Zobrazit v EDS

Clinical Characteristics, Laboratory Parameters, and Molecular Epidemiology of Neuroinvasive Flavivirus Infections in a Hotspot Region of Eastern Croatia

Uloženo v:
Podrobná bibliografie
Název: Clinical Characteristics, Laboratory Parameters, and Molecular Epidemiology of Neuroinvasive Flavivirus Infections in a Hotspot Region of Eastern Croatia
Autoři: Dario Sabadi, Kristian Bodulić, Vladimir Savić, Nika Vlahović Vlašić, Maja Bogdanić, Ljiljana Perić, Irena Tabain, Dubravka Lišnjić, Mario Duvnjak, Snježana Židovec-Lepej, Barbara Grubišić, Ilija Rubil, Ljubo Barbić, Luka Švitek, Vladimir Stevanović, Petra Smajić, Bernarda Berišić, Mihaela Zlosa, Ivana Rončević, Tatjana Vilibić-Čavlek
Zdroj: Pathogens
Pathogens, Vol 14, Iss 1, p 69 (2025)
Informace o vydavateli: MDPI AG, 2025.
Rok vydání: 2025
Témata: Croatia / epidemiology, West Nile virus / isolation & purification, Male, Adult, West Nile Fever / epidemiology, Flavivirus Infections / virology, Flavivirus Infections / epidemiology, Croatia, Immunoglobulin G / blood, neuroinvasive disease, Antibodies, Viral, Article, Encephalitis Viruses, Tick-Borne / isolation & purification, Flavivirus Infections, Encephalitis Viruses, Tick-Borne, Antibodies, Viral / blood, Young Adult, West Nile Fever / virology, Immunoglobulin M / blood, Humans, clinical characteristics, Aged, Aged, 80 and over, Molecular Epidemiology, Encephalitis, Tick-Borne / epidemiology, tick-borne encephalitis, Flavivirus, Encephalitis Viruses, Tick-Borne / genetics, Encephalitis, Tick-Borne / virology, Middle Aged, Immunoglobulin M, Immunoglobulin G, Encephalitis Viruses, Tick-Borne / immunology, laboratory parameters, Medicine, RNA, Viral, Antibodies, Viral / cerebrospinal fluid, RNA, Viral / genetics, Female, Flavivirus / genetics, West Nile virus / genetics, West Nile virus, Encephalitis, Tick-Borne, West Nile Fever
Popis: Neuroinvasive flaviviruses such as tick-borne encephalitis virus (TBEV) and West Nile virus (WNV) are widely distributed in continental Croatian regions. We analyzed clinical characteristics, laboratory parameters, and molecular epidemiology of neuroinvasive flavivirus infections in eastern Croatia. A total of 43 patients with confirmed flavivirus infection hospitalized from 2017 to 2023 were included in the study. Reverse-transcription polymerase chain reaction (RT-qPCR) was used to detect flavivirus RNA in clinical samples (cerebrospinal fluid; CSF, urine). ELISA was used for IgM and IgG antibody detection in serum and CSF with confirmation of cross-reactive samples by virus neutralization test. WNV was detected more frequently (74.4%) than TBEV (25.6%). A statistically significant age difference was found between WNV patients (median 65 years) and TBEV patients (median 36 years). Comorbidities were more frequently detected in WNV patients (hypertension 56.3 vs. 18.2%; diabetes 31.3 vs. 0%). Meningitis was the most common clinical presentation in both TBE and WNV neuroinvasive disease (WNND; 63.6 and 59.4%, respectively). In addition, some rare clinical presentations of WNND were also detected (cerebellitis, polyradiculoneuritis). No significant differences in the frequency of clinical symptoms were observed between WNV and TBEV-infected patients (fever 93.7 vs. 100%; malaise 78.1 vs. 100%; headache 75.0 vs. 100%; nausea 50.0 vs. 63.6%; vomiting 34.4 vs. 54.6%). Comparative analysis of total and differential leukocyte blood count showed similar results. However, CSF pleocytosis was higher in TBE patients, with a significant difference in the neutrophil and lymphocyte count (WNND median 48.5% and 51.5%; TBE median 10.0 and 90.0%, respectively). The length of hospital stay was 12 days for WNND and 9 days for TBE. Phylogenetic analysis of detected WNV strains revealed the presence of WNV lineage 2 in eastern Croatia.
Druh dokumentu: Article
Other literature type
Popis souboru: application/pdf
Jazyk: English
ISSN: 2076-0817
DOI: 10.3390/pathogens14010069
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39861030
https://doaj.org/article/ed6567b85cc7404d8c9f30a39249af2c
https://www.mdpi.com/2076-0817/14/1/69
https://doi.org/10.3390/pathogens14010069
https://urn.nsk.hr/urn:nbn:hr:105:423799
https://doi.org/10.3390/pathogens14010069
Rights: CC BY
Přístupové číslo: edsair.doi.dedup.....0c353a07124ed6cca2e97994cc500d24
Databáze: OpenAIRE
Popis
Abstrakt:Neuroinvasive flaviviruses such as tick-borne encephalitis virus (TBEV) and West Nile virus (WNV) are widely distributed in continental Croatian regions. We analyzed clinical characteristics, laboratory parameters, and molecular epidemiology of neuroinvasive flavivirus infections in eastern Croatia. A total of 43 patients with confirmed flavivirus infection hospitalized from 2017 to 2023 were included in the study. Reverse-transcription polymerase chain reaction (RT-qPCR) was used to detect flavivirus RNA in clinical samples (cerebrospinal fluid; CSF, urine). ELISA was used for IgM and IgG antibody detection in serum and CSF with confirmation of cross-reactive samples by virus neutralization test. WNV was detected more frequently (74.4%) than TBEV (25.6%). A statistically significant age difference was found between WNV patients (median 65 years) and TBEV patients (median 36 years). Comorbidities were more frequently detected in WNV patients (hypertension 56.3 vs. 18.2%; diabetes 31.3 vs. 0%). Meningitis was the most common clinical presentation in both TBE and WNV neuroinvasive disease (WNND; 63.6 and 59.4%, respectively). In addition, some rare clinical presentations of WNND were also detected (cerebellitis, polyradiculoneuritis). No significant differences in the frequency of clinical symptoms were observed between WNV and TBEV-infected patients (fever 93.7 vs. 100%; malaise 78.1 vs. 100%; headache 75.0 vs. 100%; nausea 50.0 vs. 63.6%; vomiting 34.4 vs. 54.6%). Comparative analysis of total and differential leukocyte blood count showed similar results. However, CSF pleocytosis was higher in TBE patients, with a significant difference in the neutrophil and lymphocyte count (WNND median 48.5% and 51.5%; TBE median 10.0 and 90.0%, respectively). The length of hospital stay was 12 days for WNND and 9 days for TBE. Phylogenetic analysis of detected WNV strains revealed the presence of WNV lineage 2 in eastern Croatia.
ISSN:20760817
DOI:10.3390/pathogens14010069