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Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe

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Název: Photopharmacological modulation of hippocampal local field potential by caged‐glutamate with MicroLED probe
Autoři: Shogo Okada, Noriaki Ohkawa, Kazuki Moriya, Yoshito Saitoh, Mikiko Ishikawa, Kakeru Oya, Atsushi Nishikawa, Hiroto Sekiguchi
Zdroj: Neuropsychopharmacol Rep
Neuropsychopharmacology Reports, Vol 44, Iss 3, Pp 658-662 (2024)
Informace o vydavateli: Wiley, 2024.
Rok vydání: 2024
Témata: Male, 0301 basic medicine, 0303 health sciences, Light, hippocampus, Glutamic Acid, Neurosciences. Biological psychiatry. Neuropsychiatry, RM1-950, Hippocampus, local field potential (LFP), Mice, Inbred C57BL, Mice, 03 medical and health sciences, microscale light‐emitting diode (MicroLED), Dentate Gyrus, Animals, photopharmacology, Original Article, Therapeutics. Pharmacology, caged‐glutamate, RC321-571
Popis: AimPhotopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation‐dependent manner. Here, we investigated whether a microscale light‐emitting diode (MicroLED) probe is applicable for the photoconversion of caged‐glutamate (caged‐Glu) in vivo.MethodsA needle‐shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged‐Glu was infused into the DG and illuminated with light from a MicroLED probe.ResultsIn the caged‐Glu‐injected DG, the LFP changed in the 10–20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition.ConclusionThe MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged‐Glu in vivo.
Druh dokumentu: Article
Other literature type
Jazyk: English
ISSN: 2574-173X
DOI: 10.1002/npr2.12472
Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39126158
https://doaj.org/article/105c94baef6b4708bda2a9be49a59714
Rights: CC BY NC ND
URL: http://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Přístupové číslo: edsair.doi.dedup.....0956378d77e5c04bf23ef452538b37a1
Databáze: OpenAIRE
Popis
Abstrakt:AimPhotopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation‐dependent manner. Here, we investigated whether a microscale light‐emitting diode (MicroLED) probe is applicable for the photoconversion of caged‐glutamate (caged‐Glu) in vivo.MethodsA needle‐shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged‐Glu was infused into the DG and illuminated with light from a MicroLED probe.ResultsIn the caged‐Glu‐injected DG, the LFP changed in the 10–20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition.ConclusionThe MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged‐Glu in vivo.
ISSN:2574173X
DOI:10.1002/npr2.12472