2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS): A Narrative Review by the European Calcified Tissue Society (ECTS)
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| Title: | 2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS): A Narrative Review by the European Calcified Tissue Society (ECTS) |
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| Authors: | vom Scheidt, Annika, Krug, Johannes, Goggin, Patricia, Bakker, Astrid Diana, Busse, Björn |
| Source: | Curr Osteoporos Rep |
| Publisher Information: | Springer Science and Business Media LLC, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | 0301 basic medicine, 0303 health sciences, CLSM, Bone Density [MeSH], 3D Imaging, Humans [MeSH], Lacunar volume, Nanoct, Osteocyte, Image Processing, Computer-Assisted/methods [MeSH], Synchrotron, Bone and Bones/diagnostic imaging [MeSH], Bone and Bones/pathology [MeSH], Microct, Review, Imaging, Three-Dimensional/methods [MeSH], Osteocytes/cytology [MeSH], Topical Collection on Skeletal Biology and Regulation, Lacuna, Osteocytes, Bone and Bones, 03 medical and health sciences, Imaging, Three-Dimensional, Bone Density, Image Processing, Computer-Assisted, Humans |
| Description: | Purpose of Review Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2D and 3D imaging methods for the determination of lacunar shape, orientation, density, and volume. Deviations between 2D-based and 3D-based lacunar volume estimations are often not sufficiently addressed and may give rise to contradictory findings. Thus, the systematic error arising from 2D-based estimations of lacunar volume will be discussed, and an alternative calculation proposed. Further, standardized morphological parameters and best practices for sampling and segmentation are suggested. Recent Findings We quantified the errors in reported estimation methods of lacunar volume based on 2D cross-sections, which increase with variations in lacunar orientation and histological cutting plane. The estimations of lacunar volume based on common practice in 2D imaging methods resulted in an underestimation of lacunar volume of up to 85% compared to actual lacunar volume in an artificial dataset. For a representative estimation of lacunar size and morphology based on 2D images, at least 400 lacunae should be assessed per sample. |
| Document Type: | Article Other literature type Review |
| Language: | English |
| ISSN: | 1544-2241 1544-1873 |
| DOI: | 10.1007/s11914-024-00877-z |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38980532 https://hdl.handle.net/1871.1/b5c2d75c-f254-4e27-b945-3dc7d523401f https://research.vu.nl/en/publications/b5c2d75c-f254-4e27-b945-3dc7d523401f https://doi.org/10.1007/s11914-024-00877-z https://repository.publisso.de/resource/frl:6523230 |
| Rights: | CC BY |
| Accession Number: | edsair.doi.dedup.....14036df87eef1d15f2d2d436f95e0e93 |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Purpose of Review Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2D and 3D imaging methods for the determination of lacunar shape, orientation, density, and volume. Deviations between 2D-based and 3D-based lacunar volume estimations are often not sufficiently addressed and may give rise to contradictory findings. Thus, the systematic error arising from 2D-based estimations of lacunar volume will be discussed, and an alternative calculation proposed. Further, standardized morphological parameters and best practices for sampling and segmentation are suggested. Recent Findings We quantified the errors in reported estimation methods of lacunar volume based on 2D cross-sections, which increase with variations in lacunar orientation and histological cutting plane. The estimations of lacunar volume based on common practice in 2D imaging methods resulted in an underestimation of lacunar volume of up to 85% compared to actual lacunar volume in an artificial dataset. For a representative estimation of lacunar size and morphology based on 2D images, at least 400 lacunae should be assessed per sample. |
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| ISSN: | 15442241 15441873 |
| DOI: | 10.1007/s11914-024-00877-z |