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Hyperspectral imaging of human liver allografts for prediction of initial graft function

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Názov: Hyperspectral imaging of human liver allografts for prediction of initial graft function
Autori: Franziska Vogt, Tristan Wagner, Shadi Katou, Felicia Kneifel, Thomas Vogel, Haluk Morgül, Philipp Houben, Philip Wahl, Andreas Pascher, Sonia Radunz
Zdroj: Langenbecks Arch Surg
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2024.
Rok vydania: 2024
Predmety: Male, Adult, Research, Graft Survival, Medizin, Liver transplantation, Female [MeSH], Allografts [MeSH], Organ Preservation/methods [MeSH], Aged [MeSH], Adult [MeSH], Humans [MeSH], Predictive Value of Tests [MeSH], Liver Transplantation [MeSH], Middle Aged [MeSH], Reperfusion Injury [MeSH], Normothermic machine perfusion, Graft Survival [MeSH], Human, Male [MeSH], Early allograft dysfunction, Hyperspectral Imaging/methods [MeSH], Hyperspectral imaging, Organ Preservation, Hyperspectral Imaging, Middle Aged, Allografts, Liver Transplantation, Predictive Value of Tests, Reperfusion Injury, Humans, Female, Aged
Popis: Purpose Ischemia reperfusion injury represents a significant yet difficult to assess risk factor for short- and long-term graft impairment in human liver transplantation (LT). As a non-invasive, non-ionizing tool, hyperspectral imaging (HSI) is capable of correlating optical properties with organ microperfusion. Hence, we here performed a study of human liver allografts assessed by HSI for microperfusion and prediction of initial graft function. Methods Images of liver parenchyma of 37 human liver allografts were acquired at bench preparation, during normothermic machine perfusion (NMP), if applicable, and after reperfusion in the recipient. A specialized HSI acquisition software computed oxygen saturation (StO2), tissue hemoglobin indices (THI), near infrared perfusion indices (NIR), and tissue water indices (TWI). HSI parameters were analyzed for differences with regard to preservation technique, reperfusion sequence and presence of early allograft dysfunction (EAD). Results Organ preservation was performed by means of NMP (n = 31) or static cold storage (SCS; n = 6). Patients’ demographics, donor characteristics, presence of EAD (NMP 36.7% vs. SCS 50%, p = 0.6582), and HSI parameters were comparable between both groups of preservation method. In organs developing EAD, NIR at 1, 2, and 4 h NMP and after reperfusion in the recipient was significantly lower (1 h NMP: 18.6 [8.6–27.6] vs. 28.3 [22.5–39.4], p = 0.0468; 2 h NMP: 19.4 [8.7–30.4] vs. 37.1 [27.5–44.6], p = 0.0011; 4 h NMP: 26.0 [6.8–37.1] vs. 40.3 [32.3–49.9], p = 0.0080; reperfusion: 13.0 [11.5–34.3] vs. 30.6 [19.3–44.0], p = 0.0212). Conclusion HSI assessment of human liver allografts is feasible during organ preservation and in the recipient. NIR during NMP and after reperfusion might predict the onset of EAD. Larger trials are warranted for assessment of this novel technique in human LT.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 1435-2451
DOI: 10.1007/s00423-024-03497-4
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39400566
https://repository.publisso.de/resource/frl:6524436
Rights: CC BY
Prístupové číslo: edsair.doi.dedup.....274fb60ea08a7af9bbd2fe70f2eb85cf
Databáza: OpenAIRE
Popis
Abstrakt:Purpose Ischemia reperfusion injury represents a significant yet difficult to assess risk factor for short- and long-term graft impairment in human liver transplantation (LT). As a non-invasive, non-ionizing tool, hyperspectral imaging (HSI) is capable of correlating optical properties with organ microperfusion. Hence, we here performed a study of human liver allografts assessed by HSI for microperfusion and prediction of initial graft function. Methods Images of liver parenchyma of 37 human liver allografts were acquired at bench preparation, during normothermic machine perfusion (NMP), if applicable, and after reperfusion in the recipient. A specialized HSI acquisition software computed oxygen saturation (StO2), tissue hemoglobin indices (THI), near infrared perfusion indices (NIR), and tissue water indices (TWI). HSI parameters were analyzed for differences with regard to preservation technique, reperfusion sequence and presence of early allograft dysfunction (EAD). Results Organ preservation was performed by means of NMP (n = 31) or static cold storage (SCS; n = 6). Patients’ demographics, donor characteristics, presence of EAD (NMP 36.7% vs. SCS 50%, p = 0.6582), and HSI parameters were comparable between both groups of preservation method. In organs developing EAD, NIR at 1, 2, and 4 h NMP and after reperfusion in the recipient was significantly lower (1 h NMP: 18.6 [8.6–27.6] vs. 28.3 [22.5–39.4], p = 0.0468; 2 h NMP: 19.4 [8.7–30.4] vs. 37.1 [27.5–44.6], p = 0.0011; 4 h NMP: 26.0 [6.8–37.1] vs. 40.3 [32.3–49.9], p = 0.0080; reperfusion: 13.0 [11.5–34.3] vs. 30.6 [19.3–44.0], p = 0.0212). Conclusion HSI assessment of human liver allografts is feasible during organ preservation and in the recipient. NIR during NMP and after reperfusion might predict the onset of EAD. Larger trials are warranted for assessment of this novel technique in human LT.
ISSN:14352451
DOI:10.1007/s00423-024-03497-4