurburg, Stephanie D., Steinbach, Nicole, Festa, Sabrina, Morelli, Irma S., Coppotelli, Bibiana M., Chatzinotas, Antonis
Zdroj: Microbiome
Microbiome, Vol 12, Iss 1, Pp 1-12 (2024)
Microbiome, 12(1):146
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2024.
Rok vydania: 2024
Predmety: 0301 basic medicine, Food Chain, bacterial consortia, Microbial ecology, Soil, 03 medical and health sciences, RNA, Ribosomal, 16S, RNA, Ribosomal, 18S, Soil Pollutants, Biomass, Polycyclic Aromatic Hydrocarbons, bioaugmentation, Soil Microbiology, 2. Zero hunger, Carbon Isotopes, 0303 health sciences, Bacteria, Research, QR100-130, 15. Life on land, 6. Clean water, Biodegradation, Environmental, 13. Climate action, Isotope Labeling, predation, Bacteria/metabolism [MeSH], Biomass [MeSH], Engineering Microbiomes for Green Technologies, Soil Pollutants/metabolism [MeSH], RNA, Ribosomal, 18S/genetics [MeSH], RNA, Ribosomal, 16S/genetics [MeSH], Bacteria/genetics [MeSH], Carbon Isotopes/metabolism [MeSH], Soil/chemistry [MeSH], Biodegradation, Environmental [MeSH], Soil Microbiology [MeSH], Isotope Labeling [MeSH], Bacteria/classification [MeSH], Food Chain [MeSH], Polycyclic Aromatic Hydrocarbons/metabolism [MeSH], SIP-DNA
Popis: Background Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable. Predation is a key top-down control mechanism affecting inoculum establishment, however, its effects on this process have received little attention. This study focused on the impact of trophic interactions on bioaugmentation success in two soils with different pollution exposure histories. We inoculated a 13C-labelled pollutant-degrading consortium in these soils and tracked the fate of the labelled biomass through stable isotope probing (SIP) of DNA. We identified active bacterial and eukaryotic inoculum-biomass consumers through amplicon sequencing of 16S rRNA and 18S rRNA genes coupled to a novel enrichment factor calculation. Results Inoculation effectively increased PAH removal in the short-term, but not in the long-term polluted soil. A decrease in the relative abundance of the inoculated genera was observed already on day 15 in the long-term polluted soil, while growth of these genera was observed in the short-term polluted soil, indicating establishment of the inoculum. In both soils, eukaryotic genera dominated as early incorporators of 13C-labelled biomass, while bacteria incorporated the labelled biomass at the end of the incubation period, probably through cross-feeding. We also found different successional patterns between the two soils. In the short-term polluted soil, Cercozoa and Fungi genera predominated as early incorporators, whereas Ciliophora, Ochrophyta and Amoebozoa were the predominant genera in the long-term polluted soil. Conclusion Our results showed differences in the inoculum establishment and predator community responses, affecting bioaugmentation efficiency. This highlights the need to further study predation effects on inoculum survival to increase the applicability of inoculation-based technologies.
Druh dokumentu: Article
Other literature type
Popis súboru: application/pdf
Jazyk: English
ISSN: 2049-2618
DOI: 10.1186/s40168-024-01865-2
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39113100
https://doaj.org/article/e1c21a38128e428f9aa94e1e65303e05
http://hdl.handle.net/11336/264523
https://repository.publisso.de/resource/frl:6524044
Rights: CC BY
URL: http://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (http://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Prístupové číslo: edsair.doi.dedup.....29a60ba885ec9aec74aa74989283011d
Databáza: OpenAIRE
Popis
Abstrakt:Background Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable. Predation is a key top-down control mechanism affecting inoculum establishment, however, its effects on this process have received little attention. This study focused on the impact of trophic interactions on bioaugmentation success in two soils with different pollution exposure histories. We inoculated a 13C-labelled pollutant-degrading consortium in these soils and tracked the fate of the labelled biomass through stable isotope probing (SIP) of DNA. We identified active bacterial and eukaryotic inoculum-biomass consumers through amplicon sequencing of 16S rRNA and 18S rRNA genes coupled to a novel enrichment factor calculation. Results Inoculation effectively increased PAH removal in the short-term, but not in the long-term polluted soil. A decrease in the relative abundance of the inoculated genera was observed already on day 15 in the long-term polluted soil, while growth of these genera was observed in the short-term polluted soil, indicating establishment of the inoculum. In both soils, eukaryotic genera dominated as early incorporators of 13C-labelled biomass, while bacteria incorporated the labelled biomass at the end of the incubation period, probably through cross-feeding. We also found different successional patterns between the two soils. In the short-term polluted soil, Cercozoa and Fungi genera predominated as early incorporators, whereas Ciliophora, Ochrophyta and Amoebozoa were the predominant genera in the long-term polluted soil. Conclusion Our results showed differences in the inoculum establishment and predator community responses, affecting bioaugmentation efficiency. This highlights the need to further study predation effects on inoculum survival to increase the applicability of inoculation-based technologies.
ISSN:20492618
DOI:10.1186/s40168-024-01865-2