Zobraziť v EDS

Complete genome sequence of a novel iflavirus from wheat sawfly (Dolerus tritici)

Uložené v:
Podrobná bibliografia
Názov: Complete genome sequence of a novel iflavirus from wheat sawfly (Dolerus tritici)
Autori: Guo, Jiashu, Liu, Wenwen, Chen, Chen, Xu, Zhongtian, Francis, Frédéric, Wang, Xifeng
Prispievatelia: NSCF - National Natural Science Foundation of China
Zdroj: Archives of Virology. 170
Informácie o vydavateľovi: Springer Science and Business Media LLC, 2024.
Rok vydania: 2024
Predmety: RNA Viruses/classification, China, Insect Viruses/genetics, RNA Viruses/genetics, Insect Viruses, Genome, Viral, RNA Viruses/isolation & purification, Viral Proteins, Open Reading Frames, Agriculture & agronomie, Virology, Animals, RNA Viruses, Amino Acid Sequence, Phylogeny, Triticum, Insect Viruses/classification, Whole Genome Sequencing, Base Sequence, Insect Viruses/isolation & purification, Triticum/virology, Agriculture & agronomy, Life sciences, Hymenoptera, RNA, Viral/genetics, Hymenoptera/virology, Larva, Viral Proteins/genetics, Sciences du vivant, RNA, Viral, Larva/virology
Popis: Little is known about the insect viruses in wheat sawfly, Dolerus tritici, which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3' terminus, and it is predicted to encode a 326.3-kDa polyprotein. Phylogenetic analysis based on deduced amino acid sequences of the polyprotein revealed that this RNA virus clustered in a clade with deformed wing virus of the genus Iflavirus, family Iflaviridae. The full genome of this RNA virus shows 42.0-50.0% sequence identity with other iflaviruses. Comparisons of amino acid sequences showed that the coat protein of this RNA virus is most similar to that of slow bee paralysis virus, with 33.6% identity, suggesting that this virus is a new member in the genus Iflavirus. Thus, we have tentatively designated it as "Dolerus tritici iflavirus 1" (DtIV1). To our knowledge, this is the first report of an insect virus in wheat sawfly.
Druh dokumentu: Article
Jazyk: English
ISSN: 1432-8798
0304-8608
DOI: 10.1007/s00705-024-06206-0
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39690328
Rights: Springer Nature TDM
Prístupové číslo: edsair.doi.dedup.....248bef842883e00a4868926c114e7d6b
Databáza: OpenAIRE
Popis
Abstrakt:Little is known about the insect viruses in wheat sawfly, Dolerus tritici, which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3' terminus, and it is predicted to encode a 326.3-kDa polyprotein. Phylogenetic analysis based on deduced amino acid sequences of the polyprotein revealed that this RNA virus clustered in a clade with deformed wing virus of the genus Iflavirus, family Iflaviridae. The full genome of this RNA virus shows 42.0-50.0% sequence identity with other iflaviruses. Comparisons of amino acid sequences showed that the coat protein of this RNA virus is most similar to that of slow bee paralysis virus, with 33.6% identity, suggesting that this virus is a new member in the genus Iflavirus. Thus, we have tentatively designated it as "Dolerus tritici iflavirus 1" (DtIV1). To our knowledge, this is the first report of an insect virus in wheat sawfly.
ISSN:14328798
03048608
DOI:10.1007/s00705-024-06206-0