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A guide to selecting high-performing antibodies for ADNP (UniProt ID: Q9H2P0) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 2 approved, 1 approved with reservations]

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Název: A guide to selecting high-performing antibodies for ADNP (UniProt ID: Q9H2P0) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 2 approved, 1 approved with reservations]
Autoři: Carl Laflamme, Vera Ruíz Moleón, Sara González Bolívar, Riham Ayoubi, Charles Alende, Maryam Fotouhi
Zdroj: F1000Research, Vol 13 (2024)
Informace o vydavateli: F1000 Research Ltd, 2024.
Rok vydání: 2024
Témata: eng, antibody characterization, Science, Medicine, antibody validation, Activity-Dependent Neuroprotective Protein, Activity-Dependent Neuroprotector homeobox Protein, ADNP, Q9H2P0
Popis: ADNP is a multifunctional protein involved in chromatin remodeling, transcription, and microtubule interaction, playing a critical role in brain development, with mutations linked to ADNP-Related Disorder. Here we have characterized seven ADNP commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
Druh dokumentu: Article
Jazyk: English
ISSN: 2046-1402
DOI: 10.12688/f1000research.160121.1
Přístupová URL adresa: https://doaj.org/article/591642ca03754b0b80498730b1c9ddf8
Přístupové číslo: edsair.doajarticles..a46d5bb256e98744ac57cd7a3190f7d5
Databáze: OpenAIRE
Popis
Abstrakt:ADNP is a multifunctional protein involved in chromatin remodeling, transcription, and microtubule interaction, playing a critical role in brain development, with mutations linked to ADNP-Related Disorder. Here we have characterized seven ADNP commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
ISSN:20461402
DOI:10.12688/f1000research.160121.1