Illuminating Extracellular Vesicles Biology with Super-Resolution Microscopy: Insights into Morphology and Composition
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| Název: | Illuminating Extracellular Vesicles Biology with Super-Resolution Microscopy: Insights into Morphology and Composition |
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| Autoři: | Lucile Alexandre, Daniele D’Arrigo, Nicolas Kuszla, André Cronemberger Andrade, Amanda K. A. Silva, Cataldo Schietroma, Florence Gazeau, Quentin Lubart, Stéphanie Mangenot |
| Zdroj: | ACS Nano. 19:24148-24173 |
| Publication Status: | Preprint |
| Informace o vydavateli: | American Chemical Society (ACS), 2025. |
| Rok vydání: | 2025 |
| Témata: | Biological Physics (physics.bio-ph), FOS: Physical sciences, Physics - Biological Physics |
| Popis: | Super-Resolution Microscopy (SRM) is emerging as a powerful and innovative tool for imaging, characterizing, and understanding the structure of Extracellular Vesicles (EVs). By addressing the need for single-particle analysis with the high resolution required to study the composition and organization of these nanoparticles, SRM provides unique insights into EV biology. However, its application is accompanied by significant challenges, ranging from experimental setup to data analysis. This review outlines the fundamentals of SRM and its position within the broader field of EV research. We then explore its applications in evaluating (i) the morphological structure of EVs, (ii) their molecular composition, and (iii) their roles in biological systems. By offering practical guidance and an overview of critical parameters for standardization, this review aims at providing researchers with the tools and insights necessary to effectively apply SRM to EV investigation. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.5c00380 |
| DOI: | 10.48550/arxiv.2501.03713 |
| Přístupová URL adresa: | http://arxiv.org/abs/2501.03713 |
| Rights: | STM Policy #29 CC BY NC ND |
| Přístupové číslo: | edsair.doi.dedup.....097069b86e97b06cc2ee6d1a106a3eaa |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Super-Resolution Microscopy (SRM) is emerging as a powerful and innovative tool for imaging, characterizing, and understanding the structure of Extracellular Vesicles (EVs). By addressing the need for single-particle analysis with the high resolution required to study the composition and organization of these nanoparticles, SRM provides unique insights into EV biology. However, its application is accompanied by significant challenges, ranging from experimental setup to data analysis. This review outlines the fundamentals of SRM and its position within the broader field of EV research. We then explore its applications in evaluating (i) the morphological structure of EVs, (ii) their molecular composition, and (iii) their roles in biological systems. By offering practical guidance and an overview of critical parameters for standardization, this review aims at providing researchers with the tools and insights necessary to effectively apply SRM to EV investigation. |
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| ISSN: | 1936086X 19360851 |
| DOI: | 10.1021/acsnano.5c00380 |