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Contribution of Heme Oxygenase 2 to Blood Pressure Regulation in Response to Swimming Exercise and Detraining in Spontaneously Hypertensive Rats

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Titel: Contribution of Heme Oxygenase 2 to Blood Pressure Regulation in Response to Swimming Exercise and Detraining in Spontaneously Hypertensive Rats
Autoren: Vural Kucukatay, Melek Bor-Kucukatay, Hande Senol, Gurkan Turhan, Gulcin Abban-Mete, Emine Kilic-Toprak, Vildan Caner, Ozgen Kilic-Erkek, Oktay Kuru, Ikbal Cansu Baris
Weitere Verfasser: MÜ
Quelle: Med Sci Monit
Verlagsinformationen: International Scientific Information, Inc., 2018.
Publikationsjahr: 2018
Schlagwörter: Male, 0301 basic medicine, systolic blood pressure, Wistar, complementary DNA, peroxidase, Blood Pressure, Wistar rat, adventitia, Random Allocation, 0302 clinical medicine, endurance training, Rats, Inbred SHR, histological score, carboxyhemoglobin, Animals, Aorta/enzymology/physiology, Blood Pressure/*physiology, Carbon Monoxide/metabolism, Carboxyhemoglobin/metabolism, Heme Oxygenase (Decyclizing)/metabolism/physiology, Hypertension/*enzymology/physiopathology, Physical Conditioning, Animal/physiology, Rats, Rats, Wistar, Swimming/*physiology, rat, animal, swimming, pathophysiology, Aorta, Carbon Monoxide, training, exercise, messenger RNA, blood pressure regulation, blood pressure, Morris water maze test, Heme oxygenase (Decyclizing), heme oxygenase, Physical Conditioning, beta actin, enzyme activity, aerobic exercise, blood gas analysis, protein expression level, Carboxyhemoglobin, real time polymerase chain reaction, immunohistochemistry, Hypertension, heme oxygenase 2, spontaneously hypertensive rat, Inbred SHR, hypertension, enzymology, animal experiment, randomization, carbon monoxide, Article, animal tissue, 03 medical and health sciences, male, Physical Conditioning, Animal, controlled study, Swimming, nonhuman, Animal Study, essential hypertension, sitting, scoring system, RNA extraction, aorta, physiology, Heme Oxygenase (Decyclizing), gene expression, mRNA expression level, immunoperoxidase staining, metabolism
Beschreibung: BACKGROUND We aimed to determine the effects of exercise followed by detraining on systolic blood pressure (SBP), heme oxygenase 2 (HO-2) expression, and carboxyhemoglobin (COHb) concentration in spontaneously hypertensive rats (SHR) to explain the role of carbon monoxide (CO) in this process. MATERIAL AND METHODS Animals were randomized into exercised and detrained groups. Corresponding sedentary rats were grouped as Time 1-2. Swimming of 60 min/5 days/week for 10 weeks was applied. Detraining rats discontinued training for an additional 5 weeks. Gene and protein expressions were determined by real-time PCR and immunohistochemistry. RESULTS Aorta HO-2 histological scores (HSCORE) of hypertensive rats were lower, while SBP was higher. Swimming caused enhancement of HO-2 immunostaining in aorta endothelium and adventitia of SHR. Exercise induced elevation of blood COHb index in SHR. Synchronous BP lowering effect of exercise was observed. HO-2 mRNA expression, HSCORE, and blood COHb index were unaltered during detraining, while SBP was still low in SHR. CONCLUSIONS CO synthesized by HO-2 at least partly plays a role in SBP regulation in the SHR- and BP-lowering effect of exercise. Regular exercise with short-term pauses may be advised to both hypertensives and individuals who are at risk.
Publikationsart: Article
Other literature type
Dateibeschreibung: application/pdf
Sprache: English
ISSN: 1643-3750
DOI: 10.12659/msm.908992
Zugangs-URL: https://europepmc.org/articles/pmc6116639?pdf=render
https://pubmed.ncbi.nlm.nih.gov/30132448
http://europepmc.org/articles/PMC6116639
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6116639
https://pubmed.ncbi.nlm.nih.gov/30132448/
https://hdl.handle.net/11499/10675
https://doi.org/10.12659/msm.908992
http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/10675
Rights: CC BY NC ND
Dokumentencode: edsair.doi.dedup.....0613a521a5c3445f66134024f18db878
Datenbank: OpenAIRE
Beschreibung
Abstract:BACKGROUND We aimed to determine the effects of exercise followed by detraining on systolic blood pressure (SBP), heme oxygenase 2 (HO-2) expression, and carboxyhemoglobin (COHb) concentration in spontaneously hypertensive rats (SHR) to explain the role of carbon monoxide (CO) in this process. MATERIAL AND METHODS Animals were randomized into exercised and detrained groups. Corresponding sedentary rats were grouped as Time 1-2. Swimming of 60 min/5 days/week for 10 weeks was applied. Detraining rats discontinued training for an additional 5 weeks. Gene and protein expressions were determined by real-time PCR and immunohistochemistry. RESULTS Aorta HO-2 histological scores (HSCORE) of hypertensive rats were lower, while SBP was higher. Swimming caused enhancement of HO-2 immunostaining in aorta endothelium and adventitia of SHR. Exercise induced elevation of blood COHb index in SHR. Synchronous BP lowering effect of exercise was observed. HO-2 mRNA expression, HSCORE, and blood COHb index were unaltered during detraining, while SBP was still low in SHR. CONCLUSIONS CO synthesized by HO-2 at least partly plays a role in SBP regulation in the SHR- and BP-lowering effect of exercise. Regular exercise with short-term pauses may be advised to both hypertensives and individuals who are at risk.
ISSN:16433750
DOI:10.12659/msm.908992