The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans
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| Názov: | The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans |
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| Autori: | Anna Gavrilova, Astrid Boström, Nickolay Korabel, Sergei Fedotov, Gino B. Poulin, Victoria J. Allan |
| Zdroj: | J Cell Sci Gavrilova, A, Boström, A, Korabel, N, Fedotov, S, Poulin, G B & Allan, V J 2024, 'The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans', Journal of Cell Science, vol. 137, no. 17, jcs262148. https://doi.org/10.1242/jcs.262148 |
| Informácie o vydavateľovi: | The Company of Biologists, 2024. |
| Rok vydania: | 2024 |
| Predmety: | 0301 basic medicine, Kinesins/metabolism, Mutation/genetics, Longevity, Neurons/metabolism, Neuromuscular Junction, Kinesins, Genetically Modified, Cell Cycle Proteins, Axonal Transport, Animals, Genetically Modified, 03 medical and health sciences, Animals, Neuromuscular Junction/metabolism, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Secretory Vesicles/metabolism, Neurons, Caenorhabditis elegans/metabolism, 0303 health sciences, Secretory Vesicles, Longevity/genetics, Locomotion/genetics, Mutation, Caenorhabditis elegans Proteins/metabolism, Locomotion, Research Article |
| Popis: | Fast axonal transport is crucial for neuronal function and is driven by kinesins and cytoplasmic dynein. Here, we investigated the role of kinesin-1 in dense core vesicle (DCV) transport in C. elegans, using mutants in the kinesin light chains (klc-1 and klc-2) and the motor subunit (unc-116) expressing an ida-1::gfp transgene that labels DCVs. DCV transport in both directions was greatly impaired in an unc-116 mutant and had reduced velocity in a klc-2 mutant. In contrast, the speed of retrograde DCV transport was increased in a klc-1 mutant whereas anterograde transport was unaffected. We identified striking differences between the klc mutants in their effects on worm locomotion and responses to drugs affecting neuromuscular junction activity. We also determined lifespan, finding that unc-116 mutant was short-lived whereas the klc single mutant lifespan was wild type. The ida-1::gfp transgenic strain was also short-lived, but surprisingly, klc-1 and klc-2 extended the ida-1::gfp lifespan beyond that of wild type. Our findings suggest that kinesin-1 not only influences anterograde and retrograde DCV transport but is also involved in regulating lifespan and locomotion, with the two kinesin light chains playing distinct roles. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.262148 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/39171448 http://www.scopus.com/inward/record.url?scp=85203476124&partnerID=8YFLogxK https://doi.org/10.1242/jcs.262148 https://research.manchester.ac.uk/en/publications/df87d63e-cfc6-4c80-8d2a-a45a1d871f7a |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0 (http://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| Prístupové číslo: | edsair.doi.dedup.....22e429fb2548d5ca8afb3f66d33f5712 |
| Databáza: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Fast axonal transport is crucial for neuronal function and is driven by kinesins and cytoplasmic dynein. Here, we investigated the role of kinesin-1 in dense core vesicle (DCV) transport in C. elegans, using mutants in the kinesin light chains (klc-1 and klc-2) and the motor subunit (unc-116) expressing an ida-1::gfp transgene that labels DCVs. DCV transport in both directions was greatly impaired in an unc-116 mutant and had reduced velocity in a klc-2 mutant. In contrast, the speed of retrograde DCV transport was increased in a klc-1 mutant whereas anterograde transport was unaffected. We identified striking differences between the klc mutants in their effects on worm locomotion and responses to drugs affecting neuromuscular junction activity. We also determined lifespan, finding that unc-116 mutant was short-lived whereas the klc single mutant lifespan was wild type. The ida-1::gfp transgenic strain was also short-lived, but surprisingly, klc-1 and klc-2 extended the ida-1::gfp lifespan beyond that of wild type. Our findings suggest that kinesin-1 not only influences anterograde and retrograde DCV transport but is also involved in regulating lifespan and locomotion, with the two kinesin light chains playing distinct roles. |
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| ISSN: | 14779137 00219533 |
| DOI: | 10.1242/jcs.262148 |