Unambiguous Detection of LTR-III G-Quadruplex in the HIV Genome Using a Tailored Fluorogenic Probe-based Assay
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| Titel: | Unambiguous Detection of LTR-III G-Quadruplex in the HIV Genome Using a Tailored Fluorogenic Probe-based Assay |
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| Autoren: | Sumon Pratihar, Vasudhar Bhat S V, Krithi K. Bhagavath, Thimmaiah Govindaraju |
| Quelle: | Analytical Chemistry. 96:15834-15839 |
| Verlagsinformationen: | American Chemical Society (ACS), 2024. |
| Publikationsjahr: | 2024 |
| Schlagwörter: | G-Quadruplexes, Spectrometry, Fluorescence, HIV-1, Humans, HIV, Genome, Viral, Fluorescent Dyes, HIV Long Terminal Repeat |
| Beschreibung: | The noncanonical conformations within the genomes of viral pathogens is of significant diagnostic value, due to their unique secondary structures and interactions with specific fluorogenic molecules. In particular, adaptation of the G-quadruplex (GQ) conformation by the specific gene sequence leads to distinct topological features, resulting in unique binding sites that are crucial for the selective recognition of human immunodeficiency virus (HIV) by small molecules. Leveraging the selective fluorescence response of a benzobisthiazole-based fluorogenic probe to the LTR-III GQ target, we developed a GQ-based diagnostic platform for HIV detection. The successful fluorescence recognition of an amplified 176-nucleotide genomic segment harboring the LTR-III GQ, facilitated by pH-controlled GQ-targeted reliable conformational polymorphism (GQ-RCP), validates this method as an effective GQ-topology-targeted diagnostic tool for HIV. |
| Publikationsart: | Article |
| Sprache: | English |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.4c03374 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/39314132 |
| Rights: | STM Policy #29 |
| Dokumentencode: | edsair.doi.dedup.....1297bc00384f2acdae5465234a10e7d6 |
| Datenbank: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | The noncanonical conformations within the genomes of viral pathogens is of significant diagnostic value, due to their unique secondary structures and interactions with specific fluorogenic molecules. In particular, adaptation of the G-quadruplex (GQ) conformation by the specific gene sequence leads to distinct topological features, resulting in unique binding sites that are crucial for the selective recognition of human immunodeficiency virus (HIV) by small molecules. Leveraging the selective fluorescence response of a benzobisthiazole-based fluorogenic probe to the LTR-III GQ target, we developed a GQ-based diagnostic platform for HIV detection. The successful fluorescence recognition of an amplified 176-nucleotide genomic segment harboring the LTR-III GQ, facilitated by pH-controlled GQ-targeted reliable conformational polymorphism (GQ-RCP), validates this method as an effective GQ-topology-targeted diagnostic tool for HIV. |
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| ISSN: | 15206882 00032700 |
| DOI: | 10.1021/acs.analchem.4c03374 |