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(Un)Reliable detection of menstrual blood in forensic casework — evaluation of the Seratec® PMB test with mock samples

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Název: (Un)Reliable detection of menstrual blood in forensic casework — evaluation of the Seratec® PMB test with mock samples
Autoři: Helen Konrad, Benno Hartung, Micaela Poetsch
Zdroj: Int J Legal Med
Informace o vydavateli: Springer Science and Business Media LLC, 2023.
Rok vydání: 2023
Témata: Male, Forensic Genetics, Bodily Secretions, Hemoglobins, Rapid test, Stain identification, Humans [MeSH], Hemoglobins/analysis [MeSH], Body Fluids/chemistry [MeSH], Immunochromatographic lateral flow strip test, Menstrual blood, Forensic Genetics/methods [MeSH], Male [MeSH], Semen/chemistry [MeSH], Body fluids, Saliva/chemistry [MeSH], Short Communication, Bodily Secretions/chemistry [MeSH], Semen, Medizin, Humans, Saliva, 3. Good health, Body Fluids
Popis: The identification of the type of body fluid in crime scene evidence may be crucial, so that the efforts are high to reduce the complexity of these analyses and to minimize time and costs. Reliable immunochromatographic rapid tests for specific and sensitive identification of blood, saliva, urine and sperm secretions are already routinely used in forensic genetics. The recently introduced Seratec® PMB test is said to detect not only hemoglobin, but also differentiate menstrual blood from other secretions containing blood (cells) by detecting D-dimers. In our experimental set-up, menstrual blood could be reliably detected in mock forensic samples. Here, the result was independent of sample age and extraction buffer volume. It was also successfully demonstrated that all secretions without blood cells were negative for both, hemoglobin (P) and D-dimer (M). However, several blood cell–containing secretions/tissues comprising blood (injury), nasal blood, postmortem blood and wound crust also demonstrated positive results for D-dimer (M) and were therefore false positives. For blood (injury) and nasal blood, this result was reproduced for different extraction buffer volumes. The results of this study clearly demonstrate that the Seratec® PMB test is neither useful nor suitable for use in forensic genetics because of the great risk of false positive results which can lead to false conclusions, especially in sexual offense or violent acts.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 1437-1596
0937-9827
DOI: 10.1007/s00414-023-03138-3
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/38030939
https://repository.publisso.de/resource/frl:6521919
https://www.ncbi.nlm.nih.gov/pubmed/38030939
https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85178189458
https://doi.org/10.1007/s00414-023-03138-3
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....1e9f2144a33a278be445981880bab82c
Databáze: OpenAIRE
Popis
Abstrakt:The identification of the type of body fluid in crime scene evidence may be crucial, so that the efforts are high to reduce the complexity of these analyses and to minimize time and costs. Reliable immunochromatographic rapid tests for specific and sensitive identification of blood, saliva, urine and sperm secretions are already routinely used in forensic genetics. The recently introduced Seratec® PMB test is said to detect not only hemoglobin, but also differentiate menstrual blood from other secretions containing blood (cells) by detecting D-dimers. In our experimental set-up, menstrual blood could be reliably detected in mock forensic samples. Here, the result was independent of sample age and extraction buffer volume. It was also successfully demonstrated that all secretions without blood cells were negative for both, hemoglobin (P) and D-dimer (M). However, several blood cell–containing secretions/tissues comprising blood (injury), nasal blood, postmortem blood and wound crust also demonstrated positive results for D-dimer (M) and were therefore false positives. For blood (injury) and nasal blood, this result was reproduced for different extraction buffer volumes. The results of this study clearly demonstrate that the Seratec® PMB test is neither useful nor suitable for use in forensic genetics because of the great risk of false positive results which can lead to false conclusions, especially in sexual offense or violent acts.
ISSN:14371596
09379827
DOI:10.1007/s00414-023-03138-3