Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation
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| Název: | Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation |
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| Autoři: | Katarzyna Bielawska, Izabela Dobrzyńska, Elżbieta Skrzydlewska, Agnieszka Gęgotek, Michał Biernacki |
| Zdroj: | Redox Biol Redox Biology, Vol 12, Iss, Pp 733-744 (2017) |
| Informace o vydavateli: | Elsevier BV, 2017. |
| Rok vydání: | 2017 |
| Témata: | Phospholipases A2 - metabolism, 0301 basic medicine, Medicine (General), Time Factors, QH301-705.5, Ultraviolet Rays, Rutin, Cell membrane - metabolism, Fibroblasts - drug effects, Cell Line, Fibroblasts - metabolism, Cell membrane - drug effects, 03 medical and health sciences, R5-920, Cell membrane - radiation effects, Glutathione - metabolism, Humans, Biology (General), Rutin - pharmacology, Phospholipids, Skin, Lipid peroxidation - drug effects, Glutathione Peroxidase, 0303 health sciences, Oxidative stress - radiation effects, Time factors, Cell Membrane, Lipid peroxidation - radiation effects, Skin - drug effects, Skin - radiation effects, Glutathione peroxidase - metabolism, Fibroblasts, Glutathione, Phospholipids - metabolism, Fibroblasts- cytology, Oxidative stress - drug effects, Oxidative Stress, Phospholipases A2, Ultraviolet rays - adverse effects, Lipid Peroxidation, Cell line, Skin - metabolism, Skin - cytology, Research Paper |
| Popis: | Chronic exposure of the skin to solar UV radiation induces a number of biological alterations, including a redox imbalance; therefore, there is an urgent need for skin cells protective compounds. The aim of this study was to determine the effects of natural, previously extensively examined, polyphenol with antioxidant properties - rutin, on UV-induced skin fibroblasts membrane disruption. Accordingly, fibroblasts exposed to UVA and UVB irradiation were incubated with rutin (12h before and/or up to 24h after irradiation), and the structural and metabolic changes were examined. Rutin penetration through the fibroblast phospholipid bilayer was aided by UVA-induced bilitranslocase activity 2-4h after irradiation, while UVB irradiation led to enhanced phospholipid peroxidation and higher membrane permeability to facilitate the interaction of rutin with phospholipids. Lipidomic analysis revealed that 4h of rutin treatment also partially prevented UVA/B-induced increase in phosphatidylethanolamine and phosphatidylcholine level, as well as their membrane localization, which resulted in an enhanced zeta potential in the cells and liposomes. Moreover, rutin 2h following irradiation, in a various degree, prevented the increased in phospholipase A2 activity and ROS generation, and partially protected against the reduction of arachidonic and linoleic acids level and the lipid peroxidation product 4-hydroxynonenal level increase. Rutin effectively prevented against decrease in glutathione peroxidase, glutathione and vitamins E and C activities/levels, particularly 2h following UVA irradiation. In conclusion, highest skin fibroblasts membrane level of rutin occurred in 2-4h following UVA/B-radiation results in its strongest effect on biomembrane structure and functions and cellular antioxidant system irrespective of the radiation type. |
| Druh dokumentu: | Article Other literature type |
| Jazyk: | English |
| ISSN: | 2213-2317 |
| DOI: | 10.1016/j.redox.2017.04.014 |
| Přístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/28412651 https://doaj.org/article/75e45492625a4f6ea78a578e29d8c798 http://europepmc.org/articles/PMC5393167 https://core.ac.uk/display/88845155 https://www.sciencedirect.com/science/article/pii/S2213231717302124 http://www.sciencedirect.com/science/article/pii/S2213231717302124 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393167 https://pubmed.ncbi.nlm.nih.gov/28412651/ http://www.sciencedirect.com/science/article/pii/S2213231717302124 https://doi.org/10.1016/j.redox.2017.04.014 |
| Rights: | CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....1e30b5ce712493c7352de7a0e4d05089 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Chronic exposure of the skin to solar UV radiation induces a number of biological alterations, including a redox imbalance; therefore, there is an urgent need for skin cells protective compounds. The aim of this study was to determine the effects of natural, previously extensively examined, polyphenol with antioxidant properties - rutin, on UV-induced skin fibroblasts membrane disruption. Accordingly, fibroblasts exposed to UVA and UVB irradiation were incubated with rutin (12h before and/or up to 24h after irradiation), and the structural and metabolic changes were examined. Rutin penetration through the fibroblast phospholipid bilayer was aided by UVA-induced bilitranslocase activity 2-4h after irradiation, while UVB irradiation led to enhanced phospholipid peroxidation and higher membrane permeability to facilitate the interaction of rutin with phospholipids. Lipidomic analysis revealed that 4h of rutin treatment also partially prevented UVA/B-induced increase in phosphatidylethanolamine and phosphatidylcholine level, as well as their membrane localization, which resulted in an enhanced zeta potential in the cells and liposomes. Moreover, rutin 2h following irradiation, in a various degree, prevented the increased in phospholipase A2 activity and ROS generation, and partially protected against the reduction of arachidonic and linoleic acids level and the lipid peroxidation product 4-hydroxynonenal level increase. Rutin effectively prevented against decrease in glutathione peroxidase, glutathione and vitamins E and C activities/levels, particularly 2h following UVA irradiation. In conclusion, highest skin fibroblasts membrane level of rutin occurred in 2-4h following UVA/B-radiation results in its strongest effect on biomembrane structure and functions and cellular antioxidant system irrespective of the radiation type. |
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| ISSN: | 22132317 |
| DOI: | 10.1016/j.redox.2017.04.014 |