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Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

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Title: Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus
Authors: Kumar, Neeraj, Gismondi, Eric, Reddy, Kotha Sammi
Contributors: SERB - Science and Engineering Research Board
Source: Environmental Toxicology. 39:1581-1600
Publisher Information: Wiley, 2023.
Publication Year: 2023
Subject Terms: Gills, Cellulars, Sciences de l'environnement & écologie, Health, Toxicology and Mutagenesis, Management, Monitoring, Policy and Law, Biomarkers/metabolism, Toxicology, IBR, Environmental sciences & ecology, Water Pollutants, Chemical/toxicity, oxidative stress, Animals, Liver tissue, Acetylcholinesterase/metabolism, Integrated biomarker response, Catfishes, fish, acute test, Catalase/metabolism, Kidney tissues, copper nanoparticles, General Medicine, Catalase, Copper/toxicity, Life sciences, Gills/metabolism, 3. Good health, Oxidative Stress, Gill tissues, Sciences du vivant, Acetylcholinesterase, Cu nano-particles, Copper, Biomarkers, Water Pollutants, Chemical, neurotransmitter
Description: The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu‐NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu‐NPs were determined as 8.04 and 3.85 mg L−1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L−1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L−1 for Cu‐NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione‐s‐transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu‐NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu‐NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu‐NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu‐NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu‐NPs, induce toxicity and disrupt cellular metabolic activities in fish.
Document Type: Article
Language: English
ISSN: 1522-7278
1520-4081
DOI: 10.1002/tox.24058
Access URL: https://pubmed.ncbi.nlm.nih.gov/38009665
Rights: Wiley Online Library User Agreement
Accession Number: edsair.doi.dedup.....251493d01e8c54eeed156d7323f7b5d0
Database: OpenAIRE
Description
Abstract:The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu‐NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu‐NPs were determined as 8.04 and 3.85 mg L−1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L−1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L−1 for Cu‐NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione‐s‐transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu‐NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu‐NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu‐NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu‐NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu‐NPs, induce toxicity and disrupt cellular metabolic activities in fish.
ISSN:15227278
15204081
DOI:10.1002/tox.24058