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Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury

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Title: Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury
Authors: Bohnert, Simone, Wirth, Christoph, Schmitz, Werner, Trella, Stefanie, Monoranu, Camelia-Maria, Ondruschka, Benjamin, Bohnert, Michael
Source: Int J Legal Med
Publisher Information: Springer Science and Business Media LLC, 2021.
Publication Year: 2021
Subject Terms: Adult, Aged, 80 and over, Male, ddc:610, Enzyme-Linked Immunosorbent Assay, Myelin Basic Protein, Middle Aged, Female [MeSH], Neurofilament Proteins/cerebrospinal fluid [MeSH], Aged, 80 and over [MeSH], Aged [MeSH], Adult [MeSH], Humans [MeSH], Cerebrospinal fluid, Middle Aged [MeSH], Biomarker, Original Article, Myelin Basic Protein/cerebrospinal fluid [MeSH], Biomarkers/cerebrospinal fluid [MeSH], Male [MeSH], CSF, Brain Injuries, Traumatic/diagnosis [MeSH], Case-Control Studies [MeSH], Enzyme-Linked Immunosorbent Assay [MeSH], Biofluid, Forensic neurotraumatology, Forensic neuropathology, Immunohistochemistry/methods [MeSH], Autopsy [MeSH], Immunohistochemistry, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, Neurofilament Proteins, Case-Control Studies, Brain Injuries, Traumatic, Humans, Female, Autopsy, Biomarkers, Aged
Description: The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as usefulneuroinjurybiomarkers of traumatic brain injury.
Document Type: Article
Other literature type
File Description: application/pdf
Language: English
ISSN: 1437-1596
0937-9827
DOI: 10.1007/s00414-021-02606-y
Access URL: https://link.springer.com/content/pdf/10.1007/s00414-021-02606-y.pdf
https://pubmed.ncbi.nlm.nih.gov/33895854
https://www.ncbi.nlm.nih.gov/pubmed/33895854
https://link.springer.com/article/10.1007/s00414-021-02606-y
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205912
https://link.springer.com/content/pdf/10.1007/s00414-021-02606-y.pdf
https://europepmc.org/article/MED/33895854
https://repository.publisso.de/resource/frl:6447148
https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/26692
https://opus.bibliothek.uni-wuerzburg.de/files/26692/Bohnert_International.pdf
https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-266929
https://doi.org/10.1007/s00414-021-02606-y
Rights: CC BY
URL: http://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) .
Accession Number: edsair.doi.dedup.....2109d5cc09514f0bcd341344d614e554
Database: OpenAIRE
Description
Abstract:The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as usefulneuroinjurybiomarkers of traumatic brain injury.
ISSN:14371596
09379827
DOI:10.1007/s00414-021-02606-y