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Beam narrowing test: a motor index of post-stroke motor evaluation in an aged rat model of cerebral ischemia

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Bibliographic Details
Title: Beam narrowing test: a motor index of post-stroke motor evaluation in an aged rat model of cerebral ischemia
Authors: Leonard Radu Pinosanu, Ianis Kevyn Stefan Boboc, Tudor Adrian Balseanu, Andrei Gresita, Dirk M. Hermann, Aurel Popa‐Wagner, Bogdan Catalin
Source: J Neural Transm (Vienna)
Publisher Information: Springer Science and Business Media LLC, 2024.
Publication Year: 2024
Subject Terms: Male, 0301 basic medicine, Aging, Medizin, Recovery of Function, Motor Activity, Neurology and Preclinical Neurological Studies - Original Article, Brain Ischemia, Rats, Rats, Sprague-Dawley, Stroke, Disease Models, Animal, 03 medical and health sciences, 0302 clinical medicine, Recovery of Function/physiology [MeSH], Rats [MeSH], Rats, Sprague-Dawley [MeSH], Stroke/complications [MeSH], Animals [MeSH], Brain Ischemia/complications [MeSH], Motor Activity/physiology [MeSH], Behaviour, Male [MeSH], Beam narrowing test, Brain Ischemia/physiopathology [MeSH], Stroke/physiopathology [MeSH], Motor performance index, Aging/physiology [MeSH], Disease Models, Animal [MeSH], Animals
Description: Each year, 15 million people worldwide suffer from strokes. Consequently, researchers face increasing pressure to develop reliable behavioural tests for assessing functional recovery after a stroke. Our aim was to establish a new motor performance index that can be used to evaluate post-stroke recovery in both young and aged animals. Furthermore, we validate the proposed procedure and recommend the necessary number of animals for experimental stroke studies. Young (n = 20) and aged (n = 27) Sprague–Dawley rats were randomly assigned to receive either sham or stroke surgery. The newly proposed performance index was calculated for the post-stroke acute, subacute and chronic phases. The advantage of using our test over current tests lies in the fact that the newly proposed motor index test evaluates not only the performance of the unaffected side in comparison to the affected one but also assesses overall performance by taking into account speed and coordination. Moreover, it reduces the number of animals needed to achieve a statistical power of 80%. This aspect is particularly crucial when studying aged rodents. Our approach can be used to monitor and assess the effectiveness of stroke therapies in experimental models using aged animals.
Document Type: Article
Other literature type
Language: English
ISSN: 1435-1463
0300-9564
DOI: 10.1007/s00702-024-02768-0
Access URL: https://pubmed.ncbi.nlm.nih.gov/38598100
https://repository.publisso.de/resource/frl:6522494
Rights: CC BY
Accession Number: edsair.doi.dedup.....20847c37f56654a9b334f2c748a8a390
Database: OpenAIRE
Description
Abstract:Each year, 15 million people worldwide suffer from strokes. Consequently, researchers face increasing pressure to develop reliable behavioural tests for assessing functional recovery after a stroke. Our aim was to establish a new motor performance index that can be used to evaluate post-stroke recovery in both young and aged animals. Furthermore, we validate the proposed procedure and recommend the necessary number of animals for experimental stroke studies. Young (n = 20) and aged (n = 27) Sprague–Dawley rats were randomly assigned to receive either sham or stroke surgery. The newly proposed performance index was calculated for the post-stroke acute, subacute and chronic phases. The advantage of using our test over current tests lies in the fact that the newly proposed motor index test evaluates not only the performance of the unaffected side in comparison to the affected one but also assesses overall performance by taking into account speed and coordination. Moreover, it reduces the number of animals needed to achieve a statistical power of 80%. This aspect is particularly crucial when studying aged rodents. Our approach can be used to monitor and assess the effectiveness of stroke therapies in experimental models using aged animals.
ISSN:14351463
03009564
DOI:10.1007/s00702-024-02768-0