A novel Microbacterium strain SRS2 promotes the growth of Arabidopsis and MicroTom (S. lycopersicum) under normal and salt stress conditions
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| Titel: | A novel Microbacterium strain SRS2 promotes the growth of Arabidopsis and MicroTom (S. lycopersicum) under normal and salt stress conditions |
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| Autoren: | Ho Manh Tuong, Sonia García Méndez, Michiel Vandecasteele, Anne Willems, Anelia Iancheva, Pham Bich Ngoc, Do Tien Phat, Chu Hoang Ha, Sofie Goormachtig |
| Quelle: | PLANTA |
| Verlagsinformationen: | Springer Science and Business Media LLC, 2024. |
| Publikationsjahr: | 2024 |
| Schlagwörter: | CHLOROPHYLL BIOSYNTHESIS, Microbacterium, Arabidopsis, Sodium Chloride, Salt Stress, Plant Roots, SIGNAL-TRANSDUCTION PATHWAY, Abscisic acid, Solanum lycopersicum, Gene Expression Regulation, Plant, ABIOTIC STRESS, Salt tolerance, TOLERANCE, DROUGHT, Mutant, Biology and Life Sciences, qRT-PCR, Genomics, Salt Tolerance, ABSCISIC-ACID, OSMOTIC-STRESS, TRANSCRIPTION FACTORS, ABA, PGPR, Reactive Oxygen Species, SALINITY STRESS, Abscisic Acid |
| Beschreibung: | Microbacterium strain SRS2 promotes growth and induces salt stress resistance in Arabidopsis and MicroTom in various growth substrates via the induction of the ABA pathway. Soil salinity reduces plant growth and development and thereby decreases the value and productivity of soils. Plant growth-promoting rhizobacteria (PGPR) have been shown to support plant growth such as in salt stress conditions. Here, Microbacterium strain SRS2, isolated from the root endosphere of tomato, was tested for its capability to help plants cope with salt stress. In a salt tolerance assay, SRS2 grew well up to medium levels of NaCl, but the growth was inhibited at high salt concentrations. SRS2 inoculation led to increased biomass of Arabidopsis and MicroTom tomato in various growth substrates, in the presence and in the absence of high NaCl concentrations. Whole-genome analysis revealed that the strain contains several genes involved in osmoregulation and reactive oxygen species (ROS) scavenging, which could potentially explain the observed growth promotion. Additionally, we also investigated via qRT-PCR, promoter::GUS and mutant analyses whether the abscisic acid (ABA)-dependent or -independent pathways for tolerance against salt stress were involved in the model plant, Arabidopsis. Especially in salt stress conditions, the plant growth-promotion effect of SRS2 was lost in aba1, abi4-102, abi3, and abi5-1 mutant lines. Furthermore, ABA genes related to salt stress in SRS2-inoculated plants were transiently upregulated compared to mock under salt stress conditions. Additionally, SRS2-inoculated ABI4::GUS and ABI5::GUS plants were slightly more activated compared to the uninoculated control under salt stress conditions. Together, these assays show that SRS2 promotes growth in normal and in salt stress conditions, the latter possibly via the induction of ABA-dependent and -independent pathways. |
| Publikationsart: | Article |
| Dateibeschreibung: | application/pdf |
| Sprache: | English |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-024-04510-2 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/39182196 https://biblio.ugent.be/publication/01J7B4B1D1NMNR12D9CG17NERP http://hdl.handle.net/1854/LU-01J7B4B1D1NMNR12D9CG17NERP https://biblio.ugent.be/publication/01J7B4B1D1NMNR12D9CG17NERP/file/01J7B4VXTQC7B568NZ8QGANH0S http://doi.org/10.1007/s00425-024-04510-2 |
| Rights: | Springer Nature TDM |
| Dokumentencode: | edsair.doi.dedup.....20b0e7a4328c868df89ba88a4ab273f0 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Microbacterium strain SRS2 promotes growth and induces salt stress resistance in Arabidopsis and MicroTom in various growth substrates via the induction of the ABA pathway. Soil salinity reduces plant growth and development and thereby decreases the value and productivity of soils. Plant growth-promoting rhizobacteria (PGPR) have been shown to support plant growth such as in salt stress conditions. Here, Microbacterium strain SRS2, isolated from the root endosphere of tomato, was tested for its capability to help plants cope with salt stress. In a salt tolerance assay, SRS2 grew well up to medium levels of NaCl, but the growth was inhibited at high salt concentrations. SRS2 inoculation led to increased biomass of Arabidopsis and MicroTom tomato in various growth substrates, in the presence and in the absence of high NaCl concentrations. Whole-genome analysis revealed that the strain contains several genes involved in osmoregulation and reactive oxygen species (ROS) scavenging, which could potentially explain the observed growth promotion. Additionally, we also investigated via qRT-PCR, promoter::GUS and mutant analyses whether the abscisic acid (ABA)-dependent or -independent pathways for tolerance against salt stress were involved in the model plant, Arabidopsis. Especially in salt stress conditions, the plant growth-promotion effect of SRS2 was lost in aba1, abi4-102, abi3, and abi5-1 mutant lines. Furthermore, ABA genes related to salt stress in SRS2-inoculated plants were transiently upregulated compared to mock under salt stress conditions. Additionally, SRS2-inoculated ABI4::GUS and ABI5::GUS plants were slightly more activated compared to the uninoculated control under salt stress conditions. Together, these assays show that SRS2 promotes growth in normal and in salt stress conditions, the latter possibly via the induction of ABA-dependent and -independent pathways. |
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| ISSN: | 14322048 00320935 |
| DOI: | 10.1007/s00425-024-04510-2 |