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Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights

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Title: Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
Authors: Duzel, Antonija, Vlainić, Josipa, Antunović, Marko, Malekinušić, Dominik, Vrdoljak, Borna, Samara, Mariam, Gojković, Slaven, Krežić, Ivan, Vidović, Tinka, Bilić, Zdenko, Knežević, Mario, Sever, Marko, Lojo, Nermin, Kokot, Antonio, Kolovrat, Marijan, Drmić, Domagoj, Vukojević, Jakša, Kralj, Tamara, Kasnik, Katarina, Siroglavić, Marko, Seiwerth, Sven, Sikirić, Predrag
Source: World J Gastroenterol
Publisher Information: Baishideng Publishing Group Inc., 2017.
Publication Year: 2017
Subject Terms: Male, 0301 basic medicine, L-arginine, blood flow rescue, BPC 157, Malondialdehyde, Colon/blood supply, NG-Nitroarginine Methyl Ester/therapeutic use, Colon/drug effects, oxidative stress, Nitric Oxide/analysis, Colitis/pathology, 0303 health sciences, Collaterals, Blood flow rescue, Basic Study, Proteins/pharmacology, Colitis, Colon/metabolism, Arginine/therapeutic use, 3. Good health, Collateral Circulation/drug effects, NG-Nitroarginine Methyl Ester, Reperfusion Injury, Reperfusion Injury/pathology, Anti-Ulcer Agents/therapeutic use, Colon, Oxidative Stress/drug effects, Collateral Circulation, ischemic colitis, Arginine, Nitric Oxide, NO, 03 medical and health sciences, L-NAME, Animals, Humans, Colitis/etiology, Reperfusion Injury/etiology, Malondialdehyde/blood, Rats, Wistar, Peptide Fragments/therapeutic use, Ischemic colitis, Oxidative stress, Rats, Peptide Fragments/pharmacology, collaterals, Proteins, Colitis/drug therapy, Anti-Ulcer Agents, Peptide Fragments, rats, Disease Models, Animal, Oxidative Stress, Proteins/therapeutic use, Reperfusion Injury/drug therapy, Anti-Ulcer Agents/pharmacology, Colitis/blood, Reperfusion Injury/blood
Description: To provide new insights in treatment of colitis and ischemia and reperfusion in rats using stable gastric pentadecapeptide BPC 157.Medication [BPC 157, L-NAME, L-arginine (alone/combined), saline] was bath at the blood deprived colon segment. During reperfusion, medication was BPC 157 or saline. We recorded (USB microscope camera) vessel presentation through next 15 min of ischemic colitis (IC-rats) or reperfusion (removed ligations) (IC + RL-rats); oxidative stress as MDA (increased (IC- and IC + RL-rats)) and NO levels (decreased (IC-rats); increased (IC + RL-rats)) in colon tissue. IC + OB-rats [IC-rats had additional colon obstruction (OB)] for 3 d (IC + OB-rats), then received BPC 157 bath.Commonly, in colon segment (25 mm, 2 ligations on left colic artery and vein, 3 arcade vessels within ligated segment), in IC-, IC + RL-, IC + OB-rats, BPC 157 (10 μg/kg) bath (1 mL/rat) increased vessel presentation, inside/outside arcade interconnections quickly reappeared, mucosal folds were preserved and the pale areas were small and markedly reduced. BPC 157 counteracted worsening effects induced by L-NAME (5 mg) and L-arginine (100 mg). MDA- and NO-levels were normal in BPC 157 treated IC-rats and IC + RL-rats. In addition, on day 10, BPC 157-treated IC + OB-rats presented almost completely spared mucosa with very small pale areas and no gross mucosal defects; the treated colon segment was of normal diameter, and only small adhesions were present.BPC 157 is a fundamental treatment that quickly restores blood supply to the ischemically injured area and rapidly activates collaterals. This effect involves the NO system.
Document Type: Article
Other literature type
File Description: application/pdf
ISSN: 1007-9327
DOI: 10.3748/wjg.v23.i48.8465
Access URL: https://pubmed.ncbi.nlm.nih.gov/29358856
https://www.ncbi.nlm.nih.gov/pubmed/29358856
https://doi.org/10.3748/wjg.v23.i48.8465
https://europepmc.org/article/MED/29358856
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752708
https://www.ncbi.nlm.nih.gov/pubmed/29358856
https://www.bib.irb.hr/981047
https://www.wjgnet.com/1007-9327/full/v23/i48/8465.htm
http://doi.org/10.3748/wjg.v23.i48.8465
Rights: CC BY NC
URL: http://rightsstatements.org/vocab/InC/1.0/
Accession Number: edsair.doi.dedup.....04f3f880274dcac926dcf3d3e6f59ede
Database: OpenAIRE
Description
Abstract:To provide new insights in treatment of colitis and ischemia and reperfusion in rats using stable gastric pentadecapeptide BPC 157.Medication [BPC 157, L-NAME, L-arginine (alone/combined), saline] was bath at the blood deprived colon segment. During reperfusion, medication was BPC 157 or saline. We recorded (USB microscope camera) vessel presentation through next 15 min of ischemic colitis (IC-rats) or reperfusion (removed ligations) (IC + RL-rats); oxidative stress as MDA (increased (IC- and IC + RL-rats)) and NO levels (decreased (IC-rats); increased (IC + RL-rats)) in colon tissue. IC + OB-rats [IC-rats had additional colon obstruction (OB)] for 3 d (IC + OB-rats), then received BPC 157 bath.Commonly, in colon segment (25 mm, 2 ligations on left colic artery and vein, 3 arcade vessels within ligated segment), in IC-, IC + RL-, IC + OB-rats, BPC 157 (10 μg/kg) bath (1 mL/rat) increased vessel presentation, inside/outside arcade interconnections quickly reappeared, mucosal folds were preserved and the pale areas were small and markedly reduced. BPC 157 counteracted worsening effects induced by L-NAME (5 mg) and L-arginine (100 mg). MDA- and NO-levels were normal in BPC 157 treated IC-rats and IC + RL-rats. In addition, on day 10, BPC 157-treated IC + OB-rats presented almost completely spared mucosa with very small pale areas and no gross mucosal defects; the treated colon segment was of normal diameter, and only small adhesions were present.BPC 157 is a fundamental treatment that quickly restores blood supply to the ischemically injured area and rapidly activates collaterals. This effect involves the NO system.
ISSN:10079327
DOI:10.3748/wjg.v23.i48.8465