Effectors of anterior morphogenesis in C. elegans embryos
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| Titel: | Effectors of anterior morphogenesis in C. elegans embryos |
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| Autoren: | Boopathi Balasubramaniam, Irini Topalidou, Melissa Kelley, Sarina M. Meadows, Owen Funk, Michael Ailion, David S. Fay |
| Quelle: | Biol Open Biology Open, Vol 12, Iss 7 (2023) |
| Verlagsinformationen: | The Company of Biologists, 2023. |
| Publikationsjahr: | 2023 |
| Schlagwörter: | QH301-705.5, membrane trafficking, Science, morphogenesis, Embryonic Development, apical extracellular matrix, Extracellular Matrix, caenorhabditis elegans, cell biology, Morphogenesis, Animals, Biology (General), Caenorhabditis elegans, Caenorhabditis elegans Proteins, development, Research Article |
| Beschreibung: | During embryogenesis the nascent Caenorhabditis elegans epidermis secretes an apical extracellular matrix (aECM) that serves as an external stabilizer, preventing deformation of the epidermis by mechanical forces exerted during morphogenesis. At present, the factors that contribute to aECM function are mostly unknown, including the aECM components themselves, their posttranslational regulators, and the pathways required for their secretion. Here we showed that two proteins previously linked to aECM function, SYM-3/FAM102A and SYM-4/WDR44, colocalize to intracellular and membrane-associated puncta and likely function in a complex. Proteomics experiments also suggested potential roles for SYM-3/FAM102A and SYM-4/WDR44 family proteins in intracellular trafficking. Nonetheless, we found no evidence to support a critical function for SYM-3 or SYM-4 in the apical deposition of two aECM components, NOAH-1 and FBN-1. Moreover, loss of a key splicing regulator of fbn-1, MEC-8/RBPMS2, had surprisingly little effect on the abundance or deposition of FBN-1. Using a focused screening approach, we identified 32 additional proteins that likely contribute to the structure and function of the embryonic aECM. We also characterized morphogenesis defects in embryos lacking mir-51 microRNA family members, which display a similar phenotype to mec-8; sym double mutants. Collectively, these findings add to our knowledge of factors controlling embryonic morphogenesis. |
| Publikationsart: | Article Other literature type |
| Sprache: | English |
| ISSN: | 2046-6390 |
| DOI: | 10.1242/bio.059982 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/37345480 https://doaj.org/article/628406337538470ab35089a2a0e0ce90 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0 (http://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| Dokumentencode: | edsair.doi.dedup.....1e0c56798c58d84d78f584d82fd86da6 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | During embryogenesis the nascent Caenorhabditis elegans epidermis secretes an apical extracellular matrix (aECM) that serves as an external stabilizer, preventing deformation of the epidermis by mechanical forces exerted during morphogenesis. At present, the factors that contribute to aECM function are mostly unknown, including the aECM components themselves, their posttranslational regulators, and the pathways required for their secretion. Here we showed that two proteins previously linked to aECM function, SYM-3/FAM102A and SYM-4/WDR44, colocalize to intracellular and membrane-associated puncta and likely function in a complex. Proteomics experiments also suggested potential roles for SYM-3/FAM102A and SYM-4/WDR44 family proteins in intracellular trafficking. Nonetheless, we found no evidence to support a critical function for SYM-3 or SYM-4 in the apical deposition of two aECM components, NOAH-1 and FBN-1. Moreover, loss of a key splicing regulator of fbn-1, MEC-8/RBPMS2, had surprisingly little effect on the abundance or deposition of FBN-1. Using a focused screening approach, we identified 32 additional proteins that likely contribute to the structure and function of the embryonic aECM. We also characterized morphogenesis defects in embryos lacking mir-51 microRNA family members, which display a similar phenotype to mec-8; sym double mutants. Collectively, these findings add to our knowledge of factors controlling embryonic morphogenesis. |
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| ISSN: | 20466390 |
| DOI: | 10.1242/bio.059982 |