A missense mutation in the barley Xan-h gene encoding the Mg-chelatase subunit I leads to a viable pale green line with reduced daily transpiration rate
Saved in:
| Title: | A missense mutation in the barley Xan-h gene encoding the Mg-chelatase subunit I leads to a viable pale green line with reduced daily transpiration rate |
|---|---|
| Authors: | Persello, Andrea, Tadini, Luca, Rotasperti, Lisa, Ballabio, Federico, Tagliani, Andrea, Torricella, Viola, Jahns, Peter, Dalal, Ahan, Moshelion, Menachem, Camilloni, Carlo, Rosignoli, Serena, Hansson, Mats, Cattivelli, Luigi, Horner, David S., Rossini, Laura, Tondelli, Alessandro, Salvi, Silvio, Pesaresi, Paolo |
| Source: | Plant Cell Rep Plant Cell Reports |
| Publisher Information: | Springer Science and Business Media LLC, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | Chlorophyll, Plant Leaves, Canopy photosynthesis, Pale green leaves, Chlorophyll biosynthesis, Mg-chelatase, Drought stress, Phenotype, Barley, Mutation, Missense, Lyases, Photosystem II Protein Complex, Original Article, Hordeum, Plant Transpiration, Photosynthesis, Plant Proteins |
| Description: | Key message The barley mutant xan-h.chli-1 shows phenotypic features, such as reduced leaf chlorophyll content and daily transpiration rate, typical of wild barley accessions and landraces adapted to arid climatic conditions. Abstract The pale green trait, i.e. reduced chlorophyll content, has been shown to increase the efficiency of photosynthesis and biomass accumulation when photosynthetic microorganisms and tobacco plants are cultivated at high densities. Here, we assess the effects of reducing leaf chlorophyll content in barley by altering the chlorophyll biosynthesis pathway (CBP). To this end, we have isolated and characterised the pale green barley mutant xan-h.chli-1, which carries a missense mutation in the Xan-h gene for subunit I of Mg-chelatase (HvCHLI), the first enzyme in the CBP. Intriguingly, xan-h.chli-1 is the only known viable homozygous mutant at the Xan-h locus in barley. The Arg298Lys amino-acid substitution in the ATP-binding cleft causes a slight decrease in HvCHLI protein abundance and a marked reduction in Mg-chelatase activity. Under controlled growth conditions, mutant plants display reduced accumulation of antenna and photosystem core subunits, together with reduced photosystem II yield relative to wild-type under moderate illumination, and consistently higher than wild-type levels at high light intensities. Moreover, the reduced content of leaf chlorophyll is associated with a stable reduction in daily transpiration rate, and slight decreases in total biomass accumulation and water-use efficiency, reminiscent of phenotypic features of wild barley accessions and landraces that thrive under arid climatic conditions. |
| Document Type: | Article Other literature type |
| File Description: | application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| Language: | English |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-024-03328-2 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/39343835 https://hdl.handle.net/11585/1013191 https://link.springer.com/article/10.1007/s00299-024-03328-2 https://doi.org/10.1007/s00299-024-03328-2 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....20b3a8501a7e6c18cb3edcdb313eceb8 |
| Database: | OpenAIRE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstract: | Key message The barley mutant xan-h.chli-1 shows phenotypic features, such as reduced leaf chlorophyll content and daily transpiration rate, typical of wild barley accessions and landraces adapted to arid climatic conditions. Abstract The pale green trait, i.e. reduced chlorophyll content, has been shown to increase the efficiency of photosynthesis and biomass accumulation when photosynthetic microorganisms and tobacco plants are cultivated at high densities. Here, we assess the effects of reducing leaf chlorophyll content in barley by altering the chlorophyll biosynthesis pathway (CBP). To this end, we have isolated and characterised the pale green barley mutant xan-h.chli-1, which carries a missense mutation in the Xan-h gene for subunit I of Mg-chelatase (HvCHLI), the first enzyme in the CBP. Intriguingly, xan-h.chli-1 is the only known viable homozygous mutant at the Xan-h locus in barley. The Arg298Lys amino-acid substitution in the ATP-binding cleft causes a slight decrease in HvCHLI protein abundance and a marked reduction in Mg-chelatase activity. Under controlled growth conditions, mutant plants display reduced accumulation of antenna and photosystem core subunits, together with reduced photosystem II yield relative to wild-type under moderate illumination, and consistently higher than wild-type levels at high light intensities. Moreover, the reduced content of leaf chlorophyll is associated with a stable reduction in daily transpiration rate, and slight decreases in total biomass accumulation and water-use efficiency, reminiscent of phenotypic features of wild barley accessions and landraces that thrive under arid climatic conditions. |
|---|---|
| ISSN: | 1432203X 07217714 |
| DOI: | 10.1007/s00299-024-03328-2 |